Latest Issue
Vol. 6 ， Issue 4 ， 2000
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- Abstract：Objective: To investigate the molecular mechanism of Liver Yang Ascending Syndrome (LYAS) in hypertension.Methods: The plasma norepinephrine (NE), epinephrine (E) contents in patients with LYAS or Liver and Kidney Yin Deficiency Syndrome ( LKYDS) of hypertension and normal controls were determined by high performance liquid chromatography-electrochemical detector (HPLC-ECD). The polymorphism of tyrosine hydroxylase (TH) gene in the three groups were analysed by Southern Blot assay. The polymorphism of TH and monoamine oxidation A(MAO-A), monoamine oxidation B(MAO-B) gene microsatellite in these groups were analysed by polymerase chain reaction (PCR)-SSCP. At the same time, the LYAS model was established by administering Aconitum preparation (AP) to spontaneous hypertension rats (SHR). The adrenal gland medulla of the experimental animal was examined for their TH expression at the protein level after ABC immuno-histochemical staining with the TH monoclonal antibody (McAb). The adrenal TH gene mRNA expression was demonstrated by in situ hybridization with the synthetized TH oligonucleic acid probe. The results of immuno-histochemistry and in situ hybridization were analyzed with the image analysis system ( IAS).Results: The plasma NE and E contents in patients with LYAS were more obviously increased than those in LKYDS and normal controls. The TH gene amplified and the type A1 TH microsatellite D11S4046 was markedly higher in the LYAS group than those in the other groups. The TH mRNA protein expression in the adrenal tissue of the AP induced LYAS model in SHR elevated.Conclusion: The LYAS of hypertension has the characters as amplified TH gene and increased TH mRNA and protein expression, which suggested that the overex-pression of TH is probably the mechanism of LYAS in hypertension.Keywords：hypertension;Liver Yang Ascending Syndrome;Liver and Kidney Yin Deficiency Syndrome;Tyrosine Hydroxylase;molecular mechanism
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Published：2021-08-27 - Abstract：Objective: To study the effect of rhubarb on gastrointestinal (GI) blood flow perfussion.Methods: State of GI blood flow perfussion in critically illed patients was estimated by intramural pH (pHi) of GI to observe the clinical effect of rhubarb on it, and experimental study was performed in hemorrhagic shocked rats.Results: Clinical study showed pHi level in septic patients was much lower than that in the control, whereas rhubarb could obviously elevate it (P<0.001). The clinical effective rate of rhubarb in treating stress ulcer caused hemorrhage was 73 %. Animal study showed that though the shocked rats were resuscitated completely, their GI blood flow perfussion was much lower than that in the control. Rhubarb could significantly improve the blood perfusion of mucous membrane of GI and mesenterium (P<0.01) in shocked or normal rats.Conclusion: Rhubarb could improve the GI blood perfussion in critical illness and shocked rats.Keywords：Tyrosine Hydroxylase;Septic Patient;Liquid Chromatographic Determination;Tyrosine Hydroxylase Gene;Illed Patient
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Published：2021-08-27 - Abstract：Objective: To explore the effect of composite Salviae droping pill(CSDP) on endothelin (ET-1) gene expression in circulating endothelial cells.Mothods: Seventy cases of stable angina pectoris were randomly divided into two groups, the CSDP group and the isosorbide dinitrate (ID) group. They were treated with CS-DP and ID respectively, their ET-1 gene expression in endothelial cells of peripheral circulation was measured before and after treatment by reverse transcriptase-polymerase chain reaction(RT-PCR) and compared between the two groups as well as with that of healthy subjects.Results: Electrophoresis banding of 546 bp cDNA procured from 59 cases of 70 patients was positive, while no positive banding was obtained from the healthy subjects. Six cases from the 29 patients treated with CSDP had their banding turned to negative, while in the ID group, no one turned to negative after treatment. And ET-1 PCR product (absorbed optic density) in the CSDP group was markedly lower than that in the ID group, P>0.05.Conclusion: DSP could directly inhibit ET-1 gene expression in endothelial cells of peripheral circulation.Keywords：endothelin;gene expression;composite Salviae dropping pill;circulating endothelial cell
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Published：2021-08-27 - Abstract：Objective: To explore the effect of Xuefu Zhuyu Decoction (XZD) on molecular expression of platelets glycoprotein II b/III a complex (GP II b/III a) and thrombomodulin (TM) of human umbilical vein endothelial cells.Methods: Platelets and human umbilical vein endothelial cells were incubated with XZD of different concentration. GP II b/III a and TM were evaluated by radioimmunoassay.Results: XZD in 40 mg/ml and 80 mg/ml could obviously inhibit the adenosine diphosphate induced GII b/III a complex expression, the molecular number being 52900 ± 8445 and 52095 ± 6345 respectively, and there was significant difference as comparing with that in the control group ( P < 0.05, P<0.01). But XZD didn’t show any influence on the molecular expression of TM in human umbilical vein endothelial cells.Conclusion: XZD could inhibit the adenosine diphosphate induced activation of platete through blocking the exposure of GII b/III a a complex.Keywords：platelet glycoprotein II b/III a complex;thrombomodulin (TM)kw]human umbilical vein endothelial cell
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Published：2021-08-27 - Abstract：Objective: To study the mechanism of acupuncture in treating simple obesity.Methods: Central nerve push-pull perfusion and biochemical technique were used to observe the effect of acupuncture on the obese parameters, changes of monoamine transmitters and activity of ATPase in the lateral hypothalamic area (LHA) of obese rats.Results: Noradrenaline (NA) level in LHA of obese rats was higher but serotonin (5-HT) level and ATPase activity were lower than those in normal rats. After acupuncture treatment, in the same time of reducing body weight, NA level in LHA of rats was reduced, and 5-HT level and ATPase activity in it were increased. (P<0.05 and P< 0.01).Conclusion : The effective regulation on LHA of obese rats is possibly one of the key factors in anti-obesity effect of acupuncture.Keywords：Acupuncture Treatment;Lateral Hypothalamic Area;Blood Stasis Syndrome;simple obesity;Free Acid Form
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Published：2021-08-27 - Abstract：Objective: To observe the clinical effect of integrated traditional Chinese and western medicine (TCM-WM) in treating senile idiopathic nephrotic syndrome (SINS).Methods: Eighty-two patients of SINS were randomly divided into two groups. The 41 patients in the TCM-WM group were treated with prednisone, Cytoxan (CTX) and Chinese medicinal herbs according to Syndrome-type, and the 41 patients in the control group were treated with western medicine alone. The clinical remission rate and time, recurrence rate in patients as well as adverse reaction rate occurred were observed.Results: The complete remission rate and total effective rate of the TCM-WM group were 58.5% and 85.4 %, which were significantly higher than those of the control group (39.0% and 63.4 %,P < 0.01 ). The adverse reaction rate in the TCM-WM group was 36.6 %, which was significantly lower than that of the control group (80.5%, P<0.01). A follow-up of 35.1 ± 9. 7 months showed that there was no significant difference in the recurrence rate between the two groups (P > 0.05 ), but the average remission period (30.6 ± 3.8 months) of the TCM-WM group was significantly longer than that of the control group (12.7±3.7 months, P<0.01).Conclusion: TCM-WM treatment is obviously superior to that with western medicine alone in increasing clinical remission rate, relieving adverse reaction rate and extending remission period.Keywords：integrated traditional Chinese and Western medicine;senility;idiopathic nephrotic syndrome
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Published：2021-08-27 - Abstract：Objective: To investigate the mechanism of protective effect of Astragalus (As) plus ligustrazine (Lt) against myocardial ischemia reperfusion injury (MIRI) during open heart surgery of cardiopulmonary bypass, and the treating principle of MIRI in TCM.Methods: Twenty-four patients were randomly divided into three treated groups and treated with As injection, Lt injection and both (As + Lt) respectively, 6 in each group, and the remainder 6 were taken as control group. Blood samples of them were collected before anesthesia (T1), 10 minutes after occlusion of aorta (T2), 10 minutes(T3) and 30 minutes(T4) after the release, and end of operation (about 180 minutes after release, T5) respectively to test the levels of aspartate aminotransferase ( AST ), lactate dehydrogenase (LDH), creatine kinase (CK), MB isoenzyme of CK (CK-MB), malondialdehyde (MDA) and the activity of Superoxide dismutase ( SOD ), nitric oxide ( NO ), nitric oxide synthetase (NOS).Results: The levels of AST, LDH, CK were reduced in the treated groups, showing significant difference (P< 0.05 or P < 0. 01 ) as compared with those in the control groups. Among them the effect in the AS + Lt group was the best. And NO activity in As group at T5 and As + Lt group at T4, T5 was higher than that in the control group.Conclusion : The mechanism of MIRI is Qi deficiency and blood stasis in TCM, its treating principles should be promoting Qi and removing blood stasis. According to the authors’ study, combined As + Lt therapy is more effective in protecting against MIRI than that using the 2 medicines separately.Keywords：Nitric Oxide;Creatine Kinase;Open Heart Surgery;Blood Stasis;nitric oxide synthetase
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Published：2021-08-27 - Abstract：Objective: To investigate the effects of Astragalus membranaceus on patients with incipient diabetic nephropathy( IDN).Methods: Forty patients with IDN were randomly divided into two groups. The twenty cases in the treated group (Group A) were treated with Astragalus membranaceus injection 20 ml/d (equivalent to Astragalus membranaceus 40 g) in addition to conventional therapy, whereas the 20 cases in the control group(Group B) were treated only with conventional therapy. The levels of urinary endothelin (UET), and albumin were measured before and after treatment. Blood glucose and glycosylated hemoglobulin levels were also measured.Results: After four weeks therapy, the levels of UET and urinary albumin excretion rate. (UAER) in Group A were 39.8 ± 12.7 pg/12h and 37.3 ± 30.4 μg/min respectively as compared with Group B (51.2±12.8pg/12h, 79.6 ± 35.8μg/min respectively, P<0.05, P<0.01 respectively). Compared with the pretreatment level, the UET and UAER values declined significantly in Group A(P< 0.01), but not in Group B(P>0. 05). One month after stopping use of Astragalus membranaceus, the levels of UET and UAER in Group A were still lower than those in Group B, and the difference in the two groups was statistically significant.Conclusion: Astragalus membranaceus might reduce UET and UAER in patients with IDN.Keywords：Astragalus membranaceus;incipient diabetic nephropathy;urinary endothelin;microalbuminuria
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Published：2021-08-27 -
Clinical study of ectopic pregnancy treated with integrated traditional chinese and western medicine
Abstract：Objective: To explore the therapeutic effect and mechanism of integrated traditional Chinese and western medicine (TCM-WM) in treating ectopic pregnancy (EP).Methods: Seventy-eight patients suffering from unruptured EP were divided into two groups at random in proportion of roughly 2:1, Group I (n= 50) was treated with methotrexate (MTX) plus Ectopic Pregnancy No. 2 (EP2), a Chinese medicinal decoction, Group II (n=28) was treated with MTX alone for control.Results:In Group I, the nonsurgical cure rate, involving side tubal patency rate and intrauterine pregnancy rate, were 98. 0%, 88. 0% and 80. 0% respectively, the three parameters were all higher than those in Group II (75. 0%, 46. 4% and 42. 9% respectively). Moreover, the EP recurrence rate in Group I was lower than that in Group II (4.0% vs l4.2%), the serum β-hCG normalizing time and total disappearance time of EP mass in Group I (25.0 ± 7. 8 days and 1.2 ± 0.7 months) were shorter than those in Group II (31.9 ± 6.7 days and 5.6 + 3.7 months), also showing significant difference (P<0. 05 and P<0. 01 respectively). After being treated by EP2 decoction for 4–8 weeks, the hemorrheological parameters in Group I were improved more markedly than those in Group II.Conclusion: The TCM-WM treatment (MTX plus EP2) could effectively increase nonsurgical cure rate, tubal patency rate and intrauterine pregnancy rate, decrease EP recurrence rate, and reduce the serum β-hCG normalizing time and the total disappearance time of EP mass in treating EP.Keywords：ectopic pregnancy;Ectopic Pregnancy No. 2;methotrexate- 747
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Published：2021-08-27 - Abstract：Objective: To compare the therapeutic effect of Shenmai injection (SM) and aminophylline (AP) on diaphragm fatigue in chronic obstructive pulmonary disease ( COPD ).Methods: Sixty-four COPD patients with respiratory failure and diaphragm fatigue were randomly divided into 2 groups. The SM group (n=33) was treated with oxygen inhalation, anti-infection agents, and SM injection (SM 40ml + 10% glucose 100ml). The AP group (n=31) was treated by the same comprehensive treatment but with AP 0. 25g instead of SM. The initial time of diaphragm fatigue disappearance, 24 hrs maintaining effect and the arterial blood gas analysis were observed.Results: The effect in 30 min and maintaining effect in 24 hrs in SM group were better than those in AP group. In both groups, PaO2 increased and PaCO2 decreased.Conclusion: The therapeutic effect of SM on COPD patients with respiratory failure and diaphragm fatigue was better than those of AP.Keywords：Chronic Obstructive Pulmonary Disease;Chronic Obstructive Pulmonary Disease Patient;Extrauterine Pregnancy;Diaphragm Fatigue;Interstitial Pregnancy
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Published：2021-08-27 - Abstract：Objective: To evaluate the therapeutic effect of Juanxiao Tablet (JXT) in treating asthma.Methods: Clinical observation on 447 cases of asthma was done in double- or single-blinded control method, and the therapeutic effect and safety of JXT were analysed. The effect of JXT in anti-asthma, expectorant, antisepsis, hypoxia tolerance was studied, and its acute and chronic toxicity was also examined.Results: The clinical control rate in patients treated with JXT was 38.2 %, the markedly effective rate 31.2% and the total effective rate 93.4 %, whereas in patients treated with Oleum Vitex Negundo, the respective rates were 13.3 %, 20.0 % and 68.5%. Results of pulmonary function and IgE measurement were consistent with clinical effect. Experimental study showed that JXT has the actions of anti-asthma, expectorant, antisepsis and hypoxia tolerance enhancement, with its LD50(40.48 ± 5.17) g/kg.Conclusion: JXT is a new Chinese herbal preparation of good effect, with less toxic-side effect for asthma treatment.Keywords：Juanxiao Tablet;bronchial asthma;asthma relieving;expectorant;bacteriostasis
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Published：2021-08-27 - Keywords：Ferulic Acid;PGI2;Benazepril;Indometacin;Sodium Ferulic
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Published：2021-08-27 - Abstract：Objective: To study the effect of Xiongshao capsule (XSC) on vascular remodeling in porcine coronary balloon injury model.Methods: Coronary artery restenosis model was established by oversized balloon injury at mid-region of the left anterior descending coronary artery. They were divided into 5 groups, untreated or treated with probucol, Xueguantong, low and high dose of XSC respectively, and compared. The effect of XSC on late lumen loss resulted from vascular remodeling was evaluated by quantitative histological method with combining histopathological analysis and coronary angiographie examination.Results: The (59 ± 20)% of late lumen loss was caused by vascular remodeling and (41 ± 20)% caused by intimai hyperplasia. Compared with the control group, all the treatment could significantly reduce the late lumen loss after balloon injury (P<0.05 orP < 0.01 ), both low and high dose of XSC could significantly reduce the late lumen loss caused by remodeling (P<0.05 and P<0.01 respectively).Conclusion: Vascular remodeling plays an important role in late lumen stenosis formation in coronary artery after balloon injury. XSC could significantly inhibit the pathological vascular remodeling, and thus reduce the late lumen loss and prevent the restenosis of the injured coronary artery.Keywords：Xiongshao capsule;angioplasty;restenosis;vascular remodeling
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Published：2021-08-27 - Abstract：Objective: To observe the effect of Zhenjian Granule (ZJG) in improving essential hypertension and insulin resistance (IR).Methods: The 52 patients of hypertension were randomly divided into the ZJG group (n=32) and the control group (n=20), treated with ZJG and captopril respectively for 4 weeks. The changes of blood pressure, glucose, insulin, insulin sensitivity index (ISI) and blood lipid in them before and after treatment were determined and compared with those in normal group (17 healthy subjects).Results: Fasting insulin, blood glucose, and insulin 2 hrs after meal in hypertension patients before treatment were significantly higher but ISI was significantly lower than those in the normal control, indicating the existence of insulin resistance. After treatment, not only blood pressure in patients obviously lowered, but also levels of fasting insulin, blood glucose, and insulin 2 hrs after meal obviously lowered, while ISI markedly raised. IR was improved in both groups, and blood lipid were also improved in the ZJG group.Conclusion: ZJG was effective in reducing blood pressure, also it had significant effect in improving metabolic disorder, particularly on IR in patients of essential hypertension.Keywords：insulin resistance;Captopril;Essential Hypertension;Balloon Angioplasty;Coronary Angioplasty
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Published：2021-08-27 - Abstract：Objective: To observe the effect of Yishou Tiaozhi tablet (YSTZT) on the metabolism of free radical in mice of aging model.Methods: Using the aging model induced by hypodermic injection of 5% Dglalactose in mice for forty days. The content of malondialdehyde(MDA) and activity of Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in serum, activity of monoamine oxidase B (MAO-B), Na+-K+-ATPase in brain were investigated after treatment.Results: Compared with the aging model group, it showed that YSTZT could obviously inhibit the increase of level of MDA in serum and the activities of MAO-B in brain; and enhance the activities of SOD, GSH-Px in serum, and the activities of Na+ -K+ -ATPase in brain. Furthermore, YSTZT could obviously elevate the tolerance to fatigue and hypoxia in mice.Conclusion: YSTZT could effectively improve the free radical metabolism of senile body, it is an ideal and effective medicine in preventing aging, which is also one of the experimental basis in proving the theory of ”Spleen and Kidney Deficiency complicated with Stasis and Phlegm causes aging”.Keywords：Yishou Tiaozhi tablet;aging model;free radical
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Published：2021-08-27 - Abstract：Objective: To investigate the effect of compound 962 capsule (abbre. as 962) on liver lipid peroxidation and indexes of thymus and spleen in aged rats.Methods: Rats were divided into young control, aged model, Piracetam, 962 middle dose (0.9 g/kg) and high dose (1.8 g/kg) groups. All test drugs were administrated for 1 month by gastrogavage. The liver lipid peroxidation was determined by thiobarbituric (TBA) method. The thymus index and spleen index were determined by weighing method.Results: 962 (middle and high dose) attenuated liver lipid peroxidation, increased the thymus index of aged rats. There was no effect on spleen index in all drug-treated groups.Conclusion: 962 could decrease liver lipid peroxidation and increase thymus index. It suggested that 962 might be beneficial in retarding aging process.Keywords：traditional Chinese compound;aging;liver lipid peroxidation;thymus index;spleen index
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Published：2021-08-27 - Abstract：Objective: To compare the preventive effect of treatment with different methods of traditional Chinese medicine(TCM) for myocardial ischemia caused by coronary artery ligation in diabetic rats.Methods:Animal models established by streptozotocin plus coronary artery ligation were treated by modified Taoren Chengqi Decoction (MTRCQD) and different combinations of its ingredients respectively, and the effects on ischemia area, occurrence of arrhythmia, T-wave in ECG and 2 hrs post-operational survival rate were observed and compared.Results: MTRCQD and different combinations of its ingredients could reduce the area of ischemia and elevate survival rate significantly (P<0.05–0.01), especially in the group treated with MTRCQD plus leech. Improvement of T-wave was shown in the groups treated with MTRCQD or with MTRCQD plus leech.Conclusion: TCM treatment of invigorating Qi, nourishing Yin, purging Heat and activating blood circulation is an effective treatment for diabetic coronary heart disease.Keywords：Traditional Chinese Medicine Treatment;coronary artery ligation;Myocardial ischemia;diabetic rats
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Published：2021-08-27 - Abstract：Objective: To explore the inhibitory pathway and the possible mechanism of panicoin on platelet activation.Methods: The effect of panicoin on platelet aggregation, thromboxane B2 release, P-selectin (GMP-140) count, levels of cAMP and cGMP induced by ADP, epinephrine, arachidonic acid or thrombin were observed.Results: Panicoin could inhibit significantly platelet aggregation induced by ADP, arachidonic acid and epinephrine in a dose-dependent manner, thrombin induced GMP-140 on platelet surface and TXB2 release, and increase the platelet cAMP level, but showed no effect on cGMP level.Conclusion: Panicoin shows a potent inhibition on platelet activation and antithrombotic effect. The mechanisms seems to be related with elevation of platelet cAMP.Keywords：Arachidonic Acid;Platelet Aggregation;platelet activation;cGMP Level;Antithrombotic Effect
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Published：2021-08-27 - Abstract：Objective: To investigate the effect of Huan-shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor-β2 (TGF-β2) in the remnant kidney of rats.Methods : Male Wistar rats, after 4 weeks of 5/6 nephrectomy, were divided into two groups at random, and treated with water and Huanshuai Recipe (HSR) respectively. Serum creatinine (SCr) and urinary protein of 24-hours were observed at beginning, 6th week and 12th week of the experiment, and FN and TGF-β2 were assayed by immunohistochemical technique and computer assisted image analysis as well.Results : Serum SCr and urinary protein of 24-hours were increased significantly in the animals after 5/6 nephrectomy. The increased parameters decreased in the HSR group after treatment, as compared with those in the control group, and the difference was significant (P < 0.05); Immunohistochemical measurement and computer assisted image analysis showed a marked suppression of FN and TGF-β2 in animals of the HSR group with significant difference from those in the control group (P<0.05).Conclusion : HSR not only plays the role of renal function protection, but could also alleviate the glomerular pathological injury. The effect on TGF-β2 and FN may be the mechanism of HSR in slowing down the progression of chronic renal failure.Keywords：Huan-shuai Recipes;5/6 nephrectomized rats;transforming growth factor-β2;extracellular matrix
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Published：2021-08-27 - Abstract：Objective: To explore the effective method of integrated Chinese and Western Medicine (TCM-WM) in treating hypertensive cerebral hemorrhage (HCH).Methods: Sixty-one HCH patients were randomly divided into group A and group B, 30 cases in each group, the former group was treated with stereotaxic drainage and orally taken Zhuyu Xiaozhong Mixture (ZYXZM), and the latter group with stereotaxic drainage alone.Results: The total effective rate of group A was 83. 7%, significantly higher than that of group B (60% ), P < 0.05. In comparing the effect in the two groups, group A was significantly better than group B ( P < 0. 05 ) in neurological function recovery, quality of life and hematoma absorption.Conclusion: ZYXZM combined with stereotaxic drainage is effective in treating HCH, it could promote neurological function recovery and improve quality of life in patients.Keywords：Transform Growth Factor Beta;Western Medicine;Total Effective Rate;Remnant Kidney;Form Growth Factor
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Published：2021-08-27 -
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- Keywords：Premature Ejaculation;Morbid Condition;Heat Sensation;Kidney Insufficiency;Chronic Nephritis
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Published：2021-08-27 - Keywords：Borneol;Licorice Root;Activate Blood Circulation;Tongue Coating;Peony Root
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Published：2021-08-27 - Keywords：Asthma;Respir Crit;Fenoterol;Total Effective Rate;Chinese Drug
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Published：2021-08-27 - Keywords：Nitric Oxide;Baicalin;acute cerebral infarction;Blood Stasis Syndrome;High Performance Liquid Chromato
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Published：2021-08-27 -
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