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      Vol. 28 ， Issue 6 ， 2022

        Original Article

      • Shao-hua ZHANG,Yu-long WANG,Chun-xia ZHANG,Chun-ping ZHANG,Peng XIAO,Qian-feng LI,Wei-rong LIANG,Xiao-hua PAN,Ming-chao ZHOU
        Vol. 28, Issue 6, Pages: 483-491(2022) DOI: 10.1007/s11655-021-3525-0
        Abstract：Objective:To evaluate the effects of interactive dynamic scalp acupuncture (IDSA), simple combination therapy (SCT), and traditional scalp acupuncture (TSA) on motor function and gait of the lower limbs in post-stroke hemiplegia patients.Methods:A total of 231 patients with post-stroke hemiplegia was randomly divided into IDSA (78 cases), SCT (78 cases), and TSA (75 cases) groups by a random number table. Scalp acupuncture (SA) and lower-limb robot training (LLRT) were both performed in the IDSA and SCT groups. The patients in the TSA group underwent SA and did not receive LLRT. The treatment was administered once daily and 6 times weekly for 8 continuous weeks, each session lasted for 30 min. The primary outcome measures included Fugl-Meyer Assessment of the Lower Extremity (FMA-LE), Berg Balance Scale (BBS), Modified Barthel Index (MBI), and 6-min walking test (6MWT). The secondary outcome measures included stride frequency (SF), stride length (SL), stride width (SW), affected side foot angle (ASFA), passive range of motion (PROM) of the affected hip (PROM-H), knee (PROM-K) and ankle (PROM-A) joints. The patients were evaluated before treatment, at 1- and 2-month treatment, and 1-, and 2-month follow-up visits, respectively. Adverse events during 2-month treatment were observed.Results:Nineteen patients withdrew from the trial, with 8 in the IDSA group, 5 in the SCT group, and 6 in the TSA group. The FMA-LE, BBS, 6MWT and MBI scores in the IDSA group were significantly increased after 8-week treatment and 2 follow-up visits compared with the SCT and TSA groups (P<0.05 or P<0.01). Compared with pre-treatment, the grade distribution of BBS and MBI scores in the 3 groups were significantly improved at 1, 2-month treatment and 2 follow-up visits (P<0.05 or P<0.01). The SF, PROM-H, PROM-K and PROM-A in the IDSA group was significantly increased compared with the SCT and TSA groups after 8-week of treatment (P<0.05 or P<0.01). Compared with the SCT group, ASFA of the IDSA group was significantly reduced after 8-week of treatment (P<0.05). SF, SL, PROM-K and PROM-A were significantly increased at the 2nd follow-up visit whereas the ASFA was significantly reduced in the IDSA group compared with the SCT groups at 1st follow-up visit (P<0.05 or P<0.01). The SF was significantly increased in the SCT group compared with the TSA group after 8-week treatment (P<0.05). Compared with the TSA group, PROM-K, PROM-A were significantly increased at the 2nd follow-up visit (P<0.05).Conclusions:The effects of IDSA on lower-limb motor function and walking ability of post-stroke patients were superior to SCT and TSA. The SCT was comparable to TSA treatment, and appeared to be superior in improving the motion range of the lower extremities. (Registration No. ChiCTR1900027206)  
        Keywords：interactive dynamic scalp acupuncture;stroke;dyskinesia;lower extremity motor ability;gait;self-care capability;randomized controlled clinical trial   
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        Published：2022-06-27
      • Yang GAO,Yan-ming XIE,Gui-qian WANG,Ye-feng CAI,Xiao-ming SHEN,De-xi ZHAO,Ying-zhen XIE,Yin ZHANG,Fan-xing MENG,Hai-qing YU,Jun-jie JIANG,Rui-Li WEI
        Vol. 28, Issue 6, Pages: 492-500(2022) DOI: 10.1007/s11655-022-3306-4
        Abstract：Objective:To delineate the onset and recurrence characteristics of noncardiogenic ischemic stroke patients in China.Methods:A prospective, multicenter and registry study was carried out in 2,558 patients at 7 representative clinical sub-centers during November 3, 2016 to February 17, 2019. A questionnaire was used to collect information of patients regarding CM syndromes and constitutions and associated risk factors. Additionally, stroke recurrence was defined as a primary outcome indicator.Results:A total of 327 (12.78 %) patients endured recurrence events, 1,681 (65.72%) patients were men, and the average age was 63.33±9.45 years. Totally 1,741 (68.06%) patients suffered first-ever ischemic stroke, 1,772 (69.27%) patients reported to have hypertension, and 1,640 (64.11%) of them reported dyslipidemia, 1,595 (62.35%) patients exhibited small-artery occlusion by The Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification. Specifically, 1,271 (49.69%) patients were considered as qi-deficient constitution, and 1,227 (47.97%) patients were determined as stagnant blood constitution. There were 1,303 (50.94%) patients diagnosed as blood stasis syndrome, 1,280 (50.04%) patients exhibited phlegm and dampness syndrome and 1,012 (39.56%) patients demonstrated qi deficiency syndrome. And 1,033 (40.38%) patients declared intracranial artery stenosis, and 478 (18.69%) patients reported carotid artery stenosis. The plaque in 1,508 (41.36%) patients were of mixed. Particularly, 41.09% of them demonstrated abnormal levels of glycated hemoglobin (HbA1c) levels.Conclusions:Recurrence in minor and small-artery stroke cannot be ignored. Hypertension, dyslipidemia, abnormal HbA1c, intracranial artery stenosis and carotid plaque were more common in stroke patients. Particularly, phlegm-dampness and blood stasis syndromes, as well as qi deficiency and blood stasis constitutions, were still the main manifestations of stroke. (Trial registration at ClinicalTrials.gov, No. NCT03174535)  
        Keywords：noncardiogenic ischemic stroke;Chinese medicine hospital;onset characteristics;recurrence   
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        Published：2022-06-27
      • Xue-qing HU,Yuan ZHOU,Jian CHEN,Yi-yu LU,Qi-long CHEN,Yi-yang HU,Shi-bing SU
        Vol. 28, Issue 6, Pages: 501-508(2022) DOI: 10.1007/s11655-021-3279-8
        Abstract：Objective:To explore the molecular bases of Chinese medicine (CM) syndrome classification in chronic hepatitis B (CHB) patients in terms of DNA methylation, transcription and cytokines.Methods:Genome-wide DNA methylation and 48 serum cytokines were detected in CHB patients (DNA methylation: 15 cases; serum cytokines: 62 cases) with different CM syndromes, including dampness and heat of Gan (Liver) and gallbladder (CHB1, DNA methylation: 5 cases, serum cytokines: 15 cases), Gan stagnation and Pi (Spleen) deficiency (CHB2, DNA methylation: 5 cases, serum cytokines: 15 cases), Gan and Shen (Kidney) yin deficiency (CHB3, DNA methylation: 5 cases, serum cytokines: 16 cases), CHB with hidden symptoms (HS, serum cytokines:16 cases) and healthy controls (DNA methylation: 6 cases). DNA methylation of a critical gene was further validated and its mRNA expression was detected on enlarged samples. Genome-wide DNA methylation was detected using Human Methylation 450K Assay and further verified using pyrosequencing. Cytokines and mRNA expression of gene were evaluated using multiplex biometric enzyme-linked immunosorbent assay (ELISA)-based immunoassay and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), respectively.Results:Totally 28,667 loci, covering 18,403 genes were differently methylated among CHB1, CHB2 and CHB3 (P<0.05 and |△β value| > 0.17). Further validation showed that compared with HS, the hg19 CHR6: 29691140 and its closely surrounded 2 CpG loci were demethylated and its mRNA expressions were significantly up-regulated in CHB1 (P<0.05). However, they remained unaltered in CHB2 (P>0.05). Levels of Interleukin (IL)-12 were higher in CHB3 and HS than that in CHB1 and CHB2 groups (P<0.05). Levels of macrophage inflammatory protein (MIP)-1α and MIP-1β were higher in CHB3 than other groups and leukemia inhibrtory factor level was higher in CHB1 and HS than CHB2 and CHB3 groups (P<0.05). IL-12, MIP-1α and MIP-1β concentrations were positively correlated with human leukocyte antigen F (HLA-F) mRNA expression (R2=0.238, P<0.05; R2=0.224, P<0.05; R2=0.447, P<0.01; respectively). Furthermore, combination of HLA-F mRNA and differential cytokines greatly improved the differentiating accuracy among CHB1, CHB2 and HS.Conclusions:Demethylation of CpG loci in 5' UTR of HLA-F may up-regulate its mRNA expression and HLA-F expression was associated with IL-12, MIP-1α and MIP-1β levels, indicating that HLA-F and the differential cytokines might jointly involve in the classification of CM syndromes in CHB. (Registration No. ChiCTR-RCS-13004001)  
        Keywords：cytokine;chronic hepatitis B;DNA methylation;human leukocyte antigen F;Chinese medicine syndrome   
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        Published：2022-06-27
      • Jing-jing TANG,Guang-xi LI,Zhi-guo LIU,Rong YI,Dong YU,Yue-bo ZHANG,Shuang-qiao ZHAO,Shi-han WANG
        Vol. 28, Issue 6, Pages: 509-517(2022) DOI: 10.1007/s11655-020-3255-8
        Abstract：Objective:To detect whether Danlou Tablet (DLT) regulates the hypoxia-induced factor (HIF)-1α- angiopoietin-like 4 (Angptl4) mRNA signaling pathway and explore the role of DLT in treating chronic intermittent hypoxia (CIH)-induced dyslipidemia and arteriosclerosis.Methods:The mature adipocytes were obtained from 3T3-L1 cell culturation and allocated into 8 groups including control groups (Groups 1 and 5, 0.1 mL of cell culture grade water); DLT groups (Groups 2 and 6, 0.1 mL of 1,000 μg/mL DLT submicron powder solution); dimethyloxalylglycine (DMOG) groups (Groups 3 and 7, DMOG and 0.1 mL of cell culture grade water); DMOG plus DLT groups (Groups 4 and 8, DMOG and 0.1 mL of 1,000 μg/mL DLT submicron powder solution). Groups 1–4 used mature adipocytes and groups 5–8 used HIF-1α-siRNA lentivirus-transfected mature adipocytes. After 24-h treatment, real-time polymerase chain reaction and Western blot were employed to determine the mRNA and protein expression levels of HIF-1α and Angptl4. In animal experiments, the CIH model in ApoE-/- mice was established. Sixteen mice were complete randomly divided into 4 groups including sham group, CIH model group [intermittent hypoxia and normal saline (2 mL/time) gavage once a day], Angptl4 Ab group [intermittent hypoxia and Angptl4 antibody (30 mg/kg) intraperitoneally injected every week], DLT group [intermittent hypoxia and DLT (250 mg/kg) once a day], 4 mice in each group. After 4-week treatment, enzyme linked immunosorbent assay was used to detect the levels of serum total cholesterol (TC) and triglyceride (TG). Hematoxylin-eosin and CD68 staining were used to observe the morphological properties of arterial plaques.Results:Angptl4 expression was dependent on HIF-1α, with a reduction in mRNA expression and no response in protein level to DMOG or DLT treatment in relation to siHIF-1α-transfected cells. DLT inhibited HIF-1α and Angptl4 mRNA expression (P<0.05 or P<0.01) and reduced HIF-1α and Angptl4 protein expressions with DMOG in mature adipocytes (all P<0.01), as the effect on HIF-1α protein also existed in the presence of siHIF-1α (P<0.01). ApoE-/- mice treated with CIH had increased TG and TC levels (all P<0.01) and atherosclerotic plaque. Angptl4 antibody and DLT both reduced TG and TC levels (all P<0.01), as well as reducing atherosclerotic plaque areas, narrowing arterial wall thickness and alleviating atherosclerotic lesion symptoms to some extent.Conclusion:DLT had positive effects in improving dyslipidemia and arteriosclerosis by inhibiting Angptl4 protein level through HIF-1α-Angptl4 mRNA signaling pathway.  
        Keywords：Danlou Tablet;Chinese medicine;hypoxia-induced factor;angiopoietin-like 4;dyslipidemia;arteriosclerosis   
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        Published：2022-06-27
      • Lei HAN,Ping ZHAO,Xue HAN,Jie WEI,Fei WANG,Yi LI,Guang-jin GUO
        Vol. 28, Issue 6, Pages: 518-530(2022) DOI: 10.1007/s11655-021-3276-y
        Abstract：Objective:To compare the analgesic effects of two types of spinal manipulation (SM) in acute lumbar radiculopathy (ALR) model rats induced by self-transplantation of autologous nucleus pulposus (ANP), and clarify the therapeutic mechanism.Methods:Totally 108 male Sprague-Dawley rats were randomly divided into 6 groups by a random number table (18 rats in each group), including a blank group with no interference, a sham operation group with a surgery by making a local soft tissue incision on the left side of L5–6 vertebral segment, a model group with ALR of L5 extraforaminal nerve by ANP self-transplantation without other interference, a sham manipulation (SMA) group with simulating physical rotation, as well as a mobilization (MOB) group with simulating low-velocity and variable-amplitude rotation and a manipulation (MAN) group with simulating high-velocity and low-amplitude rotation. The interventions in SMA, MOB, and MAN groups started 1 day after modeling followed by another 5 treatments at days 3, 5, 8, 10 and 12. Rats in the other 3 groups did not receive any special intervention. Behavioral pain tests of 50% mechanical pain withdrawal threshold (50% PWT) and paw withdrawal latency (PWL) were conducted 1 day before operation followed by another 10 tests on days 1–7, 10, 12 and 14. Immunohistochemical expression of nitric oxide synthase (NOS) was investigated on days 5 and 12 after operation.Results:After 3 experimental SM interventions, 50% PWT and PWL were higher in the MAN group than the SMA group on days 6 and 7, and higher on days 10, 12 and 14 postoperatively (P<0.05 or P<0.01), while the same indices were significantly higher in the MOB group than MAN group on days 1–4 (P<0.05 or P<0.01). The expression of NOS was lower in the MAN and MOB groups than SMA group on day 12 postoperatively (P<0.01).Conclusions:Both manipulation and mobilization produced better results than sham interference in relieving pain by reducing neuroinflammation possibly. At the early period, compared with manipulation, mobilization presented less sensitive response to pain until later visit. SM may inhibit the overexpression of NOS, thereby alleviating severe radiculopathy.  
        Keywords：spinal manipulation;radiculopathy;anti-inflammation;analgesia   
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        Published：2022-06-27
      • Chun-lei ZHENG,Ke-zuo HOU,An-qi WANG,Wan-xia FANG,Shi-tong YU,Jin-e LIANG,Hai-yan QI,Xiu-juan QU,Yun-peng LIU,Xiao-fang CHE
        Vol. 28, Issue 6, Pages: 524-530(2022) DOI: 10.1007/s11655-020-3265-6
        Abstract：Objective:To explore the mechanisms underlying the proliferative inhibition of Chinese herbal medicine Kang-Ai injection (KAI) in gastric cancer cells.Methods:Gastric cancer cell lines MGC803 and BGC823 were treated by 0, 0.3%, 1%, 3% and 10% KAI for 24, 48 and 72 h, respectively. The cell proliferation was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The apoptosis and cell cycle were evaluated by flow cytometry. Interleukin (IL)-6 mRNA and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immune sorbent assay (ELISA), respectively. The protein expression levels of cyclin A, cyclin E, cyclin B1, cyclin D1, p21, retinoblastoma (RB), protein kinase B (AKT), extracellular regulated protein kinases (ERK), signal transducer and activator of transcription (STAT) 1 and STAT3 were detected by Western blot.Results:KAI inhibited the proliferation of MGC803 and BGC823 gastric cancer cells in dose- and time-dependent manner. After treated with KAI for 48 h, the proportion of G1 phase was increased, expression level of cyclin D1 and phosphorylation-RB were down-regulated, whereas the expression of p21 was up-regulated (all P<0.01). Furthermore, 48-h treatment with KAI decreased the phosphorylation level of STAT3, inhibited the mRNA and protein expressions of IL-6 (all P<0.01). IL-6 at dose of 10 ng/mL significantly attenuated the proliferative effect of both 3% and 10% KAI, and recovered KAI-inhibited STAT3 phosphorylation and cyclin D1 expression level (all P<0.01).Conclusion:KAI exerted an anti-proliferative function by inhibiting IL-6/STAT3 signaling pathway followed by the induction of G1 phase arrest in gastric cancer cells.  
        Keywords：Kang-Ai Injection;gastric cancer;G1 arrest;interleukin-6;signal transducer and activator of transcription 3;Chinese herbal medicine   
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        Published：2022-06-27
      • Baran Ghezelbash,Nader Shahrokhi,Mohammad Khaksari,Gholamreza Asadikaram,Maryam Shahrokhi,Sara Shirazpour
        Vol. 28, Issue 6, Pages: 531-537(2022) DOI: 10.1007/s11655-022-3307-3
        Abstract：Objective:To evaluate the effect of Shilajit, a medicine of Ayurveda, on the serum changes in cytokines and adipokines caused by non-alcoholic fatty liver disease (NAFLD).Methods:After establishing fatty liver models by feeding a high-fat diet (HFD) for 12 weeks, 35 Wistar male rats were randomly divided into 5 groups, including control (standard diet), Veh (HFD + vehicle), high-dose Shilajit [H-Sh, HFD + 250 mg/(kg•d) Shilajit], low-dose Shilajit [L-Sh, HFD + 150 mg/(kg•d) Shilajit], and pioglitazone [HFD + 10 mg/(kg•d) pioglitazone] groups, 7 rats in each group. After 2-week of gavage administration, serum levels of glucose, insulin, interleukin 1beta (IL-1β), IL-6, IL-10, tumor necrosis factor-alpha (TNF-α), adiponectin, and resistin were measured, and insulin resistance index (HOMA-IR) was calculated.Results:After NAFLD induction, the serum level of IL-10 significantly increased and serum IL-1β, TNF-α levels significantly decreased by injection of both doses of Shilajit and pioglitazone (P<0.05). Increases in serum glucose level and homeostasis model of HOMA-IR were reduced by L-Sh and H-Sh treatment in NAFLD rats (P<0.05). Both doses of Shilajit increased adiponectin and decreased serum resistin levels (P<0.05).Conclusion:The probable protective role of Shilajit in NAFLD model rats may be via modulating the serum levels of IL-1β, TNF-α, IL-10, adipokine and resistin, and reducing of HOMA-IR.  
        Keywords：non-alcoholic fatty liver disease;Shilajit;adipokines;cytokines;glucose;insulin;dietary supplement;Ayurvedic medicine   
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        Published：2022-06-27
      • Xin SUN,Ye TAN,Jing LYU,Hong-liang LIU,Zhi-min ZHAO,Cheng-hai LIU
        Vol. 28, Issue 6, Pages: 538-544(2022) DOI: 10.1007/s11655-021-3293-x
        Abstract：Objective:To screen the active components from Fuzheng Huayu Recipe (FZHY) and redesign a new recipe composed of the active components, and validate the effect of active components formulation from FZHY against liver fibrosis.Methods:Thirty-two components from FZHY were evaluated for their activities against liver fibrosis respectively, with 6 kinds of cell models in vitro, including oxidative stressed hepatocyte in L-02, hypoxia injured/proliferative hepatic sinusoidal endothelial cells in SK-HEP-1 and human hepatic sinusoidal endothelial cells (HHSEC), and activated hepatic stellate cell in LX-2. The comprehensive activity of each component against liver fibrosis was scored according to the role of original herbs in FZHY and cell functions in fibrogenesis. Totally 7 active components were selected and combined with equal proportion to form a novel active components formulation (ACF). The efficacy of ACF on liver fibrosis were evaluated on activation of LX-2 and proliferation of HHSEC in vitro and in liver fibrosis model mice induced by dimethylnitrosamine (DMN). Totally 72 mice were divided into 6 groups using a random number table, including normal, high-dose ACF control (20 μmol/L×7 components/kg body weight), model, low-, medium-, high-dose ACF groups (5, 10, 20 μmol/L×7 components/kg body weight, respectively). Hematoxylin eosin and Sirius red stainings were used to observe inflammation and fibrosis change of liver tissue; scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were utilized to observe the effect of ACF on ultrastructure of hepatic sinusoids.Results:Fifteen components from FZHY showed higher scores for their activity on against liver fibrosis. Among them, 7 components including tanshinone ⅡA, salvianolic acid B, cordycepin, amygdalin, quercetin, protopanaxatriol, and schizandrin B were recombined with equal proportions to form ACF. ACF at 1, 2, 4 μmol/L showed strong inhibitory effects on activation of LX-2 and proliferation of HHSEC in vitro (all P<0.01). Compared with the model group, ACF attenuated liver collagen deposition, improved sinusoidal capillarization in a dose-dependent manner (all P<0.05).Conclusions:ACF exerts a satisfactory effect against experimental liver fibrosis and attenuates sinusoidal capillarization, which warrant a further research and development for herbal components formulation on liver fibrosis.  
        Keywords：active components formulation;Fuzheng Huayu Recipe;Chinese medicine;liver fibrosis;hepatic stellate cells;hepatic sinusoidal capillarization   
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        Published：2022-06-27

        Case Report

      • Ting-ting HE,Xiao-he XIAO,Jia-bo WANG
        Vol. 28, Issue 6, Pages: 545-546(2022) DOI: 10.1007/s11655-021-3528-x
        Abstract：报道1例服用含栀子中药后出现栀子蓝色素沉积现象的临床病例. 通过观察患者服用含栀子中药前后及停药后的色素沉积变化情况, 收集患者一般临床资料、基础疾病情况、肝肾功能等实验室指标以及不同节点相片. 应用ImageJ软件测定眼周色素沉积的平均光密度值进行比对分析. 结果发现, 患者服用含栀子中药2个月后眼周皮肤出现明显的栀子蓝色素沉积, 即 "栀子蓝面容" , 停药3个月后色素沉积现象逐渐消失. 虽然尚无栀子色素导致严重毒副作用的病例报道, 但不建议长期服用栀子或栀子色素. 当观察到 "栀子蓝面容" 时建议停止继续用药, 警惕体内栀子色素过量带来蓄积风险.  
          
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        Published：2022-06-27

        Literature Research

      • Tie-xin ZENG,Jin PEI,Yu-jing MIAO,Yan ZHENG,Sheng-jiu GU,Lei ZHAO,Lin-fang HUANG
        Vol. 28, Issue 6, Pages: 547-553(2022) DOI: 10.1007/s11655-021-3315-8
        Abstract：Objective:To investigate the current status and further development of Panax genus and 6 important individual species including P. notoginseng, P. quinquefolium, P. vietnamensis, P. japonicus, P. stipuleanatus and P. zingiberensis.Methods:The bibliometric analysis was based on the Web of Science core database platform from Thomson Reuters. Totally, 7,574 records of scientific research of Panax species published from 1900—2019 were analyzed. The statistical and visualization analysis was performed by CiteSpace and HistCite software.Results:The academic research of Panax species increase promptly. Plant science is the main research field while research and experimental medicine and agricultural engineering will be the further development tendency. Particularly, the discrimination research of P. notoginseng will be the research tendency among Panax species, especially diversity research. In addition, P. vietnamensis deserves more attention in the genus Panax.Conclusion:This research provides a reference for further research of the genus and individual species.  
        Keywords：Panax;development tendency;bibliometric;Ginseng discrimination;cultivation engineering   
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        Thinking and Methodology

      • Xu-dong TANG,Xiang-xue MA
        Vol. 28, Issue 6, Pages: 554-559(2022) DOI: 10.1007/s11655-022-3534-7
        Abstract：Functional gastrointestinal disorders (FGIDs) are common disorders that are characterized by persistent and recurring gastrointestinal symptoms. Many patients with FGIDs have overlapping symptoms, which impaired the quality of life and ability to work of patients, and left a considerable impact on health-care systems and society. Chinese medicines (CMs) are commonly utilized by many patients with FGIDs. This article discusses the current status of diagnosis and treatment of FGIDs, the advantages and characteristics of CM treatment, and how integrated medicine can make a breakthrough in FGIDs diagnosis and treatment.  
        Keywords：functional gastrointestinal disorders;overlapping symptoms;integrative medicine   
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        Published：2022-06-27

        Academic Exploration

      • Feng-lin SHEN,Yan-na ZHAO,Xiao-ling YU,Bo-lin WANG,Xiao-long WU,Gao-chen LAN,Rui-lan GAO
        Vol. 28, Issue 6, Pages: 560-566(2022) DOI: 10.1007/s11655-021-3316-7
        Abstract：Aberrant regulation of DNA methylation plays a crucial causative role in haematological malignancies (HMs). Targeted therapy, aiming for DNA methylation, is an effective mainstay of modern medicine; however, many issues remain to be addressed. The progress of epigenetic studies and the proposed theory of "state-target medicine" have provided conditions to form a new treatment paradigm that combines the "body state adjustment" of CM with targeted therapy. We discussed the correlation between Chinese medicine (CM) syndromes/states and DNA methylation in this paper. Additionally, the latest research findings on the intervention and regulation of DNA methylation in HMs, including the core targets, therapy status, CM compounds and active components of the Chinese materia medica were concisely summarized to establish a theoretical foundation of "state-target synchronous conditioning" pattern of integrative medicine for HMs, simultaneously leading a new perspective in clinical diagnosis and therapy.  
        Keywords：haematological malignancies;DNA methylation;Chinese medicine;state-target medicine   
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        Published：2022-06-27

        Review

      • Xue GONG,Min YANG,Chun-nian HE,Ya-qiong BI,Chun-hong ZHANG,Min-hui LI,Pei-gen XIAO
        Vol. 28, Issue 6, Pages: 567-574(2022) DOI: 10.1007/s11655-020-3270-9
        Abstract：Medicinal plants have provided numerous medicinal active ingredients for thousands of years and these ingredients have been used in Chinese medicine (CM) and traditional pharmacologies worldwide. Recently, the exploitation and utilisation of medicinal plant resources has increased significantly. The results of the studies have led to the identification of many active components, such as steroidal alkaloids, saponins, terpenoids, and glycosides, in various medicinal plants with different evolutionary levels. Moreover, research on the chemical classification, molecular phylogeny, and pharmacological activity of medicinal plants is increasing in popularity. Pharmacophylogeny is an interdisciplinary topic that studies the correlation between plant phylogeny, chemical composition, and curative effects (pharmacological activity and the traditional curative effect) of medicinal plants. In addition, it provides the basic tools to enable research and development of CM resources. This literature review, based on the genetic relationship between phytogroup and species, highlights the formation process, research content, applications, and future directions of pharmacophylogeny.  
        Keywords：pharmacophylogeny;phytogroup;molecular phylogeny;chemical composition;curative effect   
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