Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride

Cao Hong-xin; Sun Hui; Jiang Xin-gang; Lu Hai-tao; Zhang Guang-mei; Wang Xi-jun; Sun Wen-jun; Wu Ze-ming; Wang Ping; Liu Lian; Zhou Jue

doi:10.1007/s11655-009-0204-y

Your Location：

Home >

Browse articles >

Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride

OriginalPaper | Updated：2021-08-27

- Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride
- Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride
- 中国结合医学杂志（英文版） 2009年15卷第3期页码：204-209
- Affiliations：
  
  1. Department of Pharmacognosy, Heilongjiang University of Chinese Medicine,Harbin,China
  2. China Academy of Chinese Medical Sciences,Beijing,China
  3. First Hospital, Harbin Medical University,Harbin,China
- Author bio：
- Funds：
  
  Supported by the Major State Basic Research Development Program of China (973 Program, No. 2005CB523406)
- DOI：10.1007/s11655-009-0204-y
  中图分类号：
- 纸质出版日期：2009，
  
  网络出版日期：2009-7-2，
Scan for full text
Hong-xin, C., Hui, S., Xin-gang, J. et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride., Chin. J. Integr. Med. 15, 204 (2009). https://doi.org/10.1007/s11655-009-0204-y

Cao Hong-xin, Sun Hui, Jiang Xin-gang, et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride[J]. Chinese Journal of Integrative Medicine, 2009,15(3):204-209.
Hong-xin, C., Hui, S., Xin-gang, J. et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride., Chin. J. Integr. Med. 15, 204 (2009). https://doi.org/10.1007/s11655-009-0204-y DOI：

Cao Hong-xin, Sun Hui, Jiang Xin-gang, et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride[J]. Chinese Journal of Integrative Medicine, 2009,15(3):204-209. DOI： 10.1007/s11655-009-0204-y.

摘要

To optimize the animal model of liver injury that can properly represent the pathological characteristics of dampness-heat jaundice syndrome of traditional Chinese medicine. The liver injury in the model rat was induced by α-naphthylisothiocyanate (ANIT) and carbon tetrachloride (CCl4 ) respectively

and the effects of Yinchenhao Decoction (茵陈蒿汤

YCHD)

a proved effective Chinese medical formula for treating the dampness-heat jaundice syndrome in clinic

on the two liver injury models were evaluated by analyzing the serum level of alanine aminotransferase (ALT)

asparate aminotransferase (AST)

alkaline phosphatase (ALP)

malondialchehyche (MDA)

total bilirubin (T-BIL)

superoxide dismutase (SOD)

glutathione peroxidase (GSH-PX) as well as the ratio of liver weight to body weight. The experimental data were analyzed by principal component analytical method of pattern recognition. The ratio of liver weight to body weight was significantly elevated in the ANIT and CCl4 groups when compared with that in the normal control (P<0.01). The contents of ALT and T-BIL were significantly higher in the ANIT group than in the normal control (P<0.05

P<0.01)

and the levels of AST

ALT and ALP were significantly elevated in CCl4 group relative to those in the normal control P<0.01). In the YCHD group

the increase in AST

ALT and ALP levels was significantly reduced (P<0.05

P<0.01)

but with no significant increase in serum T-BIL. In the CCl4 intoxicated group

the MDA content was significantly increased and SOD

GSH-PX activities decreased significantly compared with those in the normal control group

respectively (P<0.01). The increase in MDA induced by CCl4 was significantly reduced by YCHD P<0.05). YCHD showed significant effects on preventing liver injury progression induced by CCl4

and the closest or most suitable animal model for damp-heat jaundice syndrome may be the one induced by CCl4.

Abstract

and the effects of Yinchenhao Decoction (茵陈蒿汤

YCHD)

a proved effective Chinese medical formula for treating the dampness-heat jaundice syndrome in clinic

on the two liver injury models were evaluated by analyzing the serum level of alanine aminotransferase (ALT)

asparate aminotransferase (AST)

alkaline phosphatase (ALP)

malondialchehyche (MDA)

total bilirubin (T-BIL)

superoxide dismutase (SOD)

P<0.01)

and the levels of AST

ALT and ALP were significantly elevated in CCl4 group relative to those in the normal control P<0.01). In the YCHD group

the increase in AST

ALT and ALP levels was significantly reduced (P<0.05

P<0.01)

but with no significant increase in serum T-BIL. In the CCl4 intoxicated group

the MDA content was significantly increased and SOD

GSH-PX activities decreased significantly compared with those in the normal control group

respectively (P<0.01). The increase in MDA induced by CCl4 was significantly reduced by YCHD P<0.05). YCHD showed significant effects on preventing liver injury progression induced by CCl4

and the closest or most suitable animal model for damp-heat jaundice syndrome may be the one induced by CCl4.

关键词

Yinchenhao Decoctionα-naphthylisothiocyanatecarbon tetrachlorideliver injurydampness-heat jaundice syndrome

Keywords

Yinchenhao Decoctionα-naphthylisothiocyanatecarbon tetrachlorideliver injurydampness-heat jaundice syndrome

references

Yang LL, Yen KY, Amagaya S, Ogihara Y. Systematic analysis of some Chinese medicinal prescriptions. 1. Antihepatotoxic principles of Ryutan-shakan-to. Med Pharmaceutical Soc 1990;7:28–34.

Liu SJ, Zhou SW. 6,7-dimethoxycoumarin attenuated cisplatin-induced DNA interstrand crosslink and DN-Aprotein crosslink in primary cultured rabbit kidney proximal tubular cells. Acta Pharmacol Sin 1987;20:391–394.

Wang T. Clandestine essentials from imperial library. Shanghai: Shanghai Ancient Books Publishing House; 1987:1.

Wang LQ, Miao LC, Wang XJ. Study on current progress and clinical application of Inchinko-To. Chin J Chin Mate Med (Chin) 2001;8:23–25.

Wang XJ, Li TL, Sun H. Hepatoprotective effects of Yinchenhao Tang and its constituent absorbed into blood after oral administration. Chin Pharmacol Bull (Chin) 2004;20: 239–240.

Nomura M. Cooperative effect of Yinchenhaotang on ANIT. J Tradit Chin Med (Engl Ed) 1997;19:40.

Yoshikawa M. Inhibitory effects of coumarin and acetylene constituents from the roots of Angelica furcijuga on D-galactosamine/lipopolysaccharide induced liver injury in mice and on nitric oxide production in lipopolysaccharide-activated mouse peritoneal macrophages. Bioorg Med Chem 2006;14:456–463.

Yamashiki M, Mase A, Arai I, Huang XX, Nobori T, Nishimura A, et al. Effects of the Japanese herbal medicine “Inchinko-to” (TJ2135) on concanavalin A2 induced hepatitis in mice. Clin Sci (Lond) 2000;99:421–431.

Rui J, Wu YM, Tang YT. Study on the pharmacological action of Yin-chen compound capsule. Tianjin Pharmacy (Chin) 2004;16:6–9.

Xu MJ, Liu YN, Ge YJ, Jing MW, Guo M. Time effect of carbon tetrachloride on the liver damage in rats. Mod Prev Med (Chin) 2005; 32:1456–1457.

Moldeus P, Hogberg J, Orrenius S. Isolation and use of liver cells. Meth Enzymol 1978;1978:60–71.

IFCC. Methods for the measurement of catalytic concentration of enzymes. J Clin Chem Clin Biochem (Chin) 1986;24:481–495.

Hochstein P, Utley H. Hydrogen peroxide detoxication by glutathione peroxidase and catalase in rat liver homogenates. Mol Pharmacol 1968;8:574–579.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;142:290–296.

Oynagui Y. Reevaluation of assay methods and establishment of kit for superoxide dismutase. Anal. Biochem 1984;142:290–296.

Plaa GL, Priestly BG. Intrahepatic cholestasis induced by drugs and chemicals. Pharmacol Rev 1977; 28: 207–273.

Roth RA, Dahm LJ. Neutrophil- and glutathione-mediated hepatotoxicity of α-naphthylisothiocyanate. Drug Metab Rev 1997;29:153–165.

Dahm LJ, Schultze AE, Roth RA. An antibody of neutrophils attenuates α-naphthylisothiocyanate induced liver injury. J Pharmacol Exp Ther 1991;256:412–420.

Hill DA, Roth RA. α-Naphthylisothiocyanate causes neutrophils to release factors that are cyotoxic to hepatocytes. Pharmacol Toxicol 1998;148:169–175.

Hill DA, Jean PA, Roth RA. Bile duct epithelial cells exposed to alpha-naphthylisothiocyanate produces a factor that causes neutrophil-dependent hepatocellular injury in vitro. J Toxicol Sci 1999;47:118–125.

Kongo M, Ohta Y, Nishida K, Sasaki E, Harada N, Ishiguro I. An association between lipid peroxidation and α-naphthylisothiocyanate induced liver injury in rats. Toxicol Lett 1999;105:103–110.

Aleynik SI, Leo MA, Ma X, Aleynik MK, Lieber CS. Polyenyl phosphatidylcholine prevents carbon tetrachloride-induced lipid peroxideation while it attenuates liver fibrosis. Hepatol 1997;27: 554–561.

Lee JY, Lee SH, Kim HJ, Ha JM, Lee SH, Lee JH, et al. The preventive inhibition of chondroitin sulfate against the CCl4-induced oxidative stress of subcellular level. Pharmacol Res 2004;27:340–345.

Basu, S. Carbon tetrachloride-induced lipid peroxidation: eicosanoid formation and their regulation by antioxidant nutrients. Toxicol 2003;189:113–127.

Li PS, Liu DZ. Treaties on exogenous febrile diseases. Beijing: People’s Medical Publishing House;1987:328.

Sakaida I, Tsuchiya M, Kawaguchi K, Kimura T, Terai S, Okita K. Herbal medicine Inchin-ko-to (TJ-135) prevents liver fibrosis and enzyme-altered lesions in rat liver cirrhosis induced by a choline-deficient L-amino acid-defined diet. Hepatol 2003;38:762–769.

Imanishi Y, Maeda N, Otogawa K, Seki S, Matsui H, Kawada N, et al. Herb medicine Inchin-ko-to (TJ-135) regulates PDGF-BB-dependent signaling pathways of hepatic stellate cells in primary culture and attenuates development of liver fibrosis induced by thioacetamide administration in rats. J Herpetol 2004;41:242–250.

Inao M, Mochida S, Matsui A, Eguchi Y, Yulutuz Y, Wan Y, et al. Japanese herbal medicine Inchin-ko-to as a therapeutic drug for liver fibrosis. Hepatol 2004;10:584–591.

Moridani MY, Scobie H, Jamshidzadeh A, Salehi P, O’Brien PJ. Caffeic acid, chlorogenic acid, and dihydrocaffeic acid metabolism: glutathione conjugate formation. Drug Metab Dispos 2001;29:1432–1439.

Chang YC, Chou FP, Huang HP, Hsu JD, Wang CJ. Inhibition of cell cycle progression by penta-acetyl geniposide in rat C6 glioma cells. Toxicol Appl Pharmacol 2004;198:11–20.

Kano Y, Wang XJ, Shirakawa J, Maeda R. Pharmacological properties of galenical prepration (XXH) pharmacokinetics study of 6,7-dimethylesculetin in rats. J Tradit Chin Med (Chin) 1994;11:176–180.

Guo MZ, Xu HR, Li XS. Effect of rhein on hepatic damage. Chin J Dig Dis (Chin) 2002;18: 37–38.

FULL TEXT LINK

More>

浏览量

359

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Changes of pharmacokinetics of 6,7-dimethoxycoumarin in a rat model of alpha-naphthylisothiocyanate-induced experimental hepatic injury after Yinchenhao Decoction (茵陈蒿汤) treatment

Intervention effects of Jianpi Liqi Huoxue Decoction (健脾理气活血汤) on lipid peroxidative liver injury induced by alcohol

Clinical study on Ganbi decoction in treating antituberculotic agent-caused liver injury

Effect of magnesium lithosperamate B on D-galactosamine induced liver injury in rats