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Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model
Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model
- 中国结合医学杂志（英文版） 2009年15卷第4期 页码：284-288
- Affiliations：
1. Department of Endocrinology, Provincial Hospital Affiliated to Shandong University,Jinan,China
2. Department of Endocrinology of Zaozhuang City Hospital,Shandong,China
3. Scientific Centre, Shandong Provincial Hospital Affiliated to Shandong University,Jinan,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 30672748), the Natural Science Foundation of Shandong Province (No. 22003C02), and the Shandong Administration of Traditional Chinese Medicine (No. 2005-070)
DOI：10.1007/s11655-009-0284-8
中图分类号：纸质出版日期：2009，
网络出版日期：2009-8-18，
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Han, Y., Zhou, J., Yu, Sj. et al. Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model., Chin. J. Integr. Med. 15, 284–288 (2009). https://doi.org/10.1007/s11655-009-0284-8
Yong Han, Jing Zhou, Shu-jing Yu, et al. Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model[J]. Chinese Journal of Integrative Medicine, 2009,15(4):284-288.
Han, Y., Zhou, J., Yu, Sj. et al. Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model., Chin. J. Integr. Med. 15, 284–288 (2009). https://doi.org/10.1007/s11655-009-0284-8 DOI：
Yong Han, Jing Zhou, Shu-jing Yu, et al. Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model[J]. Chinese Journal of Integrative Medicine, 2009,15(4):284-288. DOI： 10.1007/s11655-009-0284-8.
摘要
To investigate the inhibitory effects of Kangjia Pill (抗甲丸
KJP) on the cell proliferation in rat goiter model induced by methimazole (MMI). Fifty-six Wistar rats were randomly divided into four groups: the normal group
MMI model group (MMI)
low dose of KJP group (LKJP)
and high dose of KJP (HKJP). Except the normal group (20 rats)
the other groups (12 rats in each) were given 0.04% (w/v) MMI through the drinking water until the end of the experiment. One week later
the rats in the LKJP and HKJP groups were given KJP by gastrogavage at the dose of 250 mg/(kg · d) and 1 000 mg/(kg · d)
respectively for 12 weeks. The relative thyroid weight (mg/100 g body weight) of each rat was accessed. The expression of proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry
and the correlation analysis between the PCNA positive thyrocytes and the relative thyroid weight was performed. The expressions of PCNA and cyclin D1 were examined with Western blotting. After KJP treatment for 12 weeks
compared with the MMI group
the relative thyroid weight of the HKJP group decreased significantly
and the positive thyrocyte populations of PCNA in the two KJP groups reduced markedly (all P<0.05). The correlation analysis showed that PCNA was closely correlated with thyrocyte proliferation (r=0.685
P<0.05). KJP significantly decreased the protein expression of PCNA and cyclin D1 in the thyroid specimens (P<0.05)
the high dose showed better effects. KJP played a therapeutic role via inhibiting cell proliferation in the rat goitrous glands.
Abstract
To investigate the inhibitory effects of Kangjia Pill (抗甲丸
KJP) on the cell proliferation in rat goiter model induced by methimazole (MMI). Fifty-six Wistar rats were randomly divided into four groups: the normal group
MMI model group (MMI)
low dose of KJP group (LKJP)
and high dose of KJP (HKJP). Except the normal group (20 rats)
the other groups (12 rats in each) were given 0.04% (w/v) MMI through the drinking water until the end of the experiment. One week later
the rats in the LKJP and HKJP groups were given KJP by gastrogavage at the dose of 250 mg/(kg · d) and 1 000 mg/(kg · d)
respectively for 12 weeks. The relative thyroid weight (mg/100 g body weight) of each rat was accessed. The expression of proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry
and the correlation analysis between the PCNA positive thyrocytes and the relative thyroid weight was performed. The expressions of PCNA and cyclin D1 were examined with Western blotting. After KJP treatment for 12 weeks
compared with the MMI group
the relative thyroid weight of the HKJP group decreased significantly
and the positive thyrocyte populations of PCNA in the two KJP groups reduced markedly (all P<0.05). The correlation analysis showed that PCNA was closely correlated with thyrocyte proliferation (r=0.685
P<0.05). KJP significantly decreased the protein expression of PCNA and cyclin D1 in the thyroid specimens (P<0.05)
the high dose showed better effects. KJP played a therapeutic role via inhibiting cell proliferation in the rat goitrous glands.
关键词
goiterChinese Medicinecell proliferationproliferating cell nuclear antigencyclin D1
Keywords
goiterChinese Medicinecell proliferationproliferating cell nuclear antigencyclin D1
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