FOLLOWUS
Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats
Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats
- 中国结合医学杂志（英文版） 2009年15卷第6期 页码：435-441
- Affiliations：
1. Shuguang Hospital, Liver Disease Institute, Shanghai University of Traditional Chinese Medicine,Shanghai,China
2. The Experimental Center of Science and Technology, Shanghai University of Traditional Chinese Medicine,Shanghai,China
3. Shanghai Traditional Chinese Medicine Internal Medicine E-Institute, Key Lab of Liver & Kidney Diseases, Shanghai University of Traditional Chinese Medicine, Minister of Education,Shanghai,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 30772869), Major State Basic Research Development of China (973 Program, No, 2006CB504801) Shanghai Subject Chief Scientist (No. 08XD1404100), E-institute of Shanghai Municipal Education Commission (No. 03008), Innovative Research Team in Shanghai Municipal Education Commission
DOI：10.1007/s11655-009-0435-y
中图分类号：纸质出版日期：2009，
网络出版日期：2010-1-18，
Scan for full text
Chen, Y., Chen, Q., Lu, J. et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats., Chin. J. Integr. Med. 15, 435–441 (2009). https://doi.org/10.1007/s11655-009-0435-y
Yuan Chen, Qian Chen, Jing Lu, et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats[J]. Chinese Journal of Integrative Medicine, 2009,15(6):435-441.
Chen, Y., Chen, Q., Lu, J. et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats., Chin. J. Integr. Med. 15, 435–441 (2009). https://doi.org/10.1007/s11655-009-0435-y DOI：
Yuan Chen, Qian Chen, Jing Lu, et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats[J]. Chinese Journal of Integrative Medicine, 2009,15(6):435-441. DOI： 10.1007/s11655-009-0435-y.
摘要
To explore the mechanism of Danggui Buxue Decoction (当归补血汤
DBD) on the liver fifibrosis related to hepatic lipid peroxidation and matrix metalloproteinases (MMP) −2/9 activities. The liver fifibrosis in 28 rats was induced by an injection of carbon tetrachloride (CCl4) and fed with high lipid and low protein diet for 6 weeks
the model rats were randomly divided into the model group and DBD treated group
14 in each group
and another 10 rats as the normal group were observed as well. Rats in the DBD group were administered with DBD at the dose of 6 g/kg body weight for 6 weeks since CCl4 intoxication. The hepatic inflammation and fibrosis were examined with HE and Sirius red stain. The liver function including serum alanine aminotransamine (ALT)
aspartate transamine (AST)
albumin (Alb) and total bilirubin (TBIL)
liver triglyceride (TG) and malondialdehyde (MDA) contents
superoxide dismutase (SOD) activity were assayed. Hepatic hydroxyproline (Hyp) content was detected with Jamall’s method. The α-SMA expression was analyzed by immunohistochemistry and the Western blot. Liver MMP-2 mRNA was analyzed with Real-time PCR
and MMP-2/9 activities were measured with gelatin zymography and in situ zymography. Compared with the normal group
the levels of ALT
AST and TBIL
the content of Hyp
TG and MDA were remarkably increased
the Alb content and SOD activity were signifificantly decreased in the model group (P<0.05)
and higher levels of MMP-2 mRNA and MMP-2/9 activities (P<0.01)
the hepatic fatty degeneration and collagen accumulation and fifibrosis at liver were observed. Compared with the model control
DBD group showed slighter hepatic fatty degeneration and collagen deposition
and had lower levels of ALT
AST and TBIL activities
lower contents of MDA
TG and Hyp
but higher SOD level and Alb content (P<0.05)
and DBD also down-regulated MMP-2 mRNA expression and decreased MMP-2/9 activities in the fifibrotic livers (P<0.01). The action of DBD against liver fibrosis is related to prevent lipid peroxidation and inhibit MMP-2/9 activities in the fibrotic livers.
Abstract
To explore the mechanism of Danggui Buxue Decoction (当归补血汤
DBD) on the liver fifibrosis related to hepatic lipid peroxidation and matrix metalloproteinases (MMP) −2/9 activities. The liver fifibrosis in 28 rats was induced by an injection of carbon tetrachloride (CCl4) and fed with high lipid and low protein diet for 6 weeks
the model rats were randomly divided into the model group and DBD treated group
14 in each group
and another 10 rats as the normal group were observed as well. Rats in the DBD group were administered with DBD at the dose of 6 g/kg body weight for 6 weeks since CCl4 intoxication. The hepatic inflammation and fibrosis were examined with HE and Sirius red stain. The liver function including serum alanine aminotransamine (ALT)
aspartate transamine (AST)
albumin (Alb) and total bilirubin (TBIL)
liver triglyceride (TG) and malondialdehyde (MDA) contents
superoxide dismutase (SOD) activity were assayed. Hepatic hydroxyproline (Hyp) content was detected with Jamall’s method. The α-SMA expression was analyzed by immunohistochemistry and the Western blot. Liver MMP-2 mRNA was analyzed with Real-time PCR
and MMP-2/9 activities were measured with gelatin zymography and in situ zymography. Compared with the normal group
the levels of ALT
AST and TBIL
the content of Hyp
TG and MDA were remarkably increased
the Alb content and SOD activity were signifificantly decreased in the model group (P<0.05)
and higher levels of MMP-2 mRNA and MMP-2/9 activities (P<0.01)
the hepatic fatty degeneration and collagen accumulation and fifibrosis at liver were observed. Compared with the model control
DBD group showed slighter hepatic fatty degeneration and collagen deposition
and had lower levels of ALT
AST and TBIL activities
lower contents of MDA
TG and Hyp
but higher SOD level and Alb content (P<0.05)
and DBD also down-regulated MMP-2 mRNA expression and decreased MMP-2/9 activities in the fifibrotic livers (P<0.01). The action of DBD against liver fibrosis is related to prevent lipid peroxidation and inhibit MMP-2/9 activities in the fibrotic livers.
关键词
Danggui Buxue Decoctionliver fifibrosishepatic lipid peroxidationmatrix metalloproteinases-2/9
Keywords
Danggui Buxue Decoctionliver fifibrosishepatic lipid peroxidationmatrix metalloproteinases-2/9
references
Friedman SL, Rockey DC, Bissell DM. Hepatic fibrosis 2006: Report of the Third AASLD Single Topic Conference. Hepatology 2007;45:242–249.
Bataller R, Brenner DA. Liver fibrosis. J Clin Invest 2005;115:209–218.
Gao Q, Li J, Cheung JK, Duan J, Ding S, Cheung AW, et al. Verification of the formulation and efficacy of Danggui Buxue Tang (a decoction of Radix Astragali and Radix Angelicae Sinensis): an exemplifying systematic approach to revealing the complexity of Chinese herbal medicine formulae. Chin Med (Taipei) 2007;29:2–12.
Tao YY, Chen Y, Chen GF, Zhou T, Li FH, Liu CH. Antihepatic fibrosis effect of “Danggui Buxue Decoction” with different proportions of Radix Astraga and Angelica Sinensis in rats. Acta Univ Tradit Med Second Pharmacol Shanghai (Chin) 2008; 22: 40–44.
Sun BM, Xuan HP, Li FH, Hu YY, Liu P, Liu CH. Effects of Cordyceps mycelium extract on steatohepatitis and liver fibrosis in rats. Chin J New Drugs Clin (Chin) 2008; 27:6–11.
Jamall IS, Finelli VN, Que Hee SS. A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues. Anal Biochem 1981;112:70–75.
Kleiner DE, Stetler-Stevenson WG. Quantitative zymography: detection of picogram quantities of gelatinases. Anal Biochem 1994; 218:325–329.
Wielockx B, Lannoy K, Shapiro SD, Itoh T, Itohara S, Vandekerckhove J, et al. Inhibition of matrix metalloproteinases blocks lethal hepatitis and apoptosis induced by tumor necrosis factor and allows safe antitumor therapy. Nat Med 2001;7:1202–1208.
Parola M, Robino G. Oxidative stress-related molecules and liver fibrosis. J Hepatol 2001;35:297–306.
Poli G. Pathogenesis of liver fibrosis: role of oxidative stress. Mol Aspects Med 2000;21:49–98.
Galli A, Saegliati-Baroni G, Ceni E, Milani S, Ridolfi F, Salzano R, et al. Oxidative stress stimulates proliferation and invasiveness of hepatic stellate cells via a MMP2-mediated mechanism. Hepatology 2005;41:1074–1084.
Chinese Society of Liver Disenses, Chinese Association of Integrative Medicine. Guidelines for the diagnosis and treatment of liver fibrosis in integrative medicine practice. Chin J Hepatol (Chin) 2006;14:866–870.
Huang HC, Min YL, Li JZ, Wang HY. Comparative effects between Astragalus-Angelica mixture and enalapril on expression of connective tissue growth factor in rats with renal tubulointerstitial fibrosis. Chin J Neph (Chin) 2003;19: 133–136.
Milani S, Herbst H, Schuppan D, Grappone C, Pellegrini G, Pinzani M, et al. Differential expression of matrixmetalloproteinase-1 and -2 genes in normal and fibrotic human liver. Am J Pathol 1994;144:528–537.
0
浏览量
347
Downloads
14
CSCD
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 邮编：100091
- 联系电话：010-62886827, 62877592 传真：010-62874291
- 技术支持由北京北大方正电子有限公司提供 京ICP备05067934号-1
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
