Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells

Xiao-ying Wang; Xiu-mei Gao; Hong Liu; Han Zhang; Yang Liu; Min Jiang; Li-min Hu; Bo-li Zhang

doi:10.1007/s11655-010-0033-z

Your Location：

Home >

Browse articles >

Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells

OriginalPaper | Updated：2021-08-27

- Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells
- Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells
- 中国结合医学杂志（英文版） 2010年16卷第1期页码：33-40
- Affiliations：
  
  1. Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine,Tianjin,China
  2. Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin,China
  3. Key Laboratory of Traditional Chinese Medicine Pharmacology,Tianjin,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program, No. 2005CB523404), the National Natural Science Foundation of China (No. 30672631, 30572348) and the Program for New Century Excellent Talents in University (No. NCET-06-0253)
- DOI：10.1007/s11655-010-0033-z
  中图分类号：
- 纸质出版日期：2010，
  
  网络出版日期：2010-2-4，
Scan for full text
Wang, Xy., Gao, Xm., Liu, H. et al. Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells., Chin. J. Integr. Med. 16, 33–40 (2010). https://doi.org/10.1007/s11655-010-0033-z

Xiao-ying Wang, Xiu-mei Gao, Hong Liu, et al. Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells[J]. Chinese Journal of Integrative Medicine, 2010,16(1):33-40.
Wang, Xy., Gao, Xm., Liu, H. et al. Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells., Chin. J. Integr. Med. 16, 33–40 (2010). https://doi.org/10.1007/s11655-010-0033-z DOI：

Xiao-ying Wang, Xiu-mei Gao, Hong Liu, et al. Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells[J]. Chinese Journal of Integrative Medicine, 2010,16(1):33-40. DOI： 10.1007/s11655-010-0033-z.

摘要

Periplocin is an active digitalis-like component from Cortex Periplocae

which has been widely used in the treatment of heart diseases in China for many years. According to the recommendations on the cardiovascular effect of periplocin from in vivo experiments

subsequent in vitro experiments are greatly needed for the global assessment of periplocin. The objective of this study is to investigate the cell proliferation effect and the mechanism of periplocin on endothelial cells. The proliferative activity of periplocin (0.4

250 μmol/L; 6

72 h) was investigated by a comparison with the well-reported cardiac glycoside

ouabain

on mouse cardiac microvascular endothelial cells (CMEC). 3-(4

5-dimethylthiazolyl)-2

5-diphenyltetrazolium bromide (MTT)

lactate dehydrogenase (LDH) and 5-bromo-2-deoxyuridine (BrdU) assays were used to evaluate cell proliferation and viability. Subsequently

cDNA microarray experiments were performed on periplocin- (50 μmol/L) and ouabain- (50 μmol/L) treated cells

and data was analyzed by ArrayTrack software. Periplocin could increase cell viability to a level lower than ouabain in the MTT analysis

but decrease LDH release simultaneously. The BrdU incorporation assay showed an increase in cell proliferation with 2–50 μmol/L periplocin. Genes related to protein serine/threonine kinase were the most significantly enriched in the 160 genes identified in periplocin versus the control. In the 165 genes regulated by periplocin versus ouabain

GTP-binding was the most altered term. The results demonstrated the proliferation action of periplocin on CMEC. Meanwhile

its lower cytotoxicity compared to ouabain provides a new insight into the treatment of heart failure.

Abstract

Periplocin is an active digitalis-like component from Cortex Periplocae

which has been widely used in the treatment of heart diseases in China for many years. According to the recommendations on the cardiovascular effect of periplocin from in vivo experiments

250 μmol/L; 6

72 h) was investigated by a comparison with the well-reported cardiac glycoside

ouabain

on mouse cardiac microvascular endothelial cells (CMEC). 3-(4

5-dimethylthiazolyl)-2

5-diphenyltetrazolium bromide (MTT)

lactate dehydrogenase (LDH) and 5-bromo-2-deoxyuridine (BrdU) assays were used to evaluate cell proliferation and viability. Subsequently

cDNA microarray experiments were performed on periplocin- (50 μmol/L) and ouabain- (50 μmol/L) treated cells

and data was analyzed by ArrayTrack software. Periplocin could increase cell viability to a level lower than ouabain in the MTT analysis

GTP-binding was the most altered term. The results demonstrated the proliferation action of periplocin on CMEC. Meanwhile

its lower cytotoxicity compared to ouabain provides a new insight into the treatment of heart failure.

关键词

periplocincardiac microvascular endothelial cellsproliferationprotein serine/threonine kinaseGTP-binding

Keywords

periplocincardiac microvascular endothelial cellsproliferationprotein serine/threonine kinaseGTP-binding

references

China Pharmacopoeia Committee. Pharmacopeia of the People’s Republic of China (2005 ed). Part 1. Beijing: Chemical Industry Press; 2005:181.

Egawa K, Kurihara Y, Ito T, Matsumoto M, Nose K. Induction of p16INK4a transcription and of cellular senescence by clacinomycin-derivatives and cardiac glycosides. Biol Pharm Bull 2002;25:461–465.

Yang Q, Huang W, Jozwik C, Lin Y, Glasman M, Caohuy H, et al. Cardiac glycosides inhibit TNF-alpha/NF-kappaB signaling by blocking recruitment of TNF receptor-associated death domain to the TNF receptor. Proc Natl Acad Sci USA 2005;102:9631–9636.

Schoner W, Scheiner-Bobis G. Endogenous and exogenous cardiac glycosides and their mechanisms of action. Am J Cardiovasc Drugs 2007;7:173–189.

Umehara K, Sumii N, Satoh H, Miyase T, Kuroyanagi M, Ueno A. Studies on differentiation inducers. V. Steroid glycosides from periplocae radicis cortex. Chem Pharm Bull (Tokyo) 1995;43:1565–1568.

Wasserstrom JA, Aistrup GL. Digitalis: new actions for an old drug. Am J Physiol Heart Circ Physiol 2005;289: H1781–1793.

Li YH, Gao XM, Zhang BL, Xu Q, Liu H, Pan GX. Effect of the Chinese medicine Xiangjiapi on cardiac function of isolated hearts. J Liaoning Coll Tradit Chin Med (Chin) 2005;7:396–397.

Xie YS, Ren XL, Pan GX, Gao XM, Liu CX. The assessment of absorption of periplocin in situ via intestinal perfusion of rats by HPLC. Biomed Chromatogr 2008;22:196–201.

Trevisi L, Pighin I, Luciani S. Vascular endothelium as a target for endogenous ouabain: studies on the effect of ouabain on human endothelial cells. Cell Mol Biol (Noisy-le-grand) 2006;52:64–70.

Ren YP, Huang RW, Lu ZR. Ouabain at pathological concentrations might induce damage in human vascular endothelial cells. Acta Pharmacol Sin 2006;27:165–172.

Qiu J, Gao HQ, Liang Y, Yu H, Zhou RH. Comparative proteomics analysis reveals role of heat shock protein 60 in digoxin-induced toxicity in human endothelial cells. Biochim Biophys Acta 2008;1784:1857–1864.

Winnicka K, Bielawski K, Bielawska A, Surazyński A. Antiproliferative activity of derivatives of ouabain, digoxin and proscillaridin A in human MCF-7 and MDA-MB-231 breast cancer cells. Biol Pharm Bull 2008;31:1131–1140.

Nishida M, Carley WW, Gerritsen ME, Ellingsen O, Kelly RA, Smith TW. Isolation and characterization of human and rat cardiac microvascular endothelial cells. Am J Physiol 1993;264(2 Pt 2):639–652.

Moccia F, Villa A, Tanzi F. Flow-activated Na(+) and K(+) current in cardiac microvascular endothelial cells. J Mol Cell Cardiol 2000;32:1589–1593.

Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 1993;15:532–534, 536–537.

Sun H, Fang H, Chen T, Perkins R, Tong W. GOFFA: gene ontology for functional analysis-A FDA gene ontology tool for analysis of genomic and proteomic Data. BMC Bioinformatics 2006;7Suppl 2:S23.

Rochat-Steiner V, Becker K, Micheau O, Schneider P, Burns K, Tschopp J. FIST/HIPK3: a Fas/FADD-interacting serine/threonine kinase that induces FADD phosphorylation and inhibits fas-mediated Jun NH(2)-terminal kinase activation. J Exp Med 2000;192:1165–1174.

Inoue T, Hammaker D, Boyle DL, Firestein GS. Regulation of p38 MAPK by MAPK kinases 3 and 6 in fibroblast-like synoviocytes. J Immunol 2005;174:4301–4306.

Page C, Doubell AF. Mitogen-activated protein kinase (MAPK) in cardiac tissues. Mol Cell Biochem 1996;157:49–57.

Vallenius T, Mäkelä TP. Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. J Cell Sci 2002;115(Pt 10):2067–2073.

Groth A, Lukas J, Nigg EA, Silljé HH, Wernstedt C, Bartek J, et al. Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint. EMBO J 2003;22:1676–1687.

DeVries-Seimon TA, Ohm AM, Humphries MJ, Reyland ME. Induction of apoptosis is driven by nuclear retention of protein kinase C delta. J Biol Chem 2007;282:22307–22314.

Brodie C, Steinhart R, Kazimirsky G, Rubinfeld H, Hyman T, Ayres JN, et al. PKCdelta associates with and is involved in the phosphorylation of RasGRP3 in response to phorbol esters. Mol Pharmacol 2004;66:76–84.

Tong H, Rockman HA, Koch WJ, Steenbergen C, Murphy E. G protein-coupled receptor internalization signaling is required for cardioprotection in ischemic preconditioning. Circ Res 2004;94:1133–1141.

Zhu WZ, Wang SQ, Chakir K, Yang D, Zhang T, Brown JH, et al. Linkage of beta1-adrenergic stimulation to apoptotic heart cell death through protein kinase A-independent activation of Ca2+/calmodulin kinase II. J Clin Invest 2003;111:617–625.

Allen DG, Orchard CH. Myocardial contractile function during ischemia and hypoxia. Circ Res 1987;60:153–168.

Pittner J, Rhinehart K, Pallone TL. Ouabain modulation of endothelial calcium signaling in descending vasa recta. Am J Physiol Renal Physiol 2006;291:F761–769.

Ren YP, Lu ZR. Gene expression analysis of human vascular endothelial cells treated by ouabain in Pathological Concentration. South China J Cardiol (Chin) 2004;5:25–28.

Qiu J, Gao HQ, Zhou RH, Liang Y, Zhang XH, Wang XP, et al. Proteomics analysis of the proliferative effect of low-dose ouabain on human endothelial cells. Biol Pharm Bull 2007;30:247–253.

Zhang H, Qian DZ, Tan YS, Lee K, Gao P, Ren YR, et al. Digoxin and other cardiac glycosides inhibit HIF-1alpha synthesis and block tumor growth. Proc Natl Acad Sci USA 2008;105:19579–19586.

Wakabayashi Y, Inoue J, Takahashi Y, Matsuki A, Kosugi-Okano H, Shinbo T, et al. Homozygous deletions and point mutations of the Rit1/Bcl11b gene in gamma-ray induced mouse thymic lymphomas. Biochem Biophys Res Commun 2003;301:598–603.

FULL TEXT LINK

More>

浏览量

375

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells

Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells

Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells

Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice

Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro