Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats

Sheng-zhang Lin; Hong-fei Tong; Kang-jie Chen; He Jing; Xiao Yang; Shu-sen Zheng

doi:10.1007/s11655-010-0151-7

Your Location：

Home >

Browse articles >

Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats

OriginalPaper | Updated：2021-08-27

- Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats
- Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats
- 中国结合医学杂志（英文版） 2010年16卷第2期页码：151-156
- Affiliations：
  
  1. Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Zhejiang University,Hangzhou,China
  2. Department of General Surgery, Second Affiliated Hospital of Wenzhou Medical College,Wenzhou,China
  3. Key Lab of Combined Multi-organ Transplantation, Ministry of Health,Zhejiang,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30572395, No. 30973815).
- DOI：10.1007/s11655-010-0151-7
  中图分类号：
- 纸质出版日期：2010，
  
  网络出版日期：2010-5-16，
Scan for full text
Lin, Sz., Tong, Hf., Chen, Kj. et al. Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats., Chin. J. Integr. Med. 16, 151–156 (2010). https://doi.org/10.1007/s11655-010-0151-7

Sheng-zhang Lin, Hong-fei Tong, Kang-jie Chen, et al. Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats[J]. Chinese Journal of Integrative Medicine, 2010,16(2):151-156.
Lin, Sz., Tong, Hf., Chen, Kj. et al. Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats., Chin. J. Integr. Med. 16, 151–156 (2010). https://doi.org/10.1007/s11655-010-0151-7 DOI：

Sheng-zhang Lin, Hong-fei Tong, Kang-jie Chen, et al. Effect of emodin in suppressing acute rejection following liver allograft transplantation in rats[J]. Chinese Journal of Integrative Medicine, 2010,16(2):151-156. DOI： 10.1007/s11655-010-0151-7.

摘要

To investigate the mechanism of action of emodin for suppressing acute allograft rejection in a rat model of liver transplantation. Brown Norway (BW) recipient rats of orthotopic liver transplantation (OLT) were divided into three groups

Group A receiving isografting (with BW rats as donor)

Group B receiving allografting (with Lewis rats as donor)

Group C receiving allografting and emodin treatment (50 mg/kg daily). They were sacrificed on day 7 of post-transplantation

and their hepatic histology

plasma cytokine levels

and T-cell subset expression were detected. Compared with those in Group A

rats: in Group B exhibited severe allograft rejection with a rejection activity index (RAI) of 7.67±0.98

extensive hepatocellular apoptosis with an apoptosis index (AI) of 35.83±2.32

and elevated plasma levels of interleukin-2 (IL-2)

interleukin-10 (IL-10)

tumor necrosis factor-α (TNF-α)

CD4+ and CD4 CD4+/CD8+ ratio. However

recipients in Group C showed a decrease in histological grade of rejection and hepatocellular apoptosis

as well as a decrease in plasma levels of IL-2

TNF-α

CD4+ and CD4+/CD8+ ratio

but elevated levels of IL-10 as compared with the allograft group. Post-OLT acute rejection could be attenuated by emodin

its mechanism of action may be associated with protecting hepatocytes from apoptosis

polarizing the Th 1 paradigm to Th2

and inhibiting the proliferation of CD4+ T cell in plasma.

Abstract

Group A receiving isografting (with BW rats as donor)

Group B receiving allografting (with Lewis rats as donor)

Group C receiving allografting and emodin treatment (50 mg/kg daily). They were sacrificed on day 7 of post-transplantation

and their hepatic histology

plasma cytokine levels

and T-cell subset expression were detected. Compared with those in Group A

rats: in Group B exhibited severe allograft rejection with a rejection activity index (RAI) of 7.67±0.98

extensive hepatocellular apoptosis with an apoptosis index (AI) of 35.83±2.32

and elevated plasma levels of interleukin-2 (IL-2)

interleukin-10 (IL-10)

tumor necrosis factor-α (TNF-α)

CD4+ and CD4 CD4+/CD8+ ratio. However

recipients in Group C showed a decrease in histological grade of rejection and hepatocellular apoptosis

as well as a decrease in plasma levels of IL-2

TNF-α

CD4+ and CD4+/CD8+ ratio

but elevated levels of IL-10 as compared with the allograft group. Post-OLT acute rejection could be attenuated by emodin

its mechanism of action may be associated with protecting hepatocytes from apoptosis

polarizing the Th 1 paradigm to Th2

and inhibiting the proliferation of CD4+ T cell in plasma.

关键词

Emodinliver transplantationacute allograft rejectionapoptosisimmuno-suppression

Keywords

Emodinliver transplantationacute allograft rejectionapoptosisimmuno-suppression

references

Keeffe EB. Liver transplantation: current status and novel approaches to liver replacement. Gastroenterology 2001; 120:749–762.

Encke J, Uhl W, Stremmel W, Sauer P. Immunosuppression and modulation in liver transplantation. Nephrol Dial Transplant 2004;19:22–25.

Casas-Melley AT, Falkenstein KP, Flynn LM, Ziegler VL, Dunn SP. Improvement in renal function and rejection control in pediatric liver transplant recipients with the introduction of sirolimus. Pediatr Transplant 2004;8:362–326.

Kuo YC, Tsai WJ, Meng HC, Chen WP, Yang LY, Lin CY. Immune reponses in human mesangial cells regulated by emodin from Polygonum hypoleucum Ohwi. Life Sci 2001;68:1271–1286.

Kuo YC, Meng HC, Tsai WJ. Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi. Inflamm Res 2001;50:73–82.

Huang HC, Chang JH, Tung SF, Wu RT, Foegh ML, Chu SH. Immunosuppressive effect of emodin, a free radical generator. Eur J Pharmacol 1992;211:359–364.

Lin SZ, Xu XC, Tong HF, Yu YJ, You T. Inhibition of acute rejection of allogenic rat liver transplantation by emodin. Chin J Organ Transplant (Chin) 2007;28;147–149.

Kobayashi E, Kamada N, Goto S, Miyata M. Protocol for the technique of orthotopic liver transplantation in the rat. Microsurgery 1993;14:541–546.

Tsuchimoto S, Kusumoto K, Nakajima Y, Kakita A, Uchino J, Natori T, et al. Orthotopic liver transplantation in the rat. A simplified technique using the cuff method for suprahepatic vena cava anastomosis. Transplantation 1988;45:1153–1155.

Banff schema for grading liver allograft rejection: an international consensus document. Hepatology 1997;25:658–663.

Natori S, Selzner M, Valentino KL, Fritz LC, Srinivasan A, Clavien PA, et al. Apoptosis of sinusoidal endothelial cells occurs during liver preservation injury by a caspase-dependent mechanism. Transplantation 1999;68:89–96.

Sato M, Maulik G, Bagchi D, Das DK. Myocardial protection by protykin, a novel extract of trans-resveratrol and emodin. Free Radic Res 2000;32:135–144.

Lee HZ. Effects and mechanisms of emodin on cell death in human lung squamous cell carcinoma. Br J Pharmacol 2001;134:11–20.

Huang HC, Lee CR, Chao PD, Chen CC, Chu SH. Vasorelaxant effect of emodin, an anthraquinone from a Chinese herb. Eur J Pharmacol 1991;205:289–294.

Song E, Su F, Chen J, Ou Q, Wang M, Exton MS. Blocking CTL-based cytotoxic pathways reduces apoptosis of transplanted hepatocytes. J Surg Res 2001;99;61–69.

Song E, Chen J, Antus B, Su F, Wang M, Exton MS. Adenovirus-mediated Bcl-2 gene transfer inhibits apoptosis and promotes survival of allogeneic transplanted hepatocytes. Surgery 2001;130: 502–511.

Yamamoto H, Ohdan H, Shintaku S, Asahara T, Ito H, Dohi K. Role of the bcl-2/bax pathway in hepatocyte apoptosis during acute rejection after rat liver transplantation. Transplant Int 1998; 11:179–84.

Memon MA, DKarademir S, Shen J, Koukoulis G, Fabreg, F, Williams JW, et al. Seventh day syndrome-acute hepatocyte apoptosis associated with a unique syndrome of graft loss following liver transplantation. Liver 2001;21: 13–17.

Gras J, Wieers G, Vaerman JL, Truong DQ, Sokal E, Otte JB, et al. Early immunological monitoring after pediatric liver transplantation: cytokine immune deviation and graft acceptance in 40 recipients. Liver Transplant 2007;13:426–433.

Zhang AB, Zheng SS. Strong additive effect of calcitriol and cyclosporine A on lymphocyte proliferation in vitro and rat liver allotransplantations in vivo. Chin Med J 2006;119: 2090–2095.

Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 1986;136:2348–2357.

Strom TB, Roy-Chaudhury P, Manfro R, Zheng XX, Nickerson PW, Wood K, et al. The Th1/Th2 paradigm and the allograft response. Curr Opin Immunol 1996;8:688–693.

Nickerson P, Steurer W, Steiger J, Zheng X, Steele AW, Strom TB. Cytokines and the Th1/Th2 paradigm in transplantation. Curr Opin Immunol 1994;6:757–764.

Chen Y, Chen J, Liu Z, Liang S, Luan X, Long F, et al. Relationship between TH1/TH2 cytokines and immune tolerance in liver transplantation in rats. Transplantant Proc 2008;40:2691–2695.

Fung J, Kelly D, Kadry Z, Patel-Tom K, Eghtesad B. Immunosuppression in liver transplantation: beyond calcineurin inhibitors. Liver Transplant 2005;11:2267–80.

Fujimoto N, Ishida H, Nakamura I, Ogasawara K, Itoh Y. Quantities of interleukin-12p40 in mature CD8alpha negative dendritic cells correlate with strength of TCR signal and determine Th cell development. Microbiol Immunol 2003;47:1017–1024.

FULL TEXT LINK

More>

浏览量

353

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effect of emodin on biological behavior of fibroblasts in lupus nephritis

Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells

The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells

Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells