Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis

Lei Wang; Hui Zhao; Yong-ping Fan; Hai-yang Gong; Ming Li; Fang Qi; Yan Liu

doi:10.1007/s11655-010-0167-z

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Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis

OriginalPaper | Updated：2021-08-27

- Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis
- Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis
- 中国结合医学杂志（英文版） 2010年16卷第2期页码：167-172
- Affiliations：
  
  1. School of Traditional Chinese Medicine, Capital Medical University,Beijing,China
  2. Beijing Tiantan Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30873230) and Beijing Natural Science Foundation (No. 7062022, No. 7092014)
- DOI：10.1007/s11655-010-0167-z
  中图分类号：
- 纸质出版日期：2010，
  
  网络出版日期：2010-5-16，
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Wang, L., Zhao, H., Fan, Yp. et al. Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis., Chin. J. Integr. Med. 16, 167–172 (2010). https://doi.org/10.1007/s11655-010-0167-z

Lei Wang, Hui Zhao, Yong-ping Fan, et al. Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis[J]. Chinese Journal of Integrative Medicine, 2010,16(2):167-172.
Wang, L., Zhao, H., Fan, Yp. et al. Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis., Chin. J. Integr. Med. 16, 167–172 (2010). https://doi.org/10.1007/s11655-010-0167-z DOI：

Lei Wang, Hui Zhao, Yong-ping Fan, et al. Research on the mechanism of Zuogui Pill (左归丸) and Yougui Pill (右归丸) in promoting axonal regeneration in model rats of autoimmune encephalomyelitis[J]. Chinese Journal of Integrative Medicine, 2010,16(2):167-172. DOI： 10.1007/s11655-010-0167-z.

摘要

To study the molecular mechanism of Zuogui Pill (左归丸

ZGP) and Yougui Pill (右归丸

YGP) on axonal regeneration in rats with experimental autoimmune encephalomyelitis (EAE). EAE rat model was established by bilateral rear pedes subcutaneous injection of antigen made by mixing myelin basic protein (MBP) and complete Freud’s adjuvant (CFA) in the volume ratio of 1:1. The pathological changes of axonal injury and regeneration in the brain and the spinal cord were observed on the 14th (the acute stage) and the 28th day (the remission stage) after modeling

with hematoxylin-eosin (HE) staining

silver stain

and immunohistochemical staining. The rats treated with prednisone acetate were taken as controls. Observation under the light microscope with HE staining showed a sleeve-like change in rats’ cerebrospinal parenchyma with inflammatory cell infiltration around the small vessels and neuronic denaturation

while silver staining showed excessive tumefaction and abscission of axon

and immunohistochemical analysis showed decreasing of nerve growth factor (NGF) expression at the acute stage of EAE

which was even more remarkable at the remission stage

showing significant difference as compared with the normal control (P<0.05). And the expressions of Nogo A

an axon growth inhibitor

and its receptor (Nogo-66 receptor

Ng R) were significantly higher than those in the normal control at the acute stage (P<0.01). However

after the intervention of ZGP and YGP

the pathological changes and axon damage in rats’ brain and spinal cord were much more alleviated

and the NGF expression was significantly higher than that in the model group at the acute stage (P<0.05). The expression of NGF was even stronger during the remission stage

and a better effect was shown by YGP. As for Nogo A and Ng R expressions

they were significantly lower than those in the model group at the acute stage (P<0.05)

but a better effect was shown by ZGP. ZGP and YGP can prevent axonal injury and promote the axonal regeneration in rats of EAE

and the possible mechanism is to increase the expression of NGF and reduce the expression of Nogo A and its receptor. However

some differences are observed between the two Chinese preparations in their acting times and points

which provides a certain basis for revealing the modern connotation of the Chinese medicine theory on tonifying Shen (肾)-yin and Shen-yang.

Abstract