Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy

Jun-ling Liu; Shu-ping Chen; Yong-hui Gao; Fan-ying Meng; Shu-bin Wang; Jun-ying Wang

doi:10.1007/s11655-010-0503-3

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Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy

OriginalPaper | Updated：2021-08-27

- Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy
- Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy
- 中国结合医学杂志（英文版） 2010年16卷第4期页码：315-323
- Affiliations：
  
  Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30472241, No. 90709031) and the Major State Basic Research Development Program of China (973 Program, No. 2007CB512505)
- DOI：10.1007/s11655-010-0503-3
  中图分类号：
- 纸质出版日期：2010，
  
  网络出版日期：2010-8-10，
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Liu, Jl., Chen, Sp., Gao, Yh. et al. Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy., Chin. J. Integr. Med. 16, 315–323 (2010). https://doi.org/10.1007/s11655-010-0503-3

Jun-ling Liu, Shu-ping Chen, Yong-hui Gao, et al. Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy[J]. Chinese Journal of Integrative Medicine, 2010,16(4):315-323.
Liu, Jl., Chen, Sp., Gao, Yh. et al. Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy., Chin. J. Integr. Med. 16, 315–323 (2010). https://doi.org/10.1007/s11655-010-0503-3 DOI：

Jun-ling Liu, Shu-ping Chen, Yong-hui Gao, et al. Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy[J]. Chinese Journal of Integrative Medicine, 2010,16(4):315-323. DOI： 10.1007/s11655-010-0503-3.

摘要

To explore the mechanism of electroacupuncture (EA)-induced cumulative analgesic effects on chronic pain in rats with or without ovariectomy (OVX). A total of 110 female Wistar rats were randomized into normal control (n=10)

chronic constrictive injury (CCI

n=10)

CCI+EA (n=30)

OVX+CCI (n=30)

and OVX+CCI+EA (n=30) groups. Each of the latter 3 groups was further divided into 2 days (2 d)

2 weeks (2 W) and 3 weeks (3 W) subgroups

respectively (n=10 in each subgroup). The CCI pain model was established by ligature of the right sciatic nerve

and the memory impairment model duplicated by OVX. The paw withdrawal latency (PWL

pain threshold) of the bilateral footplates was detected by radiant heat irradiation

and the bilateral difference in PWL (PWLD) was used to evaluate changes in the pain reaction. Morris water maze test was conducted for evaluating the rats’ learning-memory ability. EA was applied to bilateral Zusanli (ST36) and Yanglingquan (GB34) for 2 d

2 W and 3 W

respectively. Pituitary and hypothalamic β-endorphin (EP) and adrenocorticotrophic hormone (ACTH) contents were detected by immunoradioassay. Compared with the CCI group

PWLD of the CCI+EA-3 W group decreased signifificantly (P<0.05). Compared with the OVX+CCI group

PWLD of the OVX+CCI+EA-3 W group was lowered considerably (P<0.05)

but the value was markedly higher than its basal value and those of the normal control and CCI+EA groups (P<0.05). In comparison with the sham-OVX group

the escape latency

swimming distance (SD) in the target quadrant and total SD were increased remarkably in the OVX group (P<0.05)

while the number of target platform crossings was decreased signifificantly (P<0.05)

suggesting an impairment of the OVX rats’ learning-memory ability. In simple CCI rats

both β-EP and ACTH contents of the pituitary increased markedly (P<0.05)

and those of the hypothalamus decreased obviously compared to the normal control group (P<0.05). After EA

pituitary and hypothalamic ACTH levels were signifificantly lowered at 2 d and hypothalamic ACTH and β-EP contents increased obviously at 3 W in comparison with the CCI group (P<0.05). In OVX+CCI rats

following EA

pituitary β-EP contents at 2 d

2 W and 3 W

and hypothalamic β-EP and ACTH contents at 2 W and hypothalamic ACTH levels at 3 W increased signifificantly (P<0.05)

but hypothalamic β-EP level at 3W decreased markedly (P<0.05). The effects of repeated EA in lowering pituitary ACTH and raising hypothalamic β-EP and ACTH levels disappeared after OVX+CCI. Repeated EA has a cumulative analgesic effect

which is closely associated with its effects in regulating pituitary and hypothalamicβ-EP and ACTH levels. OVX may weaken the analgesic effect of EA by affecting hypothalamic-pituitary axis activity.

Abstract

chronic constrictive injury (CCI

n=10)

CCI+EA (n=30)

OVX+CCI (n=30)

and OVX+CCI+EA (n=30) groups. Each of the latter 3 groups was further divided into 2 days (2 d)

2 weeks (2 W) and 3 weeks (3 W) subgroups

respectively (n=10 in each subgroup). The CCI pain model was established by ligature of the right sciatic nerve

and the memory impairment model duplicated by OVX. The paw withdrawal latency (PWL

pain threshold) of the bilateral footplates was detected by radiant heat irradiation

2 W and 3 W

respectively. Pituitary and hypothalamic β-endorphin (EP) and adrenocorticotrophic hormone (ACTH) contents were detected by immunoradioassay. Compared with the CCI group

PWLD of the CCI+EA-3 W group decreased signifificantly (P<0.05). Compared with the OVX+CCI group

PWLD of the OVX+CCI+EA-3 W group was lowered considerably (P<0.05)

but the value was markedly higher than its basal value and those of the normal control and CCI+EA groups (P<0.05). In comparison with the sham-OVX group

the escape latency

swimming distance (SD) in the target quadrant and total SD were increased remarkably in the OVX group (P<0.05)

while the number of target platform crossings was decreased signifificantly (P<0.05)

suggesting an impairment of the OVX rats’ learning-memory ability. In simple CCI rats

both β-EP and ACTH contents of the pituitary increased markedly (P<0.05)

and those of the hypothalamus decreased obviously compared to the normal control group (P<0.05). After EA

following EA

pituitary β-EP contents at 2 d

2 W and 3 W

and hypothalamic β-EP and ACTH contents at 2 W and hypothalamic ACTH levels at 3 W increased signifificantly (P<0.05)

关键词

chronic neuropathic painrepeated electroacupuncturecumulative effectpituitaryhypothalamusadrenocorticotropic hormoneβ-endorphin

Keywords

chronic neuropathic painrepeated electroacupuncturecumulative effectpituitaryhypothalamusadrenocorticotropic hormoneβ-endorphin

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