Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways

Xian-qian Li; Shi-he Shao; Gui-lian Fu; Xiao-hong Han; Hong Gao

doi:10.1007/s11655-010-0538-5

Your Location：

Home >

Browse articles >

Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways

OriginalPaper | Updated：2021-08-27

- Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways
- Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways
- 中国结合医学杂志（英文版） 2010年16卷第5期页码：448-452
- Affiliations：
  
  1. Pathogenic Microorganism Department, School of Medical Science and Laboratory Medicine, Jiangsu University,Jiangsu Province,Zhenjiang,China
  2. Laboratory Center, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China
  3. Department of Biochemistry and Molecular Biology, Department of Hematology and Oncology, University of Miami Miller School of Medicine,Miami,USA
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 2007Z002A), Grants of Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine and Grants from the Department of Biochemistry and Molecular Biology, Department of Hematology and Oncology, University of Miami Miller School of Medicine
- DOI：10.1007/s11655-010-0538-5
  中图分类号：
- 纸质出版日期：2010，
  
  网络出版日期：2010-9-25，
Scan for full text
Li, Xq., Shao, Sh., Fu, Gl. et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways., Chin. J. Integr. Med. 16, 448–452 (2010). https://doi.org/10.1007/s11655-010-0538-5

Xian-qian Li, Shi-he Shao, Gui-lian Fu, et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways[J]. Chinese Journal of Integrative Medicine, 2010,16(5):448-452.
Li, Xq., Shao, Sh., Fu, Gl. et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways., Chin. J. Integr. Med. 16, 448–452 (2010). https://doi.org/10.1007/s11655-010-0538-5 DOI：

Xian-qian Li, Shi-he Shao, Gui-lian Fu, et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways[J]. Chinese Journal of Integrative Medicine, 2010,16(5):448-452. DOI： 10.1007/s11655-010-0538-5.

摘要

To investigate the mechanism of norcantharidin (NCTD)-induced SMMC-7721 hepatoma cell apoptosis. SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annexin V/propidium iodide staining. The mitochondrial membrane potential was measured by flow cytometry. Western blot analysis was used to evaluate the level of cytochrome c

caspase-3

AIF

Bcl-2 and Bax expression. NCTD inhibited SMMC-7721 cell growth in a time- and dose-dependent manner. The cells treated with NCTD showed the loss of mitochondrial membrane potential. The activities of caspase-3

cytochrome c

AIF

and Bax were up-regulated after NCTD treatment at different doses. The expression of Bcl-2 was decreased after treatment with NCTD. NCTD could induce SMMC-7721 cell apoptosis. The activation of the mitochondrial pathway was involved in the process of NCTD-induced SMMC-7721 cell apoptosis.

Abstract

caspase-3

AIF

cytochrome c

AIF

关键词

norcantharidinLiver Cancerapoptosismitochondrial pathway

Keywords

norcantharidinLiver Cancerapoptosismitochondrial pathway

references

Sun ZX, Ma QW, Zhao TD, Wei YL, Wang GS, Li JS. Apoptosis induced by norcantharidin in human tumor cells. World J Gastroenterol 2000;6:263–265.

Li LK, Abdel Fattah E, Cao GW, Ren CZ, Yang G, Gotsov AA, et al. Glioma pathogenesis-related protein 1 exerts tumor suppressor activities through proapoptotic reactive oxygen species-c-Jun-NH2 kinase signaling. Cancer Res 2008;68:434–443.

An WW, Wang MW, Tashiro SI, Onodera Satoshi, Ikejima T. Mitogen-activated protein kinase-dependent apoptosis in norcan-tharidin-treated A375-S2 cells is proceeded by the activation of protein kinase C. Chin Med J 2005;118:198–203.

Aneja R, Liu M, Yates C, Gao J, Dong X, Zhou B, et al. Multidrug resistance-associated protein-overexpressing teniposide-resistant human lymphomas undergo apoptosis by a tubulin-binding agent. Cancer Res 2008;68:1495–1503.

Kim EH, Yoon MJ, Kim SU, Kwon TK, Sohn S, Choi KS. Arsenic trioxide sensitizes human glioma cells, but not normal astrocytes, to TRAIL-induced apoptosis via CCAAT/enhancer-binding protein homologous protein-dependent DR5 up-regulation. Cancer Res 2008;68:266–275.

Hung JH, Lu YS, Wang YC, Ma YH, Wang DS, Kulp SK, Muthusamy N, Byrd JC, Cheng AL, Chen CS. FTY720 induces apoptosis in hepatocellular carcinoma cells through activation of protein kinase c signaling. Cancer Res 2008;68:1204–1212.

Lu M, Xia LJ, Hua HM, Jing YK. Acetyl-keto-boswellic acid induces apoptosis through a death receptor 5-mediated pathway in prostate cancer cells. Cancer Res 2008;68:1180–1186.

Vogelstein B, Kinzler KW. Cancer genes and the pathways they control. Nat Med 2004;10:789–799.

Lu Y, Jiao R, Chen X. Methylene blue-mediated photodynamic therapy induces mitochondria-dependent apoptosis in HeLa cell. J Cell Biochem 2008;105:1451–1460.

Lartique L, Medina C, Schembri L. An intracellular wave of cytochrome c propagates and precedes Bax redistribution during apoptosis. J Cell Sci 2008;121:3515–3523.

Mandal C, Dutta A, Mallick A. Withaferin A induces apoptosis by activating p38 mitogen-activated protein kinase signaling cascade in leukemic cells of lymphoid and myeloid origin through mitochondrial death cascade. Apoptosis 2008;13:1450–1464.

Yu J, Wang P, Ming L, Wood MA, Zhang L. SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events. Oncogene 2007;26:4189–4198.

Yang JS, Chen GW, Hsia TC. Diallyl disulfide induces apoptosis in human colon cancer cell line (COLO 205) through the induction of reactive oxygen species, endoplasmic reticulum stress, caspases casade and mitochondrial-dependent pathways. Food Chem Toxicol 2009;47:171–179.

Han YH, Kim Sz, Kim SH. Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis. Chenico-biological Interactions 2009;177:107–114.

Hansen TM, Smith DJ, Nagley P. Smac/DIABLO is not released from mitochondria during apoptotic signalling in cells deficient in cytochrome c. Cell Death Differ 2006;13:1181–1190.

Lassus P, Opitz-Araya X, Lazebnik Y. Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 2002;297:1352–1354.

Lu YY, Chen TS, Qu JL, Pan WL, Sun L, Wei XB. Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells. J Biomed Sci 2009;16(16):1–15.

Li Xian, Xing D, Wang J, Zhu DB, Zhang L, Chen XJ, et al. Effects of IκBα and its mutants on NF-κB and p53 signaling pathways. World J Gastroenterol 20 2006;12:6658–6664

Sheng X, Sun Y, Yin Y. Cirsilineol inhibits proliferation of cancer cells by inducing apoptosis via mitochondrial pathway. J Pharm Pharmacology 2008;60:1523–1529.

Oltersdorf T, Elmore SW, Shoemaker AR. An inhibitor of Bcl-2 family proteins induces regression of solid tumours. Nature 2005;435:677–681.

Saradha B, Vaithinathan S, Mathur PP. Lindane induces testicular apoptosis in adult Wistar rats through the involvement of Fas-FasL and mitochondria-dependent pathways. Toxicology 2009;255:131–139.

Salido M, Gonzalez JL, Vilches J. Loss of mitochondrial membrane potential is inhibited by bombesin in etoposideinduced apoptosis in PC-3 prostate carcinoma cells. Mol Cancer Ther 2007;6:1292–1299.

Song Y, Li M, Li M, Li JC, Wei EQ. Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria. J Zhejiang Univ Sci B (Chin) 2006;7:749–756.

Lakhani SA, Masud A, Kuida K. Caspases 3 and 7: key mediators of mitochondrial events of apoptosis. Science 2006;311:847–851.

Zhao J, Jin J, Zhang X. Transfection of Smac sensitizes tumor cells to etoposide-induced apoptosis and eradicates established human hepatoma in vivo. Cancer Gene Ther 2006;13:420–427.

Dai ZJ, Wang XJ, Li ZF. Scutellaria barbate extract induces apoptosis of hepatoma H22 cells via the mitochondrial pathway involving caspase-3. World J Gastroenterol 2008;14:7321–7328.

Zhang R, Humphreys I, Sahu RP. In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis 2008;13:1465–1478.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Therapeutic Properties of Flavonoids in Treatment of Cancer through Autophagic Modulation: A Systematic Review

Flavonoid-Rich Extract of Oldenlandia diffusa (Willd.) Roxb. Inhibits Gastric Cancer by Activation of Caspase-Dependent Mitochondrial Apoptosis

Naringin Inhibits Colorectal Carcinogenesis by Inhibiting Viability of Colorectal Cancer Cells