Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)

Qing-hui Qi; Yi Li; Chen-hui Yao; Guo-gang Liang; Hui-shu Guo

doi:10.1007/s11655-010-0539-4

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Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)

OriginalPaper | Updated：2021-08-27

- Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)
- Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)
- 中国结合医学杂志（英文版） 2010年16卷第5期页码：422-429
- Affiliations：
  
  1. Department of General Surgery, First Affiliated Hospital, Dalian Medical University,Liaoning Province,Dalian,China
  2. School of Medicine, Nanjing University Nanjing,Nanjing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30772860 and No. 30800382)
- DOI：10.1007/s11655-010-0539-4
  中图分类号：
- 纸质出版日期：2010，
  
  网络出版日期：2010-9-25，
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Qi, Qh., Li, Y., Yao, Ch. et al. Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)., Chin. J. Integr. Med. 16, 422–429 (2010). https://doi.org/10.1007/s11655-010-0539-4

Qing-hui Qi, Yi Li, Chen-hui Yao, et al. Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)[J]. Chinese Journal of Integrative Medicine, 2010,16(5):422-429.
Qi, Qh., Li, Y., Yao, Ch. et al. Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)., Chin. J. Integr. Med. 16, 422–429 (2010). https://doi.org/10.1007/s11655-010-0539-4 DOI：

Qing-hui Qi, Yi Li, Chen-hui Yao, et al. Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤)[J]. Chinese Journal of Integrative Medicine, 2010,16(5):422-429. DOI： 10.1007/s11655-010-0539-4.

摘要

To observe the effects of Dachengqi Decoction (大承气汤

DCQD) on morphological changes in the network of enteric nerve-interstitial cells of Cajal (ICCs)-smooth muscle cells (SMC) of enteric deep muscular plexuses (DMP) in the rats with multiple organ dysfunction syndrome (MODS). One hundred Wistar rats of both sexes weighing 200 to 250 g were randomly divided into the control group

MODS group

and DCQD group. The morphologic changes of enteric nerve-ICC-SMC network in the DMP of intestine was observed using c-Kit and vesicular acetylcholine transporter/neuronal nitric oxide synthase immunohistochemical double-staining with whole-mount preparation technique

confocal laser scanning microscopy

and electron microscopy. Compared with the control group

the distribution and densities of cholinergic/nitrergic nerves and ICC in the DMP (ICC-DMP) of intestine in the MODS group were significantly decreased (P<0.01)

and the network of cholinergic nerve-ICC-SMC was disrupted; and the ultrastructural features of ICC-DMP

enteric nerve

and SMC were severely damaged. After treatment with DCQD

the damage in the network of enteric nerve-ICC-SMC was significantly recovered. Compared with the MODS group

the distribution and densities of cholinergic/nitrergic nerves and ICC-DMP in the DCQD group were significantly increased (P<0.01); and the ultrastructural features of ICC-DMP

enteric nerve

smooth muscle cells were significantly recovered. DCQD can improve the gastrointestinal motility in MODS. The mechanism may be related to the effect of repairing the damages in the network of enteric nerve-ICC-SMC.

Abstract

To observe the effects of Dachengqi Decoction (大承气汤

MODS group

confocal laser scanning microscopy

and electron microscopy. Compared with the control group

the distribution and densities of cholinergic/nitrergic nerves and ICC in the DMP (ICC-DMP) of intestine in the MODS group were significantly decreased (P<0.01)

and the network of cholinergic nerve-ICC-SMC was disrupted; and the ultrastructural features of ICC-DMP

enteric nerve

and SMC were severely damaged. After treatment with DCQD

the damage in the network of enteric nerve-ICC-SMC was significantly recovered. Compared with the MODS group

the distribution and densities of cholinergic/nitrergic nerves and ICC-DMP in the DCQD group were significantly increased (P<0.01); and the ultrastructural features of ICC-DMP

enteric nerve

关键词

Dachengqi decoctionMultiple Organ Dysfunction Syndromeinterstitial cells of Cajalenteric nerve smooth muscle cells

Keywords

Dachengqi decoctionMultiple Organ Dysfunction Syndromeinterstitial cells of Cajalenteric nerve smooth muscle cells

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