Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells

Hong-jin Wu; An-ruo Zou; Fang Xie; Yi-mei Du; Yu Cao; Yu-na Liu; Ji-yuan Yang; Xin-min Li

doi:10.1007/s11655-010-9997-y

Your Location：

Home >

Browse articles >

Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells

OriginalPaper | Updated：2021-08-27

- Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells
- Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells
- 中国结合医学杂志（英文版） 2010年16卷第5期页码：430-434
- Affiliations：
  
  1. Beijing Hospital of Integrated Traditional Chinese and Western Medicine,Beijing,China
  2. Ion Channelopathy Research Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
- Author bio：
- Funds：
  
  Supported by Beijing Administration Bureau of Traditional Chinese Medicine (No. JZZ-312)
- DOI：10.1007/s11655-010-9997-y
  中图分类号：
- 纸质出版日期：2010，
  
  网络出版日期：2010-6-10，
Scan for full text
Wu, Hj., Zou, Ar., Xie, F. et al. Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells., Chin. J. Integr. Med. 16, 430–434 (2010). https://doi.org/10.1007/s11655-010-9997-y

Hong-jin Wu, An-ruo Zou, Fang Xie, et al. Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells[J]. Chinese Journal of Integrative Medicine, 2010,16(5):430-434.
Wu, Hj., Zou, Ar., Xie, F. et al. Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells., Chin. J. Integr. Med. 16, 430–434 (2010). https://doi.org/10.1007/s11655-010-9997-y DOI：

Hong-jin Wu, An-ruo Zou, Fang Xie, et al. Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells[J]. Chinese Journal of Integrative Medicine, 2010,16(5):430-434. DOI： 10.1007/s11655-010-9997-y.

摘要

To observe the effect of matrine on human ether à go-go related gene (HERG) potassium channels expressed in Chinese hamster ovary (CHO) cells and investigate whether HERG channel is a new target of the pharmacological effect of matrine on arrhythmia and tumor HERG channel potassium current in CHO cell was recorded using whole-cell patch-clamp technique

and the influence of matrine on the current was explored. Matrine inhibited HERG potassium current in a dose-dependent manner

and the 50% inhibitory concentration (IC IC50) was 411±23 μmol/L. Matrine had no significant effect on the activation kinetics

and mainly blocked HERG channels in their closed state. The blocking effect of matrine on HERG channels might be one of the mechanisms against arrythmias and tumors. Unlike most other blockers exerting blocking effect at the intracellular sites by entering the cell with the opening of HERG channel

matrine blocked HERG channels at the extracellular sites.

Abstract

and the influence of matrine on the current was explored. Matrine inhibited HERG potassium current in a dose-dependent manner

and the 50% inhibitory concentration (IC IC50) was 411±23 μmol/L. Matrine had no significant effect on the activation kinetics

matrine blocked HERG channels at the extracellular sites.

关键词

Matrinepatch-clamp techniquehuman ether à go-go related genepotassium channelChinese hamster ovary cell

Keywords

Matrinepatch-clamp techniquehuman ether à go-go related genepotassium channelChinese hamster ovary cell

references

Shah RR. Drugs, QT interval prolongation and ICH E14: the need to get it right. Drug Saf 2005; 28: 115–125.

Lastraioli E, Guasti L, Crociani O, Polvani S, Hofmann G, Witchel H, et al. herg1 gene and HERG1 protein are overexpressed in colorectal cancers and regulate cell invasion of tumor cells. Cancer Res 2004;64:606–611.

Sanguinetti MC, Tristani-Firouzi M. HERG potassium channels and cardiac arrhythmia. Nature 2006;440:463–469.

Sanguinetti MC, Jiang C, Curran ME, Keating MT. A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel. Cell 1995;81:299–307.

Zhang Y, Du J, Zhang Y, Sun HL, Pan ZW, Lu YJ, et al. Effects of matrine, oxymatrine and resveratrol on HERG channel expression. Acta Pharm Sin 2007;42:139–144.

Yatani A, Wakamori M, Mikala G, Bahinski A. Block of transient outward-type cloned cardiac K+ channel currents by quinidine. Circ Res 1993;73:351–359.

Deal KK, England SK, Tamkun MM. Molecular physiology of cardiac potassium channels. Physiol Rev 1996;76:49–67.

Amos GJ, Jacobson I, Duker G, Carlsson L. Block of HERG-carried K+ currents by the new repolarization delaying agent H345/52. J Cardiovas Electrophysiol 2003;14:651–658.

Sanchez-Chapula JA, Ferer T, Navaro-Polanco RA, Sanguinetti MC. Voltage-dependent profile of human ether-a-go-go-related gene channel block is influenced by a single residue in the S6 transmembrane domain. Mol Pharmacol 2003; 63: 1051–1058.

Tu DN, Liao YH, Zou AR, Du YM, Run Q, Wang XP, et al. Electropharmacological properties of telmisartan in blocking hKv1.5 and HERG potassium channels expressed on Xenopus laevis oocytes. Acta Pharmacol Sin 2008;29:913–922.

Su Z, Chen J, Martin RL, McDermott JS, Cox BF, Gopalakrishnan M, et al. Block of hERG channel by ziprasidone: biophysical properties and molecular determinants. Biochem Pharmacol 2006; 7l: 278–286.

Milnes JT, Dempsey CE, Ridley JM, Crociani O, Arcangeli A, Hancox JC, et al. Preferential closed channel blockade of HERG potassium currents by chemically synthesised BeKm-1 scorpion toxin. FEBS Letters 2003; 547: 20–26.

Vandenberg JI, Torres AM, Campbell TJ, Kuchel PW. The HERG K+ channel: progress in understanding the molecular basis of its unusual gating kinetics. Eur Biophys J 2004;33:89–97.

Shan HL, Yang BF, Zhou YH, Wang H, Li BX. Effects of matrine on aconitine-induced electrophysiological changes in rat ventricular myocytes. J Chin Pharm Sci 2004;13:193–198.

Yang BF, Li BX, Zhou YH, Dong DL, Shan HL, Wang L. Inhibitory effects of matrine, E-4031, dofetilide and RP58866 on inward rectifier potassium current in rabbit ventricular myocytes. Chin Pharm Toxicol 2004;18:407–414.

Zhang W, Pan ZW, Feng TM, Lu YJ, Dong DL, Yang BF. Effects of matrine on the rapid component of delayed rectifier potassium current in ischemic ventricular myocytes. Chin Pharmacol Bull 2008;24:322–326.

Zhao J, Zheng JQ. HERG gene and tumor. Tumour J World 2005;4:148–151.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes

Study on biological effect of anti-cancer herbs

Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells