Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint

Yang Cheng; Hai-hui Wang; Yi-yang Hu; Gao-feng Chen; Jian Ping; Jing-hua Peng; Qin Feng

doi:10.1007/s11655-011-0603-8

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Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint

OriginalPaper | Updated：2021-08-27

- Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint
- Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint
- 中国结合医学杂志（英文版） 2011年17卷第5期页码：361-368
- Affiliations：
  
  1. Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Institutes of Shanghai Municipal Education Commission, Shanghai,E,China
  3. Key Laboratory of Liver and Kidney Diseases, Ministry of Education,Shanghai,China
- Author bio：
- Funds：
  
  Supported by Shanghai Rising-Star Program (No. 07QA14052), E-Institutes of Shanghai Municipal Education Commission (E03008);Innovative Research Team in Universities, Shanghai Municipal Education Commission;and Shanghai Leading Academic Discipline Project of Shanghai Municipal Education Commission
- DOI：10.1007/s11655-011-0603-8
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-5-25，
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Cheng, Y., Wang, Hh., Hu, Yy. et al. Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint., Chin. J. Integr. Med. 17, 361–368 (2011). https://doi.org/10.1007/s11655-011-0603-8

Yang Cheng, Hai-hui Wang, Yi-yang Hu, et al. Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint[J]. Chinese Journal of Integrative Medicine, 2011,17(5):361-368.
Cheng, Y., Wang, Hh., Hu, Yy. et al. Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint., Chin. J. Integr. Med. 17, 361–368 (2011). https://doi.org/10.1007/s11655-011-0603-8 DOI：

Yang Cheng, Hai-hui Wang, Yi-yang Hu, et al. Structural shifts of gut flora in rat acute alcoholic liver injury and Jianpi Huoxue Decoction’s (健脾活血汤) effect displayed by ERIC-PCR fingerprint[J]. Chinese Journal of Integrative Medicine, 2011,17(5):361-368. DOI： 10.1007/s11655-011-0603-8.

摘要

To study the structural shifts of gut flora in rats with acute alcoholic liver injury (AALI)

and the effect of Jianpi Huoxue Decoction (健脾活血汤

JPHXD) on the gut flora. Thirty-six Sprague-Dawley rats were randomly allocated to the control

AALI and JPHXD groups equally. The rats in the control group were given water and those in AALI and JPHXD groups were given ethanol by intragastric gavage for 5 days

while rats in the JPHXD group were administered JPHXD simultaneously. The blood and liver tissue were collected at the end of the experiment. The activities of serum alkaline aminotransferase (ALT)

aspartate aminotransferase (AST)

hepatic γ-glutamyltranspetidase (γ-GT) and hepatic triglyceride (TG) levels were determined. Plasma endotoxin level in the portal vein was measured. Pathological changes of liver tissues were determined by hematoxylin and Eosin (HE) staining and oil red O staining. The total DNA of gut flora were extracted from fecal samples by Bead-beating method and determined by ERIC-PCR fingerprint method. The similarity cluster analysis and principal component analysis were performed to analyze the ERIC-PCR fingerprint respectively. In the AALI group

the ratio of liver/body weight

activities of ALT

AST and hepatic γ-GT

amount of hepatic TG were elevated significantly compared with those in the control group (all P<0.01). JPHXD decreased the ratio

activities of ALT

AST

γ-GT and TG significantly compared with those in the AALI group (P<0.05 or P<0.01). HE and oil red O staining showed that fat deposited markedly in liver tissue

while JPHXD alleviated pathological changes markedly. Plasma LPS level in rat portal vein in the AALI group increased significantly (P<0.01)

but it was decreased significantly in the JPHXD group (P<0.01). The cluster analysis and principal component analysis of ERIC-PCR fingerprint showed that gut flora in the AALI group changed markedly

and JPHXD could recover gut flora to some extent. The structure of gut flora shifted markedly during acute alcoholic liver injury

JPHXD had prevention effect through the modification of gut flora.

Abstract

To study the structural shifts of gut flora in rats with acute alcoholic liver injury (AALI)

and the effect of Jianpi Huoxue Decoction (健脾活血汤

JPHXD) on the gut flora. Thirty-six Sprague-Dawley rats were randomly allocated to the control

AALI and JPHXD groups equally. The rats in the control group were given water and those in AALI and JPHXD groups were given ethanol by intragastric gavage for 5 days

while rats in the JPHXD group were administered JPHXD simultaneously. The blood and liver tissue were collected at the end of the experiment. The activities of serum alkaline aminotransferase (ALT)

aspartate aminotransferase (AST)

the ratio of liver/body weight

activities of ALT

AST and hepatic γ-GT

amount of hepatic TG were elevated significantly compared with those in the control group (all P<0.01). JPHXD decreased the ratio

activities of ALT

AST

γ-GT and TG significantly compared with those in the AALI group (P<0.05 or P<0.01). HE and oil red O staining showed that fat deposited markedly in liver tissue

while JPHXD alleviated pathological changes markedly. Plasma LPS level in rat portal vein in the AALI group increased significantly (P<0.01)

but it was decreased significantly in the JPHXD group (P<0.01). The cluster analysis and principal component analysis of ERIC-PCR fingerprint showed that gut flora in the AALI group changed markedly

and JPHXD could recover gut flora to some extent. The structure of gut flora shifted markedly during acute alcoholic liver injury

JPHXD had prevention effect through the modification of gut flora.

关键词

acute alcoholic liver injurygut floraJianpi Huoxue DecoctionERIC-PCRfingerprintcluster analysisprincipal component analysis

Keywords

acute alcoholic liver injurygut floraJianpi Huoxue DecoctionERIC-PCRfingerprintcluster analysisprincipal component analysis

references

Rao R. Endotoxemia and gut barrier dysfunction in alcoholic liver disease. Hepatology (Chin) 2009;50:638–644.

Wang HH, Cheng Y, Hu YY, Chen GF, Peng JH, Feng Q, et al. Prevention effect of Jianpi Huoxue Decoction on acute alcoholic liver injury in rats. Chin J Integr Tradit West Med Liver Dis (Chin) 2009;19:221–223.

Lu HF, Wei GF, Li ZK, Li H, Wang AS, Gu JP, et al. ERICPCR based fingerprinting and molecular hybridization to analyze the characteristics of intestinal microflora of giant panda. Chin J Microecol (Chin) 2005;17: 81–84.

Xu LJ, Zhao QL, Pang XY, Zhang MH, Ni Y, Xu ZH, et al. Structural shifts of gut flora in diabetic mice displayed by ERIC-PCR fingerprinting technique. Chin J Microecol (Chin) 2007;19:324–326.

Zhao JB, Wang TL, Zhang J, Zhang BJ, Cheng XF. Rat model of acute alcoholic liver injury. J China-Japan Friendship Hosp (Chin) 1996;10:17–19.

Cheng Y, Ping J, Xu L. Effects of curcumin on peroxisome proliferator-activated receptor gamma expression and nuclear translocation/redistribution in culture-activated rat hepatic stellate cells. Chin Med J 2007;120: 794–801.

Cheng Y, Ping J, Liu C, Tan YZ, Chen GF. Study on effects of extracts from Salvia Miltiorrhiza and Curcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells. Chin J Integr Med 2006;12:207–211.

Zhang ML, Zhou ZH, Zhao LP. Polymorphism of Escherichia coli isolated from the fecal. Microbiology (Chin) 2005; 32: 5–9.

Purohit V, Bode JC, Bode C, Brenner DA, Choudhry MA, Hamilton F, et al. Alcohol, intestinal growth, intestinal permeability to endotoxin, and medical consequences: summary of a symposium. Alcohol 2008;42:349–361.

Sozinov AS, Abdulkhakov SR, Kiyasov AP, Gumerova AA. Alteration of the liver in rats with experimental dysbiosis. Bull Exp Biol Med 2003; 136: 19–21.

Streit WR, Schmitz RA. Metagenomics-the key to the uncultured microbes. Curr Opin Microbiol 2004;7:492–498.

Zhao Y, Li W, Zhou Z, Wang L, Pan Y, Zhao L. Dynamics of microbial community structure and cellulolytic activity in agricultural soil amended with two biofertilizers. Eur J Soil Biol 2005; 41:21–29.

Wei G, Pan L, Du H, Chen J, Zhao L. ERIC-PCR fingerprinting-based community DNA hybridization to pinpoint genome-specific fragments as molecular markers to identify and track populations common to healthy human guts. J Microbio Methods 2004;59:91–108.

Adamus-Bialek W, Wojtasik A, Majchrzak M, Sosnowski M, Parniewski P. (CGG)4-based PCR as a novel tool for discrimination of uropathogenic Escherichia coli strains: comparison with enterobacterial repetitive intergenic consensus-PCR. J Clin Microbiol 2009;47:3937–3944.

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