Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome

Mei Xue; Hui-jun Yin; Cai-feng Wu; Xiao-juan Ma; Chun-yu Guo; Ye Huang; Da-zhuo Shi; Ke-ji Chen

doi:10.1007/s11655-011-0604-7

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Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome

OriginalPaper | Updated：2021-08-27

- Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome
- Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome
- 中国结合医学杂志（英文版） 2013年19卷第1期页码：42-47
- Affiliations：
  
  Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by National Basic Research Program of China (No. 2006CB504803 and No. 81030063)
- DOI：10.1007/s11655-011-0604-7
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2011-12-8，
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Xue, M., Yin, Hj., Wu, Cf. et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome., Chin. J. Integr. Med. 19, 42–47 (2013). https://doi.org/10.1007/s11655-011-0604-7

Mei Xue, Hui-jun Yin, Cai-feng Wu, et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome[J]. Chinese Journal of Integrative Medicine, 2013,19(1):42-47.
Xue, M., Yin, Hj., Wu, Cf. et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome., Chin. J. Integr. Med. 19, 42–47 (2013). https://doi.org/10.1007/s11655-011-0604-7 DOI：

Mei Xue, Hui-jun Yin, Cai-feng Wu, et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome[J]. Chinese Journal of Integrative Medicine, 2013,19(1):42-47. DOI： 10.1007/s11655-011-0604-7.

摘要

To observe the regulatory effect of Chinese drugs for activating blood circulation (ABC) and for activating blood circulation and detoxifying (ABCD) on indices of thrombosis

inflammatory reaction

and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. Fifty-four rabbits were randomized into the normal control group

model group

simvastatin group (simvastatin

0.93 mg/kg per day)

ABC group [Xiongshao Capsule (芎芍胶囊)

0.07 g/kg per day]

and ABCD group [Xiongshao Capsule

0.07 g/kg per day

and Huanglian Capsule (黄连胶囊)

0.14 g/kg per day]. All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks. At the end of week 4 of giving high-fat diets

a dose of endoxitin was given by ear vein injection

and a randomized 2-week treatment was initiated. At the end of treatment

blood lipids

circulating endothelial cells

and the pathological changes of the aortic arch were assessed. The serum levels of matrix metalloproteinases (MMP-9)

tissue inhibitors to metalloproteinase (TIMP-1)

granule membrane protein-140 (GMP-140)

plasminogen activator inhibitor-1 (PAI-1)

high-sensitivity C-reactive protein (hs-CRP)

interleukin-6 (IL-6)

and tumor necrosis factor-α(TNF-α) were determined. Compared with the model group

ABCD group showed decreased serum triglyceride (TG) level

improvement in the pathological change in the aortic arch

and reduction in the number of circulating endothelial cells (4.00±1.41 per 0.9 μL for ABCD group vs 7.83±1.72 per 0.9 μL for the model group). In addition

the levels of serum GMP-140

PAI-1

and IL-6 in ABCD group were also significantly reduced [0.79±0.20 ng/mL

5.23±1.39 ng/mL

40.64±10.11 pg/mL for ABCD group vs 1.08±0.31 ng/mL

7.28±2.01 ng/mL

54.44±13.56 pg/mL for the model group

respectively

P<0.05]. A trend showing improvement in the indices of thrombosis

inflammatory reaction

and tissue damage was observed in the ABC group when compared to the model group

but the changes were not statistically significant (P>0.05). Chinese drugs for activating blood circulation and detoxifying have beneficial effects on regulating indices of thrombosis (GMP-140 and PAI-1) and inflammatory reaction (IL-6) in rabbit model with toxic-heat and blood stasis. The effect of the activating blood circulation and detoxifying drugs in regulating the levels of serum GMP-140

PAI-1

and IL-6 was superior to that of the activating blood circulation drugs.

Abstract

To observe the regulatory effect of Chinese drugs for activating blood circulation (ABC) and for activating blood circulation and detoxifying (ABCD) on indices of thrombosis

inflammatory reaction

and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. Fifty-four rabbits were randomized into the normal control group

model group

simvastatin group (simvastatin

0.93 mg/kg per day)

ABC group [Xiongshao Capsule (芎芍胶囊)

0.07 g/kg per day]

and ABCD group [Xiongshao Capsule

0.07 g/kg per day

and Huanglian Capsule (黄连胶囊)

0.14 g/kg per day]. All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks. At the end of week 4 of giving high-fat diets

a dose of endoxitin was given by ear vein injection

and a randomized 2-week treatment was initiated. At the end of treatment

blood lipids

circulating endothelial cells

and the pathological changes of the aortic arch were assessed. The serum levels of matrix metalloproteinases (MMP-9)

tissue inhibitors to metalloproteinase (TIMP-1)

granule membrane protein-140 (GMP-140)

plasminogen activator inhibitor-1 (PAI-1)

high-sensitivity C-reactive protein (hs-CRP)

interleukin-6 (IL-6)

and tumor necrosis factor-α(TNF-α) were determined. Compared with the model group

ABCD group showed decreased serum triglyceride (TG) level

improvement in the pathological change in the aortic arch

and reduction in the number of circulating endothelial cells (4.00±1.41 per 0.9 μL for ABCD group vs 7.83±1.72 per 0.9 μL for the model group). In addition

the levels of serum GMP-140

PAI-1

and IL-6 in ABCD group were also significantly reduced [0.79±0.20 ng/mL

5.23±1.39 ng/mL

40.64±10.11 pg/mL for ABCD group vs 1.08±0.31 ng/mL

7.28±2.01 ng/mL

54.44±13.56 pg/mL for the model group

respectively

P<0.05]. A trend showing improvement in the indices of thrombosis

inflammatory reaction

and tissue damage was observed in the ABC group when compared to the model group

PAI-1

and IL-6 was superior to that of the activating blood circulation drugs.

关键词

activating blood circulationactivating blood circulation and detoxifyingthrombosistissue damageinflammatory reaction

Keywords

activating blood circulationactivating blood circulation and detoxifyingthrombosistissue damageinflammatory reaction

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