In vivo and in vitro antiviral effects of berberine on influenza virus

Ying Wu; Ji-qian Li; Ye-ji Kim; Jun Wu; Qian Wang; Yu Hao

doi:10.1007/s11655-011-0640-3

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In vivo and in vitro antiviral effects of berberine on influenza virus

OriginalPaper | Updated：2021-08-27

- In vivo and in vitro antiviral effects of berberine on influenza virus
- In vivo and in vitro antiviral effects of berberine on influenza virus
- 中国结合医学杂志（英文版） 2011年17卷第6期页码：444-452
- Affiliations：
  
  Key Laboratory of Chinese Medicine on Viral Diseases, Ministry of Education, Microbiology and Immunology Department, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30772871)
- DOI：10.1007/s11655-011-0640-3
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-6-10，
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Wu, Y., Li, Jq., Kim, Yj. et al. In vivo and in vitro antiviral effects of berberine on influenza virus., Chin. J. Integr. Med. 17, 444–452 (2011). https://doi.org/10.1007/s11655-011-0640-3

Ying Wu, Ji-qian Li, Ye-ji Kim, et al. In vivo and in vitro antiviral effects of berberine on influenza virus[J]. Chinese Journal of Integrative Medicine, 2011,17(6):444-452.
Wu, Y., Li, Jq., Kim, Yj. et al. In vivo and in vitro antiviral effects of berberine on influenza virus., Chin. J. Integr. Med. 17, 444–452 (2011). https://doi.org/10.1007/s11655-011-0640-3 DOI：

Ying Wu, Ji-qian Li, Ye-ji Kim, et al. In vivo and in vitro antiviral effects of berberine on influenza virus[J]. Chinese Journal of Integrative Medicine, 2011,17(6):444-452. DOI： 10.1007/s11655-011-0640-3.

摘要

To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo. In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells. In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice. The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality. On days 2

and 6

the viral titers in the lungs were determined by hemagglutination assay; hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha (TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay; the concentrations of nitric oxide (NO) and inducible nitric oxide synthetase (iNOS) were detected by colorimetric method; reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-α and MCP-1. Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus

with the therapeutic index 9.69. In vivo

berberine decreased mice mortality from 90% to 55%

reduced virus titers in the lungs on day 2 postinfection (P<0.05). The lung histology scores were 1.50±0.67

4.50±1.00

and 5.50±1.00 in the berberine group on days 2

and 6

respectively

which were significantly reduced compared to 2.17±0.22

6.83±0.44

and 8.50±0.33 in the infected group (P<0.05). The productions of NO and iNOS were repressed by berberine compared with those in the infected group (P<0.01). The transcription and expression of TNF-α were inhibited by berberine on day 4 (P<0.01) and day 6 (P<0.05)

and those of MCP-1 were inhibited on day 6 (P<0.01) compared with the infected group. Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo. The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection

as well as improving the pathogenic changes by repressing inflammatory substances release.

Abstract

and 6

with the therapeutic index 9.69. In vivo

berberine decreased mice mortality from 90% to 55%

reduced virus titers in the lungs on day 2 postinfection (P<0.05). The lung histology scores were 1.50±0.67

4.50±1.00

and 5.50±1.00 in the berberine group on days 2

and 6

respectively

which were significantly reduced compared to 2.17±0.22

6.83±0.44

as well as improving the pathogenic changes by repressing inflammatory substances release.

关键词

in vivoin vitroantiviral effectsBerberineinfluenza virus

Keywords

in vivoin vitroantiviral effectsBerberineinfluenza virus

references

Kim HM, Lee YW, Lee KJ, Kim HS, Cho SW, van Rooijen N, et al. Alveolar macrophages are indispensable for controlling influenza viruses in lungs of pigs. J Virol 2008; 82:4265–4274.

Ouyang Q, Cicek G, Westendorp RG, Cools HJ, van der Klis RJ, Remarque EJ. Reduced IFN-gamma production in elderly people following in vitro stimulation with influenza vaccine and endotoxin. Mech Ageing Dev 2000; 121:131–137.

Englund JA, Champlin RE, Wyde PR, Kantarjian H, Atmar RL, Tarrand J, et al. Common emergence of amantadine- and rimantadine-resistant influenza A viruses in symptomatic immunocompromised adults. Clin Infect Dis 1998; 26:1418–1424.

Jefferson T, Deeks JJ, Demicheli V, Rivetti D, Rudin M. Amantadine and rimantadine for preventing and treating influenza A in adults. Cochrane Database Syst Rev 2004; (3):CD001169. [Online.]

Long JK, Mossad SB, Goldman MP. Antiviral agents for treating influenza. Cleve Clin J Med 2000;67:92–95.

Nagai T, Suzuki Y, Tomimori T, Yamada H. Antiviral activity of plant flavonoid, 5,7,4′-trihydroxy-8-methoxyflavone, from the roots of Scutellaria baicalensis against influenza A (H3N2) and B viruses. Biol Pharm Bull 1995; 18:295–299.

Kobayashi M, Davis SM, Utsunomiya T, Pollard RB, Suzuki F. Antiviral effect of gingyo-san, a traditional Chinese herbal medicine, on influenza A2 virus infection in mice. Am J Chin Med 1999; 27:53–62.

Luo Y, Hao Y, Shi TP, Deng WW, Li N. Berberine inhibits cyclin D1 expression via suppressed binding of AP-1 transcription factors to CCND1 AP-1 motif. Acta Pharmacol Sin 2008; 29:628–633.

Wu JB, Li XY, Lin L, Cui PG, Zheng JR. In vitro antiviral activity of a berberine derivant HB-13 against herpes simplex virus. Chin J Dermatol (Chin) 2007; 40:671–673.

Wu JB, Li XY, Lin L, Cui PG, Zheng JR. Research of antiviral mechanisms of action of berberine derivate HB-13 against herpes simplex virus type 2. Chin J Antibiot (Chin) 2009; 34:376–379.

Ehrlich J, Sloan BJ, Miller FA, Machamer HE. Searching for antiviral materials from microbial fermentations. Ann NY Acad Sci 1965;130:5–16.

Reed LJ, H Muench. A simple method for estimating fifty percent endpoints. Am J Hyg 1938;27:493–497.

Guo YJ, Cheng XW. Influenza virus and experimental technique. Beijing: Three Gorges Press of China (Chin) 1997:111–112.

Cooke KR, Kobzik L, Martin TR, Brewer J, Delmonte J Jr, Crawford JM, et al. An experimental model of idiopathic pneumonia syndrome after bone marrow transplantation: I. The roles of minor H antigens and endotoxin. Blood 1996; 88:3230–3239.

Sidwell RW, Huffman JH, Bailey KW, Wong MH, Nimrod A, Panet A. Inhibitory effects of recombinant manganese superoxide dismutase on influenza virus infections in mice. Antimicrob Agents Chemother 1996; 40:2626–2631.

Pereira CV, Machado NG, Oliveira PJ. Mechanisms of berberine (natural yellow 18)-induced mitochondrial dysfunction: interaction with the adenine nucleotide translocator. Toxicol Sci 2008; 105:408–417.

Wang J, Oberley-Deegan R, Wang S, Nikrad M, Funk CJ, Hartshorn KL, et al. Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-lambda 1) in response to influenza A infection. J Immunol 2009; 182:1296–1304.

Rollins BJ. Monocyte chemoattractant protein 1: a potential regulator of monocyte recruitment in inflammatory disease. Mol Med Today 1996;2:198–204.

Hussell T, Pennycook A, Openshaw PJ. Inhibition of tumor necrosis factor reduces the severity of virus-specific lung immunopathology. Eur J Immunol 2001;31:2566–2573.

Lee JS, Park SY, Thapa D, Kim AR, Shin HM, Kim JA. HMC05, herbal formula, inhibits TNF-{alpha}-induced inflammatory response in human umbilical vein endothelial cells. Evid Based Complement Alternat Med 2009 Sep 7. [Epub ahead of print]:1–11.

Stuehr DJ, Kwon NS, Nathan CF. FAD and GSH participate in macrophage synthesis of nitric oxide. Biochem Biophys Res Commun 1990;168:558–565.

Akaike T, Noguchi Y, Ijiri S, Setoguchi K, Suga M, Zheng YM, et al. Pathogenesis of influenza virus-induced pneumonia: involvement of both nitric oxide and oxygenradicals. Proc Natl Acad Sci USA 1996;93:2448–2453.

Lin KL, Suzuki Y, Nakano H, Ramsburg E, Gunn MD. CCR2+ monocyte-derived dendritic cells and exudate macrophages produce influenza-induced pulmonary immune pathology and mortality. J Immunol 2008;180:2562–2572.

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