Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease

Qin-he Yang; Si-ping Hu; Yu-pei Zhang; Huan-huan Ping; Huan-wen Yang; Tong-yan Chen; Hai-tao Liu

doi:10.1007/s11655-011-0691-5

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Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease

OriginalPaper | Updated：2021-08-27

- Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease
- Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease
- 中国结合医学杂志（英文版） 2012年18卷第10期页码：769-774
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Medical College, Jinan University,Guangzhou,China
  2. Qingyuan Maternity and Child Health Care Hospital, Qingyuan City,Guangdong Province,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30371726)
- DOI：10.1007/s11655-011-0691-5
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-9-11，
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Yang, Qh., Hu, Sp., Zhang, Yp. et al. Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease., Chin. J. Integr. Med. 18, 769–774 (2012). https://doi.org/10.1007/s11655-011-0691-5

Qin-he Yang, Si-ping Hu, Yu-pei Zhang, et al. Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease[J]. Chinese Journal of Integrative Medicine, 2012,18(10):769-774.
Yang, Qh., Hu, Sp., Zhang, Yp. et al. Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease., Chin. J. Integr. Med. 18, 769–774 (2012). https://doi.org/10.1007/s11655-011-0691-5 DOI：

Qin-he Yang, Si-ping Hu, Yu-pei Zhang, et al. Effects of different therapeutic methods and typical recipes of Chinese medicine on activation of c-Jun N-terminal Kinase in Kupffer cells of rats with fatty liver disease[J]. Chinese Journal of Integrative Medicine, 2012,18(10):769-774. DOI： 10.1007/s11655-011-0691-5.

摘要

To observe the effects of different therapeutic methods and the recipes of Chinese medicine (CM) on the activation of c-Jun N-terminal kinase (JNK) in Kupffer cells of rats with fatty liver disease and to explore the mechanisms of these therapeutic methods. By using a random number table

98 rats were randomly divided into 7 groups: control group

model group

and 5 treatment groups

including soothing Liver (Gan) recipe group

invigorating Spleen (Pi) recipe group

dispelling dampness recipe group

promoting blood recipe group

and complex recipe group. Rats in the control group were fed with normal food and distilled water by gastric perfusion

while rats in the model group were fed with high-fat food and distilled spirits by gastric perfusion. Rats in the 5 treatment groups were fed with high-fat food and corresponding recipes by gastric perfusion. Twelve weeks later

all rats were sacrificed and liver tissues were stained for pathohistological observation. Kupffer cells were isolated from livers of rats to evaluate JNK and phospho-JNK expressions by Western blotting. The grade of hepatic steatosis was higher in the model group than the control group (P<0.05). Compared with the model group

the grade of fatty degeneration in soothing Liver recipe group and invigorating Spleen recipe group were significantly ameliorated (P<0.05). Expressions of JNK and phospho-JNK in Kupffer cells were significantly higher in the model group than those in the control group (P<0.05

P<0.01). Compared with the model group

expressions of JNK in all treatment groups decreased

especially in invigorating Spleen recipe group and promoting blood recipe group (P<0.05). Compared with the model group

expressions of phospho-JNK in all treatment groups declined significantly (P<0.01)

especially in soothing Live recipe group and invigorating Spleen recipe group. The high expressions of JNK and phospho-JNK in Kupffer cells might play an important role in the pathogenesis of fatty liver disease in rats. The recipes of CM

especially invigorating Spleen recipe and soothing Liver recipe

might protect liver against injury by reducing the total JNK protein content and inhibiting the activation of JNK protein in Kupffer cells of fatty liver model rats

which showed beneficial effects on fatty liver disease.

Abstract

98 rats were randomly divided into 7 groups: control group

model group

and 5 treatment groups

including soothing Liver (Gan) recipe group

invigorating Spleen (Pi) recipe group

dispelling dampness recipe group

promoting blood recipe group

and complex recipe group. Rats in the control group were fed with normal food and distilled water by gastric perfusion

P<0.01). Compared with the model group

expressions of JNK in all treatment groups decreased

especially in invigorating Spleen recipe group and promoting blood recipe group (P<0.05). Compared with the model group

expressions of phospho-JNK in all treatment groups declined significantly (P<0.01)

especially invigorating Spleen recipe and soothing Liver recipe

might protect liver against injury by reducing the total JNK protein content and inhibiting the activation of JNK protein in Kupffer cells of fatty liver model rats

which showed beneficial effects on fatty liver disease.

关键词

fatty liver diseaseChinese MedicineKupffer cellsc-Jun N-terminal kinase

Keywords

fatty liver diseaseChinese MedicineKupffer cellsc-Jun N-terminal kinase

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