Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice

Xi-li Wu; Wang-gang Zhang; Xing-min Shi; Peng An; Wan-sen Sun; Cheng-lin Qiao; Zhu Wang

doi:10.1007/s11655-011-0693-3

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Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice

OriginalPaper | Updated：2021-08-27

- Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice
- Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice
- 中国结合医学杂志（英文版） 2011年17卷第4期页码：277-282
- Affiliations：
  
  1. Department of Integrated Chinese Traditional and Western Medicine, Shaanxi Province Key Traditional Chinese Medicine,Shaanxi,China
  2. Department of Nephrology, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University,Xi’an,China
  3. Department of Hematology, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University,Xi’an,China
  4. Medical School of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the Sci-tech Project of Shaanxi Province [No. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg 2011 Supported by the Sci-tech Project of Shaanxi Province [No. 2006K14-G2 (5)]
- DOI：10.1007/s11655-011-0693-3
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-4-21，
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Wu, Xl., Zhang, Wg., Shi, Xm. et al. Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice., Chin. J. Integr. Med. 17, 277–282 (2011). https://doi.org/10.1007/s11655-011-0693-3

Xi-li Wu, Wang-gang Zhang, Xing-min Shi, et al. Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice[J]. Chinese Journal of Integrative Medicine, 2011,17(4):277-282.
Wu, Xl., Zhang, Wg., Shi, Xm. et al. Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice., Chin. J. Integr. Med. 17, 277–282 (2011). https://doi.org/10.1007/s11655-011-0693-3 DOI：

Xi-li Wu, Wang-gang Zhang, Xing-min Shi, et al. Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice[J]. Chinese Journal of Integrative Medicine, 2011,17(4):277-282. DOI： 10.1007/s11655-011-0693-3.

摘要

To investigate the therapeutic effects and mechanisms of using artemisinin (Art) combined with glucocorticoid (GC) to treat lupus nephritis (LN) mice. (mice. to treat lupus nephritis Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table: control group

model group

prednisone group administrated with 6.45 mg/(kg·d) prednisone suspension

and Art+prednisone group administrated with 150 mg/(kg·d) Art suspension and 3.225 mg/(kg·d) prednisone suspension. A mice model of LN was established by injection with living lymph cell suspension. The changes of urine protein/24h

the expressions of GC receptor α (GRα) mRNA

GC receptor β (GRβ) mRNA in peripheral blood mononuclear cells (PBMCs)

and transcriptional coactivator P300/CBP protein in renal tissue were measured. Compared with the model group

the treatment groups had significant decrease in urine protein/24 h

and renal pathological lesion (P<0.01). In the same groups

the expression of transcriptional coactivator P300/CBP protein in renal tissue and GRα mRNA were significantly increased

and GRβ mRNA expression was significantly decreased (P<0.01). And the Art+prednisone group has a better therapeutic effect than the prednisone group (P<0.01). Art has therapeutic sensitization effects on GC in the LN mice. The underlying mechanism could be correlated with the effect of Art on the increase of the expressions of GRα mRNA and transcriptional coactivator P300 300/CBP protein in renal tissue and on the decrease of the expression of GRβ mRNA in PBMC

Abstract

model group

prednisone group administrated with 6.45 mg/(kg·d) prednisone suspension

the expressions of GC receptor α (GRα) mRNA

GC receptor β (GRβ) mRNA in peripheral blood mononuclear cells (PBMCs)

and transcriptional coactivator P300/CBP protein in renal tissue were measured. Compared with the model group

the treatment groups had significant decrease in urine protein/24 h

and renal pathological lesion (P<0.01). In the same groups

the expression of transcriptional coactivator P300/CBP protein in renal tissue and GRα mRNA were significantly increased

关键词

Artemisininglucocorticoidlupus nephritis micedrug resistance gene

Keywords

Artemisininglucocorticoidlupus nephritis micedrug resistance gene

references

Singh S, Saxena R. Lupus nephritis. Am J Med Sci 2009;337:451–460.

Bertsias G, Gordon C, Boumpas DT. Clinical trials in systemic lupus erythematosus (SLE): lessons from the past as we proceed to the future-the EULAR recommendations for the management of SLE and the use of end-points in clinical trials. Lupus 2008;17:437–442.

Houssiau FA, Ginzler EM. Current treatment of lupus nephritis. Lupus 2008;17:426–430.

Cao HW, Guo YB, Wei LZ, Wang N, Qu H, Zhou H, et al. Effect of artemisinin on lung injury in septic rats. Acta Acad Med Milit Tertiae (Chin) 2007;29:951–954.

Aldieri E, Atragene D, Bergandi L, Riganti C, Costamagna C, Bosia A, et al. Artemisinin inhibits inducible nitric oxide synthase and nuclear factor NF-B activation. FEBS Lett 2003;552:141–144.

Sadava D, Phillips T, Lin C, Kane SE. Transferrin overcomes drug resistance to artemisinin in human small-cell lung carcinoma cells. Cancer Lett 2002;179:151–156.

Singh NP, Lai HC. Artemisinin induces apoptosis in human cancer cells. Anticancer Res 2004;24:2277–2280.

Xie H, Yao L, Chen LJ, Hu WL. Effect of artemisinin on caspase-3 and cytochrome c of leukemia cell k562. Chin Arch Tradit Chin Med (Chin) 2008;26:2648–2650.

Guo Y, Wang J, Zhang BC, Chen ZT, Zhao Y, Gao JF. Effect of artemisinin on cell apoptosis and lymph angiogenesis in mouse lymphatic endothelial cells. Med J Wuhan Univ (Chin) 2007;28:192–196.

Tanaka H, Akama H, Ichikawa Y, Makino I, Homma M. Glucocorticoid receptor in patients with lupus nephritis: relationship between receptor levels in mononuclear leukocytes and effect of glucocorticoid therapy. J Rheumatol 1992;19:878–883.

Goodman RH, Smolik S. CBP/P300 in cell growth, transformation, and development. Genes Dev 2000;14:1553–1577.

Bruijn JA, van Elven EH, Hogendoorn PC, Corver WE, Hoedemaeker PJ, Fleuren GJ. Murine chronic graft-versus-host disease as a model for lupus nephritis. Am J Pathol 1988;130:639–642.

Rabbani M, Habib H, Islam M, Ahmad B, Majid S, Saeed W, et al. Survival analysis and prognostic indicators of systemic lupus erythematosus in Pakistani patients. Lupus 2009;18:848–855.

Lu CS, Ye RG, Sun L, Yang NS, Li HQ, Liu GX. Survival probability and predictors of death in patients with lupus nephritis. Chin J Intern Med (Chin) 1998;37:250–253.

Li JH, Liu JF, Zhai N, Song FJ. Expression of glucocorticoid receptor-alpha and beta mRNA in peripheral blood mononuclear cells in patients with lupus nephritis. Chin J Lepr Skin Dis (Chin) 2005;21:166–168.

Leung DY, Hamid Q, Vottero A, Szefler SJ, Surs W, Minshall E, et al. Association of glucocorticoid insensitivity with increased expression of glucocorticoid receptor beta. J Exp Med 1997;186:1567–1574.

Liu YJ, Song LN, Li BC, Lu J. The expression of glucocorticoid receptor β messenger RNA in peripheral white blood cells of hormone-resistant nephritic syndrome patients. Chin J Intern Med (Chin) 2001;40:725–728.

Tran NQ, Crowe DL. Regulation of the human involucrin gene promoter by co-activator proteins. Biochem J 2004;381:267–273.

Glass CK, Rosenfeld MG. The coregulator exchange in transcriptional functions of nuclear receptors. Genes Dev 2000;14:121–141.

Kobayashi Y, Kitamoto T, Masuhiro Y, Watanabe M, Kase T, Metzger D, et al. p300 mediates functional synergism between AF-1 and AF-2 of estrogen receptor alpha and beta by interacting directly with the N-terminal A/B domains. J Biol Chem 2000;275:15645–15651.

Kino T, Gragerov A, Slobodskaya O, Tsopanomichalou M, Chrousos GP, Pavlakis GN. Human immunodeficiency virus type 1 (HIV-1) accessory protein Vpr induces transcription of the HIV-1 and glucocorticoid-responsive promoters by binding directly to P300/CBP coactivators. J Virol 2002;76:9724–9734.

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