Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway

Na Liu; Jun-tian Liu; Yuan-yuan Ji; Pei-pei Lu

doi:10.1007/s11655-011-0696-0

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Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway

OriginalPaper | Updated：2021-08-27

- Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway
- Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway
- 中国结合医学杂志（英文版） 2012年18卷第5期页码：371-377
- Affiliations：
  
  1. Department of Pharmacology, Xi’an Jiaotong University School of Medicine,Xi’an,China
  2. Department of Pharmacy, the Second Affiliated Hospital, Xi’an Jiaotong University School of Medicine,Xi’an,China
  3. Scientific Research and Experiment Center, the Second Affiliated Hospital, Xi’an Jiaotong University School of Medicine,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30572347)
- DOI：10.1007/s11655-011-0696-0
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2011-4-26，
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Liu, N., Liu, Jt., Ji, Yy. et al. Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway., Chin. J. Integr. Med. 18, 371–377 (2012). https://doi.org/10.1007/s11655-011-0696-0

Na Liu, Jun-tian Liu, Yuan-yuan Ji, et al. Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway[J]. Chinese Journal of Integrative Medicine, 2012,18(5):371-377.
Liu, N., Liu, Jt., Ji, Yy. et al. Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway., Chin. J. Integr. Med. 18, 371–377 (2012). https://doi.org/10.1007/s11655-011-0696-0 DOI：

Na Liu, Jun-tian Liu, Yuan-yuan Ji, et al. Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway[J]. Chinese Journal of Integrative Medicine, 2012,18(5):371-377. DOI： 10.1007/s11655-011-0696-0.

摘要

To investigate effects of Dahuang Zhechong Pill (大黄{ie371-1}虫丸

DHZCP) on the cell cycle and the related signal pathways in vascular smooth muscle cells (VSMCs) stimulated by platelet-derived growth factor (PDGF) with the method of serum pharmacology. DNA synthesis in VSMCs was examined by detecting 5′-bromo-2′-deoxyuridine incorporation with the immunocytochemical method. The cycle of VSMCs was evaluated with flow cytometry. Expressions of cyclin D1

p27

protein kinase Cα (PKCα)

and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) were quantified by Western blot method. DHZCP containing serum significantly inhibited DNA synthesis of PDGF-stimulated VSMCs

arrested the cells in G G1 phase

modulated the protein expressions of cyclin D D1 and p27

and suppressed the activation of PKCα and ERK1/2. DHZCP containing serum inhibits VSMCs proliferation via modulating the expressions of cell cycle proteins to arrest the cell in G G1 phase

which is attributed to

at least in part

suppressing PKCα-ERK1/2 signaling in VSMCs.

Abstract

To investigate effects of Dahuang Zhechong Pill (大黄{ie371-1}虫丸

p27

protein kinase Cα (PKCα)

and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) were quantified by Western blot method. DHZCP containing serum significantly inhibited DNA synthesis of PDGF-stimulated VSMCs

arrested the cells in G G1 phase

modulated the protein expressions of cyclin D D1 and p27

and suppressed the activation of PKCα and ERK1/2. DHZCP containing serum inhibits VSMCs proliferation via modulating the expressions of cell cycle proteins to arrest the cell in G G1 phase

which is attributed to

at least in part

suppressing PKCα-ERK1/2 signaling in VSMCs.

关键词

Dahuang Zhechong Pillproliferationcell cyclecyclin D1p27

Keywords

Dahuang Zhechong Pillproliferationcell cyclecyclin D1p27

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