Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation

Yan-xiang Cheng; Rong Liu; Qin Wang; Bing-shu Li; Xue-xian Xu; Min Hu; Lu Chen; Qiong Fu; De-min Pu; Li Hong

doi:10.1007/s11655-011-0697-z

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Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation

OriginalPaper | Updated：2021-08-27

- Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation
- Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation
- 中国结合医学杂志（英文版） 2012年18卷第5期页码：359-365
- Affiliations：
  
  1. Department of Gynecology, Renmin Hospital of Wuhan University,Wuhan,China
  2. Department of Obstetrics and Gynecology, No. Air Force Hospital,Wuhan,China,161
  3. Department of Obstetrics and Gynecology, Tongji Hospital, Medical College of Wuhan University,Wuhan,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-011-0697-z
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2011-4-26，
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Cheng, Yx., Liu, R., Wang, Q. et al. Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation., Chin. J. Integr. Med. 18, 359–365 (2012). https://doi.org/10.1007/s11655-011-0697-z

Yan-xiang Cheng, Rong Liu, Qin Wang, et al. Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation[J]. Chinese Journal of Integrative Medicine, 2012,18(5):359-365.
Cheng, Yx., Liu, R., Wang, Q. et al. Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation., Chin. J. Integr. Med. 18, 359–365 (2012). https://doi.org/10.1007/s11655-011-0697-z DOI：

Yan-xiang Cheng, Rong Liu, Qin Wang, et al. Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation[J]. Chinese Journal of Integrative Medicine, 2012,18(5):359-365. DOI： 10.1007/s11655-011-0697-z.

摘要

To explore the molecular mechanism of realgar-induced apoptosis of cervical cancer cells. The cervical cancer cell line Siha was used to determine the cell viability and apoptosis after treatment with realgar using MTT assay and flow cytometry. The activities of caspase-3

-8

and -9 were detected by fluorescence resonance energy transfer technology and colorimetric assay

while the levels of Bcl-2

cytochrome c

and Bax were detected by Western blot method. Induction of apoptosis by realgar was detected in Siha cell line in a dose-dependent manner. The apoptosis was accompanied by a significant increase in cytochrome c release and activation of caspase-3 and caspase-9 but not caspase-8. Further

the realgar-induced apoptosis was inhibited by a broad-spectrum caspase inhibitor

a caspase-3 inhibitor

and a caspase-9 inhibitor but not by a caspase-8 inhibitor. Bcl-2 and Bax protein expressions were not changed by realgar. The induction of apoptosis by realgar is mediated through a cytochrome c-dependent pathway

which sequentially activates caspase-9 and caspase-3.

Abstract

-8

and -9 were detected by fluorescence resonance energy transfer technology and colorimetric assay

while the levels of Bcl-2

cytochrome c

the realgar-induced apoptosis was inhibited by a broad-spectrum caspase inhibitor

a caspase-3 inhibitor

which sequentially activates caspase-9 and caspase-3.

关键词

realgarCervical Cancerapoptosiscytochrome ccaspases

Keywords

realgarCervical Cancerapoptosiscytochrome ccaspases

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