Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells

Fu-xing Li; Shu-sheng Li

doi:10.1007/s11655-011-0700-8

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Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells

OriginalPaper | Updated：2021-08-27

- Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells
- Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells
- 中国结合医学杂志（英文版） 2012年18卷第5期页码：391-394
- Affiliations：
  
  1. Department of Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
  2. Department of Emergency, the Second Affiliated Hospital to Nanchang University,Nanchang,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-011-0700-8
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2011-4-26，
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Li, Fx., Li, Ss. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells., Chin. J. Integr. Med. 18, 391–394 (2012). https://doi.org/10.1007/s11655-011-0700-8

Fu-xing Li, Shu-sheng Li. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells[J]. Chinese Journal of Integrative Medicine, 2012,18(5):391-394.
Li, Fx., Li, Ss. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells., Chin. J. Integr. Med. 18, 391–394 (2012). https://doi.org/10.1007/s11655-011-0700-8 DOI：

Fu-xing Li, Shu-sheng Li. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells[J]. Chinese Journal of Integrative Medicine, 2012,18(5):391-394. DOI： 10.1007/s11655-011-0700-8.

摘要

To observe the effect of andrographolide on the activation of mitogen-activated protein kinases (MAPKs) and expression of nuclear factor-κB (NF-κB) in macrophage foam cells. The mouse peritoneal macrophages were cultured in the media in the presence of oxidized low-density lipoprotein (ox-LDL)

ox-LDL+andrographolide

or neither (control). The phosphorylation of MAPK molecules (p38MAPK

JNK

ERK1/2) and the expressions of NK-κB p65 were examined by Western blot. As compared with cells in the control group

the expressions of phospho-p38 and NF-κB p65 were increased in the cells cultured with either ox-LDL or ox-LDL+andrographolide (P<0.01)

but attenuated significantly in the presence of ox-LDL+ andrographolide when compared with ox-LDL (P<0.05). The phospho-JNK increased in the presence of either ox-LDL or ox-LDL+andrographolide when compared with control cells (P<0.01)

but no significant difference existed between ox-LDL and ox-LDL+andrographolide (P>0.05). The expression of phospho-ERK1/2 was increased in the presence of ox-LDL compared with the control cells (P<0.01)

but no significant differences existed between the cells cultured in the presence of ox-LDL+andrographolide and the control medium (P>0.05). Andrographolide could inhibit the activation of ERK1/2

p38MAPK and NK-κB induced by ox-LDL in macrophage foam cells

which might be one of its mechanisms in preventing atherosclerosis.

Abstract

ox-LDL+andrographolide

or neither (control). The phosphorylation of MAPK molecules (p38MAPK

JNK

ERK1/2) and the expressions of NK-κB p65 were examined by Western blot. As compared with cells in the control group

the expressions of phospho-p38 and NF-κB p65 were increased in the cells cultured with either ox-LDL or ox-LDL+andrographolide (P<0.01)

but no significant differences existed between the cells cultured in the presence of ox-LDL+andrographolide and the control medium (P>0.05). Andrographolide could inhibit the activation of ERK1/2

p38MAPK and NK-κB induced by ox-LDL in macrophage foam cells

which might be one of its mechanisms in preventing atherosclerosis.

关键词

andrographolidemouse peritoneal macrophage foam cellsmitogen activated protein kinasesenuclear factor-κBatherosclerosis

Keywords

andrographolidemouse peritoneal macrophage foam cellsmitogen activated protein kinasesenuclear factor-κBatherosclerosis

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