Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root

Ye Huang; Hui-jun Yin; Xiao-juan Ma; Jing-shang Wang; Qian Liu; Cai-feng Wu; Ke-ji Chen

doi:10.1007/s11655-011-0726-y

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Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root

OriginalPaper | Updated：2021-08-27

- Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root
- Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root
- 中国结合医学杂志（英文版） 2011年17卷第5期页码：355-360
- Affiliations：
  
  Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Key Basic Research and Development Program of China (No. 81073086)
- DOI：10.1007/s11655-011-0726-y
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-5-25，
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Huang, Y., Yin, Hj., Ma, Xj. et al. Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root, ., Chin. J. Integr. Med. 17, 355 (2011). https://doi.org/10.1007/s11655-011-0726-y

Ye Huang, Hui-jun Yin, Xiao-juan Ma, et al. Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root[J]. Chinese Journal of Integrative Medicine, 2011,17(5):355-360.
Huang, Y., Yin, Hj., Ma, Xj. et al. Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root, ., Chin. J. Integr. Med. 17, 355 (2011). https://doi.org/10.1007/s11655-011-0726-y DOI：

Ye Huang, Hui-jun Yin, Xiao-juan Ma, et al. Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root[J]. Chinese Journal of Integrative Medicine, 2011,17(5):355-360. DOI： 10.1007/s11655-011-0726-y.

摘要

To explore the correlation between Fc γ RIII A (CD16A) and aortic atherosclerotic plaque destabilization in apoE knockout (apoE KO) mice and the intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root. Eight 8-week-old male C57BL/6J mice were selected as the control group. Forty 8-week-old male apoE KO mice were randomly divided into the model group

apoE KO + intraperitoneal injection immunoglobulin group (IVIG)

apoE KO + simvastatin group (Sm)

apoE KO + high dosage of Xiongshao Capsule (XSC

芎芍胶囊) group (XSCH)

and apoE KO + low dosage of XSC group (XSCL)

8 mice in each group. Mice in the control group were put on a normal diet

and others were fed with a high-fat diet. After 10-week different interventions

monocyte CD16 expression was detected by flow cytometry

aortic matrix metalloproteinase-9 (MMP-9) mRNA expression was detected using reverse transcriptionpolymerase chain reaction

and serum tumor necrosis factor (TNF)-α level was detected using enzyme-linked immunosorbent assay. Compared with the control group

monocyte CD16 expression

aortic MMP-9 mRNA expression

and serum TNF-α level in the model group increased obviously (P<0.01). Injections of apoE KO mice with intraperitoneal immunoglobulin during a 5-day period significantly reduced the monocyte CD16 expression

aortic MMP-9 mRNA expression

and serum TNF-α level (P<0.01 or 0.05) over a 10-week period of high-fat diet. Indices above in the Sm group

XSCH group

and XSCL group decreased in a different degree. Of them

the aortic MMP-9 mRNA expression in XSCH group was lower than that in Sm group (P<0.05) and the monocyte CD16 expression and serum TNF-α level showed no significant difference between XSCH group and Sm group (P>0.05). Correlation analyses suggested positive correlation between monocyte CD16 expression and aortic MMP-9 mRNA expression or serum TNF-α level in IVIG group

XSCH group

and XSCL group. FcγR III A mediates systemic inflammation in the progression of coronary heart disease with blood stasis syndrome. XSC could stabilize atherosclerotic plaque by suppressing inflammation and its target was relative with FcγRIII A.

Abstract

apoE KO + intraperitoneal injection immunoglobulin group (IVIG)

apoE KO + simvastatin group (Sm)

apoE KO + high dosage of Xiongshao Capsule (XSC

芎芍胶囊) group (XSCH)

and apoE KO + low dosage of XSC group (XSCL)

8 mice in each group. Mice in the control group were put on a normal diet

and others were fed with a high-fat diet. After 10-week different interventions

monocyte CD16 expression was detected by flow cytometry

aortic matrix metalloproteinase-9 (MMP-9) mRNA expression was detected using reverse transcriptionpolymerase chain reaction

and serum tumor necrosis factor (TNF)-α level was detected using enzyme-linked immunosorbent assay. Compared with the control group

monocyte CD16 expression

aortic MMP-9 mRNA expression

and serum TNF-α level (P<0.01 or 0.05) over a 10-week period of high-fat diet. Indices above in the Sm group

XSCH group

and XSCL group decreased in a different degree. Of them

XSCH group

关键词

FcγRIIIAapoE knockout micematrix metalloproteinase-9tumor necrosis factor αeffective components of Chuanxiong Rhizome and Red Peony Root

Keywords

FcγRIIIAapoE knockout micematrix metalloproteinase-9tumor necrosis factor αeffective components of Chuanxiong Rhizome and Red Peony Root

references

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