Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis

Wei Liu; Yuan-hao Wu; Xiao-ya Liu; Bin Xue; Wen Shen; Kuo Yang

doi:10.1007/s11655-011-0727-x

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Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis

OriginalPaper | Updated：2021-08-27

- Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis
- Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis
- 中国结合医学杂志（英文版） 2013年19卷第6期页码：459-463
- Affiliations：
  
  1. The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin,China
  2. Tianjin University of Traditional Chinese Medicine,Tianjin,China
- Author bio：
- Funds：
  
  Supported by Practice Basic Research Plan of Tianjin City (No. 06YFJMJC09600)
- DOI：10.1007/s11655-011-0727-x
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2012-4-11，
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Liu, W., Wu, Yh., Liu, Xy. et al. Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis., Chin. J. Integr. Med. 19, 459–463 (2013). https://doi.org/10.1007/s11655-011-0727-x

Wei Liu, Yuan-hao Wu, Xiao-ya Liu, et al. Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis[J]. Chinese Journal of Integrative Medicine, 2013,19(6):459-463.
Liu, W., Wu, Yh., Liu, Xy. et al. Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis., Chin. J. Integr. Med. 19, 459–463 (2013). https://doi.org/10.1007/s11655-011-0727-x DOI：

Wei Liu, Yuan-hao Wu, Xiao-ya Liu, et al. Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis[J]. Chinese Journal of Integrative Medicine, 2013,19(6):459-463. DOI： 10.1007/s11655-011-0727-x.

摘要

To observe the metabolic

regulatory and anti-oxidative effects of modified Bushen Huoxue Decoction (BSHXD

补肾活血方)

a Chinese herbal medicine for Kidney (Shen)-reinforcement and bloodactivation

on an osteoarthritis (OA) rabbit model. A rabbit model for knee joint OA was established by the classic Hulth’s method. The OA model rabbits were randomized into 5 groups: the model control group

the positive control group treated with glucosamine sulfate

and the three BSHXD treated groups treated respectively with low

moderate

and high doses of BSHXD. In addition

a normal control group and a sham-operated group were set up. Experimental animals were sacrificed after a 7-week treatment

and pathological changes in cartilaginous tissue were estimated using the Mankin criteria. Hydroxyproline (Hyp) and malonaldehyde (MDA) contents in blood serum and urine

as well as superoxide dismutase (SOD) activity and nitric oxide (NO) content in blood serum and knee joint synovial homogenates were detected. Mankin scoring showed insignificant statistical differences between the various treatment groups (P >0.05)

but all were better than the model control group (P <0.05). Serum and urinary contents of Hyp and MDA as well as serum and synovial levels of NO were significantly lower

but the SOD activity in blood serum and synovial tissue was higher in the BSHXD treated groups than in the model group P <0.01); the effect of BSHXD was dose-dependent to some extent. The modified BSHXD shows an effect of improving cartilage metabolism in experimental rabbits with OA

and possesses osteo-chondric protective effects in antagonizing peroxidation injury.

Abstract

To observe the metabolic

regulatory and anti-oxidative effects of modified Bushen Huoxue Decoction (BSHXD

补肾活血方)

a Chinese herbal medicine for Kidney (Shen)-reinforcement and bloodactivation

on an osteoarthritis (OA) rabbit model. A rabbit model for knee joint OA was established by the classic Hulth’s method. The OA model rabbits were randomized into 5 groups: the model control group

the positive control group treated with glucosamine sulfate

and the three BSHXD treated groups treated respectively with low

moderate

and high doses of BSHXD. In addition

a normal control group and a sham-operated group were set up. Experimental animals were sacrificed after a 7-week treatment

and pathological changes in cartilaginous tissue were estimated using the Mankin criteria. Hydroxyproline (Hyp) and malonaldehyde (MDA) contents in blood serum and urine

but all were better than the model control group (P <0.05). Serum and urinary contents of Hyp and MDA as well as serum and synovial levels of NO were significantly lower

and possesses osteo-chondric protective effects in antagonizing peroxidation injury.

关键词

Kidney-reinforcing and blood-activatingosteoarthritisHydroxyprolinemalonaldehydesuperoxide dismutaseNitric Oxide

Keywords

Kidney-reinforcing and blood-activatingosteoarthritisHydroxyprolinemalonaldehydesuperoxide dismutaseNitric Oxide

references

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Liu XX, Li XH, Zhou JT. Experimental study on replicating knee osteoarthritis by modified Hulth’s modeling method. Chin J Integr Tradit West Med (Chin) 2005;25:1104–1108.

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