Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate

Xin Tong; Hui Sun; Guang-li Yan; Wei Dong; Wen-jun Sun; Yang Yu; Ping Wang; Ying Han; Xi-jun Wang

doi:10.1007/s11655-011-0728-9

Your Location：

Home >

Browse articles >

Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate

OriginalPaper | Updated：2021-08-27

- Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate
- Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate
- 中国结合医学杂志（英文版） 2011年17卷第5期页码：369-375
- Affiliations：
  
  Heilongjiang University of Traditional Chinese Medicine, Haerbin,Heilongjiang Province,China
- Author bio：
- Funds：
  
  Supported by the Key Items of the National Program for Basic Sciences Research and Development (“973” Plan No. 2005CB523406)
- DOI：10.1007/s11655-011-0728-9
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-5-25，
Scan for full text
Tong, X., Sun, H., Yan, Gl. et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate., Chin. J. Integr. Med. 17, 369–375 (2011). https://doi.org/10.1007/s11655-011-0728-9

Xin Tong, Hui Sun, Guang-li Yan, et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate[J]. Chinese Journal of Integrative Medicine, 2011,17(5):369-375.
Tong, X., Sun, H., Yan, Gl. et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate., Chin. J. Integr. Med. 17, 369–375 (2011). https://doi.org/10.1007/s11655-011-0728-9 DOI：

Xin Tong, Hui Sun, Guang-li Yan, et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate[J]. Chinese Journal of Integrative Medicine, 2011,17(5):369-375. DOI： 10.1007/s11655-011-0728-9.

摘要

To ascertain the biomarkers capable to characterize the animal composite model of Chinese medicine (CM) Yinhuang (阴黄) syndrome induced by triplet factors of rhubarb

ethanol

and α-nephthylisothiolyanate (abbreviated as R

and A below) through metabolomic study and to evaluate the established model by means of studying the sources of markers based on the changes of metabolites produced from various combinations of the three modeling drugs. Eighty Wistar rats allocated equally in eight groups (A–H) were treated with saline

R+E+A

R+E

R+A

and E+A

respectively. Rats’ 12 h urine in the 14 successive experimental days were collected separately using metabolic cages and analyzed by ultra peformance liquid chromatograph/time of flight mass spectrometer (UPLC/TOF-MS) to create the metabolic contour graph of urine in different groups for identifying the differences between them. The similarities and differences of metabolic network among various groups were represented from microcosmic viewpoint by pattern recognition method (principal component analysis). Controlled by group A

the landing points in principal component map of various groups were apparently assorted

especially obvious on the 14th day; 19 biomarkers

which capable to represent the genesis and development process of the Yinhuang syndrome in the triplet factors-induced rat model

were identified. Metabolomic method is successfully used in evaluating the animal model of CM syndrome. Furthermore

according to the holistic view and substance changes in vivo

the influences of disease on organism were comprehensively analyzed

and the pathogenic mechanism of CM Yinhuang syndrome was explored at the level of metabolomics in vivo as well.

Abstract

To ascertain the biomarkers capable to characterize the animal composite model of Chinese medicine (CM) Yinhuang (阴黄) syndrome induced by triplet factors of rhubarb

ethanol

and α-nephthylisothiolyanate (abbreviated as R

R+E+A

R+E

R+A

and E+A

the landing points in principal component map of various groups were apparently assorted

especially obvious on the 14th day; 19 biomarkers

which capable to represent the genesis and development process of the Yinhuang syndrome in the triplet factors-induced rat model

were identified. Metabolomic method is successfully used in evaluating the animal model of CM syndrome. Furthermore

according to the holistic view and substance changes in vivo

the influences of disease on organism were comprehensively analyzed

and the pathogenic mechanism of CM Yinhuang syndrome was explored at the level of metabolomics in vivo as well.

关键词

rhubarbethanolα-nephthylisothiolyanateYinhuang syndromeanimal modelmetabolomics

Keywords

rhubarbethanolα-nephthylisothiolyanateYinhuang syndromeanimal modelmetabolomics

references

Liu L, Sun H, Wang XJ, Wang P, Wu ZM, LU HT, et al. Hepatoprotection of YinChenHaoTang on acute rat liver injury induced by ethanol. World Sci Technol /Mod Tradit Chin Med Mater Med (Chin) 2007;9:35–40.

Stella C, Beckwith-Hall B, Cloarec O, Holmes E, Lindon JC, Powell J, et al. Susceptibility of human metabolic phenotypes to dietary modulation. J Proteome Res 2006;5:2780–2788.

Dumas ME, Barton RH, Toye A, Cloarec O, Blancher C, Rothwell A, et al. Metabolic profiling reveals a contribution of gut microbiota to fatty liver phenotype in insulin-resistant mice. Proc Natl Acad Sci USA 2006;103:12511–12516.

Zhang CZ, Zeng H, Cheng LB. Establish and significance of the Yin Jaundice paterner animal model. Chin J Basic Med Tradit Chin Med (Chin) 2000;6:43–44.

Lu JB, Zhang CZ, Yan XS. A study of apoptosis and gene regulation in peripheral blood lymphocytes of Yin Jaundice. Chin J Integr Tradit West Med Liver Dis (Chin) 2002;12:150–152.

Tian JZ, Wang YY, Shi J. Research thinking on syndrome model defined by traditional Chinese medicine. J Beijing Univers Tradit Chin Med (Chin) 2005;28:18–21.

Wan SH, Ding YQ, Pu XH, Guo L, Kang TG. Protective effect of Hydroxyan throquinones from Rheum Palmatum L on acute liver injure of rats induced by CCl4. Chin Pharmacol Bull (Chin) 2006;22:1405–1406.

Nicholson JK, Lindon JC, Holmes E. Metabonomics: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data. Xeno Biotica 1999;29:1181–1189.

He J, Zhou HH. Metabonomics and its growing in sights into pharmacology. Chin Phamacol Bull (Chin) 2006;22S:1304–1309.

Wang XJ, Liu L, Sun H, Lu HT. Studieson themetabonomics of rat liver injury induced by ethanol and interfering efects of YinChenHaoTang. Chin Pharmacol Bull (Chin) 2008;24:452–457.

Nicholson JK, Connelly J, Lindon JC, Holmes E. Metabonomics: aplatform for studying drug toxicity and gene function. Nat RevDrug Discov 2002;1:153–161.

FULL TEXT LINK

More>

浏览量

742

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Application of metabolomics technology in the research of Chinese medicine

Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress

A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector

Effect of Fufang Xueshuantong Capsule (复方血栓通胶囊) on a rat model of retinal vein occlusion

Thinking path and method for establishing the disease/Chinese medicine syndrome conjugated pattern of a mammary hyperplasia animal model