Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes

Ming-xue Zhang; Yu-mei Zhang; Jun Esther; Wei He; Hong-hua Che; Ping Gu

doi:10.1007/s11655-011-0729-8

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Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes

OriginalPaper | Updated：2021-08-27

- Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes
- Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes
- 中国结合医学杂志（英文版） 2011年17卷第5期页码：376-380
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang,China
  2. School of Oriental Medicine, Health Center, Life University, Marietta,USA
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30873399);Doctoral Funds from Ministry of Education (No. 20070162002);and Excellent Talents in Universities Support Plan of Liaoning Province
- DOI：10.1007/s11655-011-0729-8
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-5-25，
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Zhang, Mx., Zhang, Ym., Esther, J. et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes., Chin. J. Integr. Med. 17, 376–380 (2011). https://doi.org/10.1007/s11655-011-0729-8

Ming-xue Zhang, Yu-mei Zhang, Jun Esther, et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes[J]. Chinese Journal of Integrative Medicine, 2011,17(5):376-380.
Zhang, Mx., Zhang, Ym., Esther, J. et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes., Chin. J. Integr. Med. 17, 376–380 (2011). https://doi.org/10.1007/s11655-011-0729-8 DOI：

Ming-xue Zhang, Yu-mei Zhang, Jun Esther, et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes[J]. Chinese Journal of Integrative Medicine, 2011,17(5):376-380. DOI： 10.1007/s11655-011-0729-8.

摘要

To study the effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes

to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of Yiqi Huoxue Recipe on gene level

and to further investigate whether Yiqi Huoxue Recipe is an effective prescription for CVB3 myocarditis. A modified suppression subtractive hybridization (SSH) was used to isolate differentially expressed genes between the CVB3 infection group and the treatment group with Yiqi Huoxue Recipe. The results were further verified by fluorescence RT-PCR. The results of SSH showed that the gene expression of ribosomal protein S20 in the treatment group was higher than that in the CVB3 infection group (P<0.05)

which agreed with the results of fluorescent RT-PCR. Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis

and Yiqi Huoxue Recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20.

Abstract

To study the effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes

to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of Yiqi Huoxue Recipe on gene level

which agreed with the results of fluorescent RT-PCR. Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis

and Yiqi Huoxue Recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20.

关键词

Viral MyocarditisYiqi Huoxue recipesuppression subtractive hybridizationribosomal protein S20

Keywords

Viral MyocarditisYiqi Huoxue recipesuppression subtractive hybridizationribosomal protein S20

references

Zhang Y, Zhu H, Huang C, Cui X, Gao Y, Huang Y, et al. Astragaloside IV exerts antiviral effects against coxsackievirus B3 by upregulating interferon-gamma. J Cardiovasc Pharmacol (Chin) 2006;47:190–195.

Martherus RS, Zeijlemaker VA, Ayoubi TA. Electrical stimulation of primary neonatal rat ventricular cardiomyocytes using pacemakers. Biotechniques 2010;48:65–67.

Formigli L, Francini F, Nistri S, Margheri M, Luciani G, Naro F, et al. Skeletal myoblasts overexpressing relaxin improve differentiation and communication of primary murine cardiomyocyte cell cultures. J Mol Cell Cardiol 2009;47:335–345.

Shen Y, Xu W, Chu YW, Wang Y, Liu QS, Xiong SD. Coxsackie virus group B type 3 infection upregulates expression of monocyte chemoattractant protein 1 in cardiac myocytes, which leads to enhanced migration of mononuclear cells in viral myocarditis. J Virol (Chin) 2004;78:12548–12556.

Zhang Y, Zhu H, Ye G, Huang C, Yang Y, Chen R, et al. Antiviral effects of sophoridine against coxsackievirus B3 and its pharmacokinetics in rats. Life Sci 2006;78:1998–2005.

Wool IG. The structure and function of eukaryotic ribosomes. Annu Rev Biochem 1979;48:719–754.

Wittmann HG. Structure and evolution of ribosomes. Mol Biol Biochem Biophys 1980;32:376–397.

Brimacombe R, Stiege W. Structure and function of ribosomal RNA. Biochem J 1985;229:1–17.

Wittmann-Liebold B, Marzinzig E, Lehmann A. Primary structure of protein S20 from the small ribosomal subunit of Escherichia coli. FEBS Lett 1976;68:110–114.

Weber HJ. Stoichiometric measurements of 30S and 50S ribosomal proteins from Escherichia coli. Mol Gen Genet 1972;119:233–248.

Kurland CG. Ribosome structure and function emergent. Science 1970;169:1171–1177.

Ungewickell E, Garrett R, Ehresmann C, Stiegler P, Fellner P. An investigation of the 16-S RNA binding sites of ribosomal proteins S4, S8, S15, and S20 from Escherichia coli. Eur J Biochem 1975;51:165–180.

Ehresmann B, Backendorf C, Ehresmann C, Ebel JP. Characterization of the regions from E. coli 16 S RNA covalently linked to ribosomal proteins S4 and S20 after ultraviolet irradiation. FEBS Lett 1977;78:261–266.

Chu W, Presky DH, Swerlick RA, Burns DK. Human ribosomal protein S20 cDNA sequence. Nucleic Acids Res 1993;2:1672.

Goldstone SD, Lavin MF. Isolation of a cDNA clone, encoding the ribosomal protein S20, downregulated during the onset of apoptosis in a human leukaemic cell line. Biochem Biophys Res Commun 1993;196:619–623.

Kemp EH, Herd LM, Waterman EA, Wilson AG, Weetman AP, Watson PP. De Bortoli M, Castellino RC, Lu XY, Deyo J, Sturla LM, Adesina AM, et al. Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8. BMC Cancer 2006;6:223.

De Bortoli M, Castellino RC, Lu XY, Deyo J, Sturla LM, Adesina AM, et al. Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8. BMC Cancer 2006;6:223.

McGowan KA, Li JZ, Park CY, Beaudry V, Tabor HK, Sabnis AJ, et al. Ribosomal mutations cause p53-mediated dark skin and pleiotropic effects. Nat Genet 2008;40:963–970.

Waxman S, Wurmbach E. De-regulation of common housekeeping genes in hepatocellular carcinoma. BMC Genomics 2007;8:243.

Bugger H, Leippert S, Blum D, Kahle P, Barleon B, Marme D, et al. Subtractive hybridization for differential gene expression in mechanically unloaded rat heart. Am J Physiol Heart Circ Physiol 2006;291:2714–2722.

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