Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia

Hao Li; Lin-lin Cai; Jian-gang Liu; Long-tao Liu; Jie Guan; Ming-fang Liu; Jia Hu; Yun Wei

doi:10.1007/s11655-011-0752-9

Your Location：

Home >

Browse articles >

Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia

OriginalPaper | Updated：2021-08-27

- Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia
- Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia
- 中国结合医学杂志（英文版） 2011年17卷第6期页码：430-435
- Affiliations：
  
  1. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Endocrinal Department of Haici Medical Group,Shandong Province,Qingdao,China
  3. National Key Laboratory for Available Utilization of Chemical Engineering Resource, Beijing Chemical Engineering University,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30873338);and the National Major Special Project of Science and Technology (No. 2009ZX09103-391)
- DOI：10.1007/s11655-011-0752-9
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-6-10，
Scan for full text
Li, H., Cai, Ll., Liu, Jg. et al. Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia., Chin. J. Integr. Med. 17, 430–435 (2011). https://doi.org/10.1007/s11655-011-0752-9

Hao Li, Lin-lin Cai, Jian-gang Liu, et al. Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia[J]. Chinese Journal of Integrative Medicine, 2011,17(6):430-435.
Li, H., Cai, Ll., Liu, Jg. et al. Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia., Chin. J. Integr. Med. 17, 430–435 (2011). https://doi.org/10.1007/s11655-011-0752-9 DOI：

Hao Li, Lin-lin Cai, Jian-gang Liu, et al. Effect of early intervention with extract of Huannao Yicong Decoction (还脑益聪方) on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia[J]. Chinese Journal of Integrative Medicine, 2011,17(6):430-435. DOI： 10.1007/s11655-011-0752-9.

摘要

To observe the effect of early intervention using extract of Huannao Yicong Decoction (还脑益聪方

HYD) on the pathological picture of hippocampus

neurocyte apoptosis

and associated regulatory genes in β-amyloid precursor protein (APP) transgenic mice model of dementia. Sixty APP695V7171 transgenic mice were randomly divided into four groups of 15. The model group was treated with distilled water

the positive control group was treated with donepezil (0.65 mg/kg)

and the two HYD groups were treated with high dose (2.8 g/kg) and low dose (1.4 g/kg) HYD

respectively. All testing drugs were administered through gastrogavage by dissolving in equal volume of distilled water

once a day for six successive months. In addition

a normal control group with 15 healthy C57BL/6J mice of the same age and genetic background was set up with distilled water treatment. The pathologic picture of brain tissue was observed by microscopy with HE stain; the amount of apoptosis cells in the hippocampal CA1 area was detected by TUNEL; and expressions of associated genes

Bcl-2

and Bax were determined by immunohistochemical method. Pathologic pictures of hippocampus showed that in the model group

cells messily arranged

neurons markedly decreased

and the surrounding tissue of some cells was loosened with edema

necrosis

and widened gap with glia cells proliferation. Compared with those in the normal group

the amount of apoptosis cells in the CA1 area was increased

Bcl-2 expression decreased

and Bax expression increased significantly

with markedly reduced Bcl-2/Bax ratio in the model group. Compared to the model group

the pathological changes were significantly milder in the HYD-treated groups

showing rather regularly arranged cells

significantly increased neurons

only few denatured necrotic cells with milder edema

less proliferation of glia cells

and obviously reduced cell apoptosis in CA1 area (P<0.05 or P<0.01). Besides

Bcl-2 expression was up-regulated and Bax expression down-regulated

and Bcl-2/Bax ratio significantly increased in the two HYD groups (P<0.05 or P<0.01). Early intervention with HYD could improve the abnormal pathologic picture of hippocampus and regulate the expressions of associated genes to suppress cell apoptosis

which might be its mechanism of action in alleviating cognitive functional disorder.

Abstract

To observe the effect of early intervention using extract of Huannao Yicong Decoction (还脑益聪方

HYD) on the pathological picture of hippocampus

neurocyte apoptosis

the positive control group was treated with donepezil (0.65 mg/kg)

and the two HYD groups were treated with high dose (2.8 g/kg) and low dose (1.4 g/kg) HYD

respectively. All testing drugs were administered through gastrogavage by dissolving in equal volume of distilled water

once a day for six successive months. In addition

Bcl-2

and Bax were determined by immunohistochemical method. Pathologic pictures of hippocampus showed that in the model group

cells messily arranged

neurons markedly decreased

and the surrounding tissue of some cells was loosened with edema

necrosis

and widened gap with glia cells proliferation. Compared with those in the normal group

the amount of apoptosis cells in the CA1 area was increased

Bcl-2 expression decreased

and Bax expression increased significantly

with markedly reduced Bcl-2/Bax ratio in the model group. Compared to the model group

the pathological changes were significantly milder in the HYD-treated groups

showing rather regularly arranged cells

significantly increased neurons

only few denatured necrotic cells with milder edema

less proliferation of glia cells

and obviously reduced cell apoptosis in CA1 area (P<0.05 or P<0.01). Besides

Bcl-2 expression was up-regulated and Bax expression down-regulated

which might be its mechanism of action in alleviating cognitive functional disorder.

关键词

β-amyloid precursor protein transgenic miceextract of Huannao Yicong Decoctioncell apoptosis

Keywords

β-amyloid precursor protein transgenic miceextract of Huannao Yicong Decoctioncell apoptosis

references

Jellinger KA. Challenges in neuronal apoptosis. Curr Alzheimer Res 2006;3:377–391.

Blennow K, de Leon MJ, Zetterberg H. Alzheimer’s disease. Lancet 2006;368:387–403.

Li H, Liu F, Xu LR, Hou LF, Cui L, Luo ZG. Effect of Huannao Yicong Capsule on senile mild cognitive impairment. Chin J Integr Tradit West Med (Chin) 2004; 24:689–693.

Li H, Yao MJ, Zhao WM, Guan J, Cai LL, Cui L. A randomized, controlled, double-blind trial of Huannao Yicong Capsule in senile patients with mild cognitive impairment. J Chin Integr Med (Chin) 2008;6:25–31.

Li H, Yao MJ, Li X, Liu JG, Zhao WM. Effects of Huannao Yicong Decoction on cognitive function and expressions of Bcl-2 and Bax protein of hippocampus in cognitive impairment rats. Chin J Chin Mater Med (Chin) 2009;34:2622–2626.

Sheng SL. Research progress on Alzheimer’s disease: pathogenesis and medical therapy. Acta Acad Med Sin 2004;26:101–103.

Wilcock DM, Carol A, Colton J. Anti-Aβ immunotherapy in Alzheimer’s disease: relevance of transgenic mouse studies to clinical trials. Alzheimers Dis 2008;15:555–569.

Duyckaerts C, Potier MC, Delatour B. Alzheimer disease models and human neuropathology: similarities and differences. Acta Neuropathol 2008;115:5–38.

Qin C, Chang Y, Zhang BL, Zhu H, Yin HX, Gao H. The pathological study on spontaneous disease of APP transgenic mice. Chin J Lab Anim Sci (Chin) 2001;11:27–29.

Li H, Liu JG, Yao MJ, Zhao WM. Effect of Huannao Yicong Prescription on AD rat in learning and memory and hippocampus of Aβ-related protein expression with lipid metabolism. Chin J Basic Med Tradit Chin Med (Chin) 2009;15:354–357.

Yao MJ, Li H, Zhao WM, Liu JG, Guan J. Effect of Huannao Yicong Formula on pathomorphology, inflammatory factor and oxidative stress index of hippocampus in cognitive impairment rats at early stage. World J Integr Tradit West Med (Chin) 2010;5:300–304.

Li H, Zhang GX, Tu RS, Liu L. Effect of Huannao Yicong Prescription on AD rat in ethology, hippocamp tissue pahomorphology and blood rheology. Chin J Hemorrheol (Chin) 2008;18:4–7.

Luo XL, Mao M. The molecular mechanism of signal transduction of neurotrophins and apoptosis of neurocyte. J Bio Med Eng (Chin) 2007;24:1187–1190.

Calissano P, Matrone C, Amadoro G. Apoptosis and in vitro Alzheimer disease neuronal models. Commun Integr Biol 2009;2:163–169.

Yang Y, Varvel NH, Lamb BT, Herrup K. Ectopic cell cycle events link human Alzheimer’s disease and amyloid precursor protein transgenic mouse models. J Neurosci 2006;26:775–784.

Liu MF, Li H, Liu JG, Liu LT. The relationship between the deficiency, stasis, turbidity and toxin of traditional Chinese medicine and senile dementia. J Tradit Chin Med (Chin) 2010;51:651–654.

Cai LL, Li H, Liu JG, Liu LT, Guan J, Liu MF. Effects of early intervention with Huannao Yicong Fang effective fractions on behavior and cholinergic system of β-amyloid precursor protein transgenic dementia model. J Chin Integr Med (Chin) 2011;9:292–298.

FULL TEXT LINK

More>

浏览量

524

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression

Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum

Progress in research on the anti-tumor effect of artesunate

Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis