Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis

Xi-de Liu; Ying Chen; Feng-yun Liu; Li-hong Ye; Long Cai

doi:10.1007/s11655-011-0753-8

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Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis

OriginalPaper | Updated：2021-08-27

- Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis
- Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis
- 中国结合医学杂志（英文版） 2013年19卷第6期页码：453-458
- Affiliations：
  
  1. Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine,Hangzhou,China
  2. Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the Zhejiang Provincial Project of Traditional Chinese Medicine Science and Technology Plan (No. 2007CB195, 2008CA086)
- DOI：10.1007/s11655-011-0753-8
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2011-6-29，
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Liu, Xd., Chen, Y., Liu, Fy. et al. Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis., Chin. J. Integr. Med. 19, 453–458 (2013). https://doi.org/10.1007/s11655-011-0753-8

Xi-de Liu, Ying Chen, Feng-yun Liu, et al. Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis[J]. Chinese Journal of Integrative Medicine, 2013,19(6):453-458.
Liu, Xd., Chen, Y., Liu, Fy. et al. Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis., Chin. J. Integr. Med. 19, 453–458 (2013). https://doi.org/10.1007/s11655-011-0753-8 DOI：

Xi-de Liu, Ying Chen, Feng-yun Liu, et al. Effect of wenhua juanbi recipe (温化蠲痹方) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis[J]. Chinese Journal of Integrative Medicine, 2013,19(6):453-458. DOI： 10.1007/s11655-011-0753-8.

摘要

To study the effect of Wenhua Juanbi Recipe (温化蠲痹方

WJR) on proliferation and apoptosis of synoviocytes in rats with collagen-inducing arthritis (CIA). A CIA model was induced by intradermal injection of bovine collagen type II emulsion at the base of rat tails. Thirty modeled healthy Wistar rats were randomly assigned to one of three groups (10 per group): the model group

the methotrexate (MTX)-treated group (0.78 mg/kg) and the WJR-treated group (22.9 g/kg). A group of 10 healthy rats was used as normal control. Treatments or normal saline for the control group were administered by oral gavage once daily. Rats were sacrificed after 30-day treatment and subjected to the following examinations: arthritis index (AI) was estimated

inflammatory cell infiltration and proliferation in synovial membrane were evaluated by microscopy

the synoviocyte apoptosis was determined by TUNEL assay

and the cell apoptosis index was calculated. AI was lowered significantly in the WJR group compared to the model group (P<0.01). The pathological findings observed in the model group were reversed in the WJR group

including increase in inflammatory cell infiltration and synoviocyte proliferation in synovial membrane and reduction in cell apoptosis index (all P<0.01). Synoviocyte proliferation and apoptosis reduction were present in CIA rats. WJR was effective in treating the rat model of CIA. The therapeutic effect might be exerted through inducing apoptosis and suppressing proliferation of synoviocytes.

Abstract

To study the effect of Wenhua Juanbi Recipe (温化蠲痹方

inflammatory cell infiltration and proliferation in synovial membrane were evaluated by microscopy

the synoviocyte apoptosis was determined by TUNEL assay

关键词

Wenhua Juanbi Recipecollagen-induced arthritiscell proliferationapoptosissynoviumrat

Keywords

Wenhua Juanbi Recipecollagen-induced arthritiscell proliferationapoptosissynoviumrat

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