Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats

Yan Luo; Neng-gui Xu; Wei Yi; Tao Yu; Zhong-hua Yang

doi:10.1007/s11655-011-0754-7

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Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats

OriginalPaper | Updated：2021-08-27

- Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats
- Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats
- 中国结合医学杂志（英文版） 2011年17卷第10期页码：750-757
- Affiliations：
  
  1. Institute of Acupuncture and Moxibustion, Guangxi College of Traditional Chinese Medicine,Nanning,China
  2. College of Acupuncture & Moxibustion and Massage, Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
  3. Depertment of Acupuncture & Moxibustion, Guangdong Province Hospital of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program, No. 2010CB530500);National Natural Science Foundation of China (No. 30572420);Program for New Century Excellent Talents in University, Ministry of Education of China (No. NCET-04-0831)
- DOI：10.1007/s11655-011-0754-7
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2013-3-26，
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Luo, Y., Xu, Ng., Yi, W. et al. Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats., Chin. J. Integr. Med. 17, 750–757 (2011). https://doi.org/10.1007/s11655-011-0754-7

Yan Luo, Neng-gui Xu, Wei Yi, et al. Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats[J]. Chinese Journal of Integrative Medicine, 2011,17(10):750-757.
Luo, Y., Xu, Ng., Yi, W. et al. Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats., Chin. J. Integr. Med. 17, 750–757 (2011). https://doi.org/10.1007/s11655-011-0754-7 DOI：

Yan Luo, Neng-gui Xu, Wei Yi, et al. Study on the correlation between synaptic reconstruction and astrocyte after ischemia and the influence of electroacupuncture on rats[J]. Chinese Journal of Integrative Medicine, 2011,17(10):750-757. DOI： 10.1007/s11655-011-0754-7.

摘要

To observe the effects of electroacupuncture (EA) on the structure parameters of synapse and reactive changes of astrocyte in the marginal zone of focal cerebral ischemia in rats at different time zones so as to further explore its underlying mechanisms in the treatment of cerebral ischemia. Ninety male Wistar rats were randomly assigned to sham-operation

model

and EA groups

with 30 animals in each group. Each group was subdivided into 1 h

as well as 1

and 21 days post-operation groups

with 6 animals assigned to each time point subgroup. Heat coagulation-induced occlusion of the middle cerebral artery was performed to establish a model of focal cerebral ischemia. EA was applied immediately following surgery to the EA group [4/20 Hz

2.0–3.0 V

1–3 mA

to Baihui (GV20) and Dazhui (GV14)] for 30 min. Treatment was performed once a day

and experimental animals were sacrificed at 1 h

as well as 1

7 and 21 days postoperation. The ultrastructure changes in synapse and astrocytes were observed by using transmission electron microscopy. Glial fibrillary acidic protein (GFAP) expression and Ca2+ of astrocytes were measured by using laser confocal scanning microscope. Excitatory amino acid transporters-2 (EAAT2) and connexin 43 (CX43) expressions were assayed with immunohistochemical method. Canonical correlation analysis was conducted between structure parameters of synapse and parameters of astrocyte in the same time and group. Broken synapses were observed following cerebral ischemia

and the numbers of synapses were significantly decreased. Compared with the model group

synaptic ultrastructure was significantly improved in the EA group. Compared with the sham-operation group

synaptic number density was significantly decreased

as were postsynaptic density thickness

synaptic cleft width and synaptic interface curvature in the EA and model groups. However

compared with the model group

postsynaptic density thickness was significantly increased in the EA group at the same time points post-operation (P <0.05

P<0.01). In addition

synaptic cleft width

synaptic number density and synaptic interface curvature were significantly increased with the passage of time (P <0.05

P<0.01). The expression of GFAP in the EA group were significantly lower than those in the model group at all the time points (P <0.05

P<0.01). OD values of EAAT2 in the EA group were significantly higher than those in the model group at the same time (P <0.05

P<0.01). Compared with that in the model group

the expressions of CX43 in the EA group increased significantly at 3 days and 7 days (P <0.05

P<0.01). Ca2+ average fluorescence intensity of astrocytes in the EA group was significantly lower than those in the model group at 1 h

1 day

3 days and 7 days (P <0.05

P<0.01). The changes in structure parameters of synapse were closely related to the changes of CX43

EAAT2

GFAP

Ca2+ of astrocytes by EA treatment at all the time points. EA is helpful for synaptic reorganization

which may be related to its effect on intervening the activation state of astrocytes and promoting the beneficial interaction between astrocytes and synapses. Acupuncture could start the adjustment of neuron-glial network so as to promote the synaptic reorganization

which may be the key mechanism of treating cerebral ischemia.

Abstract

model

and EA groups

with 30 animals in each group. Each group was subdivided into 1 h

as well as 1

and 21 days post-operation groups

2.0–3.0 V

1–3 mA

to Baihui (GV20) and Dazhui (GV14)] for 30 min. Treatment was performed once a day

and experimental animals were sacrificed at 1 h

as well as 1

and the numbers of synapses were significantly decreased. Compared with the model group

synaptic ultrastructure was significantly improved in the EA group. Compared with the sham-operation group

synaptic number density was significantly decreased

as were postsynaptic density thickness

synaptic cleft width and synaptic interface curvature in the EA and model groups. However

compared with the model group

postsynaptic density thickness was significantly increased in the EA group at the same time points post-operation (P <0.05

P<0.01). In addition

synaptic cleft width

synaptic number density and synaptic interface curvature were significantly increased with the passage of time (P <0.05

P<0.01). The expression of GFAP in the EA group were significantly lower than those in the model group at all the time points (P <0.05

P<0.01). OD values of EAAT2 in the EA group were significantly higher than those in the model group at the same time (P <0.05

P<0.01). Compared with that in the model group

the expressions of CX43 in the EA group increased significantly at 3 days and 7 days (P <0.05

P<0.01). Ca2+ average fluorescence intensity of astrocytes in the EA group was significantly lower than those in the model group at 1 h

1 day

3 days and 7 days (P <0.05

P<0.01). The changes in structure parameters of synapse were closely related to the changes of CX43

EAAT2

GFAP

Ca2+ of astrocytes by EA treatment at all the time points. EA is helpful for synaptic reorganization

which may be the key mechanism of treating cerebral ischemia.

关键词

electroacupunctureischemiabrain plasticitysynaptic reorganizationastrocyterat

Keywords

electroacupunctureischemiabrain plasticitysynaptic reorganizationastrocyterat

references

Zhu YL, ed. Brain plasticity and functional reorganization. Chin J Intern Med (Chin) 2000;39:567–568.

Wang XZ, Shen DG. Evaluation of comprehensive technology on cerebral function and brain plasticity. Foreign Med Sci: Phys Med Rehabil (Chin) 2005;25(2):49–53.

Benarroch EE. Neuron-astrocyte interactions: partnership for normal function and disease in the central nervous system. Mayo Clin Proc 2005;80:1326–1338.

Horiuchi M, Tomooka Y. An attempt to generate neurons from an astrocyte progenitor cell line FBD-104. Neurosci Res 2005;53:104–115.

Seifert G, Schilling K, Steinhäuser C. Astrocyte dysfunction in neurological disorders: a molecular perspective. Nat Rev Neurosci 2006;7:194–206.

Baranano DE, Ferris CD, Snyder SH. Atypical neural messengers. Trends Neurosci 2001;24:99–106.

Yang XY, Li Z, Qin LY, Yu CH. Astrocytes and neurons glutamate-glutamine metabolism coupling. Progr Physiol Sci (Chin) 2003;34:350–352.

Mauch DH, Nägler K, Schumacher S, Göritz C, Müller EC, Otto A, et al. CNS synaptogenesis promoted by glia-derived cholesterol. Science 2001;294:1354–1357.

van der Hel WS, Notenboom RG, Bos IW, van Rijen PC, van Veelen CW, de Graan PN. Reduced glutamine synthetase in hippocampal areas with neuron loss in temporal lobe epilepsy. Neurology 2005;64:326–333.

Xu NG, Yi W, Ma QY, Xu GS, Zhu SL, Chen QZ. A study on the effect of electroacupuncture on injured neuronal protection after focal cerebral ischemia in rats. Chin Acupunct Moxibust (Chin) 2000;20:237–240.

Lin WZ, Wang P, eds. Experimental acupuncture. Shanghai: Shanghai Science and Technology Press; 1994:286–290.

Bederson JB, Pitts LH, Tsuji M, Nishimura MC, Davis RL, Bartkowski H. Rat middle cerebral artery occlusion: evaluation of the model and deveolpment of a neurologic examination. Stroke 1986;17:472–476.

Xu NG, Yi W, Lai XS, Jin R. Effect of electroacupuncture on calcium content in neurocytes of focal cerebral ischemia. Chin J Integr Tradit West Med (Chin) 2002;22:295–297.

Xu NG, Wang GB, Yi W, She SF, Huang ZY, Lai XS. Effects of electroacupuncture on the expression of cortical P38 and GAP-43 in topical cerebral ischemia rats. Acupunct Res (Chin) 2004;29(2):85–89.

Xu NG, Wang GB, She SF, Yi W, Lai XS. Influence of electroacupuncture on expression of brain-derived neurotrophic factor in ischemic cortex of rats with cerebral ischemia. J Guangzhou Univ Chin Med (Chin) 2004;21:439–442.

Wang GB, Xu NG, She SF, Yi W, Huang ZY, Lai XS. Effect of electroacupuncture on the synaptic structure in the ischemic areas of rats with focal cerebral ischemia. Chin J Clin Rehabil (Chin) 2005;9(5):115–117.

Kolb B, Whishaw Q. Brain plasticity and behavior. Annu Rev Psychol 1998;49:43–64.

Rijntjes M, Weiller C. Recovery of motor and language abilities after stroke: the contribution of functional imaging. Progr Neurobiol 2002;66:109–122.

Tang Y, Yu SG, Chen J. Enhanced synapstic plasticity in Parkinson’s disease mice sunbstantla nigra by electroacupuncture therapy. J Chengdu Univ Tradit Chin Med (Chin) 2005;28:29–32.

Luo S, Yu SG, Han T. Effects of electroacupuncture on synaptic plasticity of hippocampal neurons in model rats with Alzheimer’s disease. Chin J Clin Rehabil (Chin) 2006;10(27):187–189.

Yan B, He LL, Xu NG, Tang Y, Tang ChZ, Shao Y. Influences of electro-acupuncture on learning and memory abilities and ultrastructure of gray type I synapse in CA3 sector of the hippocampus in vascular dementia rats. Chin J Clin Rehabil (Chin) 2006;10(39):42–45.

Xu ZH, Xu NG, Yi W, FU WB, Jin R. The improvement of synaptic plasticity in the rat dentate gyrus after stroke by acupuncture. J Anhui Tradit Chin Med (Chin) 2007;26(3):18–23.

Kajihara H, Tsutsumi E, Kinoshita A, Nakano J, Takagi K, Takeo S. Activated astrocytes with glycogen accumulation in ischemic penumbra during the early stage of brain infarction: immunohistochemical and electron microscopic studies. Brain Res 2001;909:92–101.

Xiao B, Xu M. Progress in research of the central nervous system gap junctions. Foreign Med Sci: Physiol, Pathol Clin (Chin) 2002;22:205–208.

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