Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats

Zhi-kui Chen; Li-wu Lin; Jing Cai; Fa-duan Yang; Hua-jing Cai; En-sheng Xue; Jing Huang; Hong-fen Wei; Xiu-juan Zhang

doi:10.1007/s11655-011-0757-4

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Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats

OriginalPaper | Updated：2021-08-27

- Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats
- Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats
- 中国结合医学杂志（英文版） 2013年19卷第1期页码：48-53
- Affiliations：
  
  1. Ultrasonic Department, Affiliated Union Hospital of Fujian Medical University,Fuzhou,China
  2. Department of Pathology, Affiliated Xiehe Hospital of Fujian Medical University,Fuzhou,China
  3. Integrative Medical Institute of Fujian University of Traditional Chinese Medicine,Fuzhou,China
  4. Department of Pharmacology, College of Pharmacy, Fujian Medical University,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Fujian Provincial Funds of Natural Sciences for Creative Items of Youth (No. 2009J05066)
- DOI：10.1007/s11655-011-0757-4
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2011-6-29，
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Chen, Zk., Lin, Lw., Cai, J. et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats., Chin. J. Integr. Med. 19, 48–53 (2013). https://doi.org/10.1007/s11655-011-0757-4

Zhi-kui Chen, Li-wu Lin, Jing Cai, et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats[J]. Chinese Journal of Integrative Medicine, 2013,19(1):48-53.
Chen, Zk., Lin, Lw., Cai, J. et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats., Chin. J. Integr. Med. 19, 48–53 (2013). https://doi.org/10.1007/s11655-011-0757-4 DOI：

Zhi-kui Chen, Li-wu Lin, Jing Cai, et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats[J]. Chinese Journal of Integrative Medicine, 2013,19(1):48-53. DOI： 10.1007/s11655-011-0757-4.

摘要

To explore the effect of ricin temperature response gel on breast cancer and its regulatory effect on immune function in rats. Ricin was purified by chromatography and identified by immunoblotting. The rat subcutaneously transplanted breast cancer model was established. Forty model rats with a tumor diameter of about 3.0 cm were subjected to the study. They were randomized into four groups equally: the model group and three treated groups (blank gel

ricin

ricin-gel) were administered with blank gel

ricin

and ricin temperature response gel via percutaneous intratumor injection

respectively. The tumor was isolated 10 days later for the estimation of tumor inhibition rate (TIR) by weighing

pathologic examination

and detection of tumor apoptosis-associated genes bcl-2 and bax with semiquantitative RT-PCR. Also

peripheral blood was obtained to test T-lymphocyte subsets

the killing function of lymphocytes

and the contents of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2). The outcomes were compared between groups. The TIR in the ricin-gel group was 61.8%

with the pathologic examination showing extensive tumor tissue necrosis. Compared with the model group

after ricin temperature response gel treatment

bcl-2 expression was down-regulated

bax expression was up-regulated

CD4+ lymphocytes and CD4+/CD8 /CD8+ ratio in peripheral blood were increased

the killing function of lymphocytes was enhanced

and the contents of TNF-α and IL-2 were elevated (P<0.05 or P<0.01). Intratumor injection of ricin temperature-responsive gel showed significant antitumor effect on breast cancer and could enhance the immune function in the tumor-bearing rat.

Abstract

ricin

ricin-gel) were administered with blank gel

ricin

and ricin temperature response gel via percutaneous intratumor injection

respectively. The tumor was isolated 10 days later for the estimation of tumor inhibition rate (TIR) by weighing

pathologic examination

and detection of tumor apoptosis-associated genes bcl-2 and bax with semiquantitative RT-PCR. Also

peripheral blood was obtained to test T-lymphocyte subsets

the killing function of lymphocytes

and the contents of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2). The outcomes were compared between groups. The TIR in the ricin-gel group was 61.8%

with the pathologic examination showing extensive tumor tissue necrosis. Compared with the model group

after ricin temperature response gel treatment

bcl-2 expression was down-regulated

bax expression was up-regulated

CD4+ lymphocytes and CD4+/CD8 /CD8+ ratio in peripheral blood were increased

the killing function of lymphocytes was enhanced

关键词

ricingelBreast Cancermesenchymal chemotherapyimmune regulation

Keywords

ricingelBreast Cancermesenchymal chemotherapyimmune regulation

references

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