Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma

Peng-cheng Gu; Xin-sheng Fan; Chen-xue Jiang; Hui-qin Xu; Jing-hua Yu; Yu-ping Tang

doi:10.1007/s11655-011-0788-x

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Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma

OriginalPaper | Updated：2021-08-27

- Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma
- Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma
- 中国结合医学杂志（英文版） 2011年17卷第7期页码：537-541
- Affiliations：
  
  1. Department of Pharmacology, Nanjing University of Chinese Medicine,Nanjing,China
  2. Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine,Nanjing,China
  3. Clinical Trials Center, Nanjing University of Chinese Medicine,Nanjing,China
- Author bio：
- Funds：
  
  Supported by a Grant-in-aid for Scientific Research in the 11th Five-Year Plan, the Ministry of Science and Technology of P. R. China (No. 2006BAI06A02-01) and Natural Science Foundation of China (No. 81072748)
- DOI：10.1007/s11655-011-0788-x
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-7-3，
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Gu, Pc., Fan, Xs., Jiang, Cx. et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma., Chin. J. Integr. Med. 17, 537–541 (2011). https://doi.org/10.1007/s11655-011-0788-x

Peng-cheng Gu, Xin-sheng Fan, Chen-xue Jiang, et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma[J]. Chinese Journal of Integrative Medicine, 2011,17(7):537-541.
Gu, Pc., Fan, Xs., Jiang, Cx. et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma., Chin. J. Integr. Med. 17, 537–541 (2011). https://doi.org/10.1007/s11655-011-0788-x DOI：

Peng-cheng Gu, Xin-sheng Fan, Chen-xue Jiang, et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma[J]. Chinese Journal of Integrative Medicine, 2011,17(7):537-541. DOI： 10.1007/s11655-011-0788-x.

摘要

San’ao Decoction (三拗汤

SAD)

as a representative Chinese medicine (CM) formula

was chosen to evaluate the effect of airway inflammation and hyperresponsiveness on the lipopolysaccharide (LPS) enhanced asthma model. The asthma model was reproduced in the Balb/C mice sensitized by ovalbumin (OVA)

challenged by OVA and LPS. After Balb/C mice’s administration of a dose (0.0024 g/kg) of dexamethasone acetate

and three doses (2.2 g/kg

4.4 g/kg and 8.8 g/kg) of SAD

airway inflammation and responsiveness were observed. The airway inflammation was detected by counting bronchoalveolar lavage fluid (BALF) cells and lung histopathology. Also

differential expressions of interferon-r (IFN-γ)

interleukin-4 (IL-4)

and IL-5 in the supernatants of BALF were examined. The changes in airway responsiveness indicated by lung resistance (RL) and stimulated by acetylcholine (Ach) were determined. Small-dose SAD hardly inhibit airway inflammation or hyperresponsiveness in the LPS-enhanced asthma

while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness

and to some extent

reduced airway inflammation. Meanwhile

the small-dose

medium-dose

and high-dose SAD promoted Th1-type cytokines (IFN-γ) and reduced Th2-type cytokines (IL-4

IL-5) to different extents

which led to a Th1/Th2 balance. SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model

but its definite influence on airway inflammation is not remarkable.

Abstract

San’ao Decoction (三拗汤

SAD)

as a representative Chinese medicine (CM) formula

challenged by OVA and LPS. After Balb/C mice’s administration of a dose (0.0024 g/kg) of dexamethasone acetate

and three doses (2.2 g/kg

4.4 g/kg and 8.8 g/kg) of SAD

airway inflammation and responsiveness were observed. The airway inflammation was detected by counting bronchoalveolar lavage fluid (BALF) cells and lung histopathology. Also

differential expressions of interferon-r (IFN-γ)

interleukin-4 (IL-4)

while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness

and to some extent

reduced airway inflammation. Meanwhile

the small-dose

medium-dose

and high-dose SAD promoted Th1-type cytokines (IFN-γ) and reduced Th2-type cytokines (IL-4

IL-5) to different extents

which led to a Th1/Th2 balance. SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model

but its definite influence on airway inflammation is not remarkable.

关键词

San’ao DecoctionlipopolysaccharideAsthma

Keywords

San’ao DecoctionlipopolysaccharideAsthma

references

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