Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion

Guang Yang; Su-yang Cui; Li-bing Huang

doi:10.1007/s11655-011-0789-9

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Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion

OriginalPaper | Updated：2021-08-27

- Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion
- Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion
- 中国结合医学杂志（英文版） 2011年17卷第7期页码：542-545
- Affiliations：
  
  Department of Anesthesiology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-011-0789-9
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-7-3，
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Yang, G., Cui, Sy. & Huang, Lb. Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion., Chin. J. Integr. Med. 17, 542–545 (2011). https://doi.org/10.1007/s11655-011-0789-9

Guang Yang, Su-yang Cui, Li-bing Huang. Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion[J]. Chinese Journal of Integrative Medicine, 2011,17(7):542-545.
Yang, G., Cui, Sy. & Huang, Lb. Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion., Chin. J. Integr. Med. 17, 542–545 (2011). https://doi.org/10.1007/s11655-011-0789-9 DOI：

Guang Yang, Su-yang Cui, Li-bing Huang. Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion[J]. Chinese Journal of Integrative Medicine, 2011,17(7):542-545. DOI： 10.1007/s11655-011-0789-9.

摘要

To evaluate the protective effects of sodium aescinate (SA) preconditioning on the tourniquet-induced ischemia-reperfusion (I/R) injury after limbs operation. Seventy-five patients with grade I–II issued by American Society of Anesthesiology undergoing lower limb operation were randomly assigned to 3 groups: the control group

low-dose SA-treated group and high-dose SA-treated group; each group enrolled 25 patients. The patients were treated with 5 mg and 10 mg SA 30 min before tourniquet inflation in the two treatment groups separately

while the patients in the control group received normal saline. Venous blood samples were obtained before tourniquet was inflated (T0 baseline). And 5 (T1)

10 (T2)

20 (T3) min after tourniquet was released. The nitric oxide (NO)

malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined by commercial kits. Meanwhile

arterial pressure (MAP) and heart rate (HR) were monitored from an automatic invigilator. In the control group

MDA and NO levels were increased

and SOD and MAP were decreased significantly after tourniquet deflation compared to T0 baseline (P<0.05). After tourniquet deflation

MDA and NO levels in the two treated groups were significantly decreased; meanwhile

SOD levels and MAP were increased

and the variations of HR were more stable compared with the control group (all P<0.05). There was no significant difference in all of the above between the two treated groups (P>0.05). The protective effects of SA preconditioning on tourniquet-induced limb I/R injury might possibly contribute to the increasing of SOD levels

and MAP and the decreasing of MDA and NO levels.

Abstract

while the patients in the control group received normal saline. Venous blood samples were obtained before tourniquet was inflated (T0 baseline). And 5 (T1)

10 (T2)

20 (T3) min after tourniquet was released. The nitric oxide (NO)

malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined by commercial kits. Meanwhile

arterial pressure (MAP) and heart rate (HR) were monitored from an automatic invigilator. In the control group

MDA and NO levels were increased

and SOD and MAP were decreased significantly after tourniquet deflation compared to T0 baseline (P<0.05). After tourniquet deflation

MDA and NO levels in the two treated groups were significantly decreased; meanwhile

SOD levels and MAP were increased

and MAP and the decreasing of MDA and NO levels.

关键词

ischemic reperfusion injurysodium aescinatelimb

Keywords

ischemic reperfusion injurysodium aescinatelimb

references

lwata A, Morgan SV, Schwartz B, Liu L, Tupper J, Zhu X, et al. Extracellular Bcl-2 proteins are danger-associated molecular patterns that reduce tissue damage in murine models of ischemia-reperfusion injury. PLoS One 2010;5:e9103.

Kim JG, Lee J, Roe J. Hemodynamic changes in rat leg muscles during tourniquet-induced ischemia-reperfusion injury observed by near-infrared spectroscopy. Physiol Meas 2009;30:529–540.

Kato N, Abe S, Suto M, Hiraiwa K. Comparison of renal dysfunction in wild-type, IL-6 KO and iNOS KO mice hind limb tourniquet-reperfusion model. Leg Med (Tokyo) 2009;11Suppl 1:S248–251.

Sirtori CR. Aescin: pharmacology, pharmacokinetics and therapeutic profile. Pharmacol Res 2001;44:183–193.

Yang G, Cui SY. Protective effects of preconditioning with sodium aescinate on ischemia/reperfusion injury of the limbs induced by tourniquet. J Clin Rehabilitative Tissue Eng Res (Chin) 2008;12:903–906.

Hu XM, Zeng FD. Protective effects of sodium beta-aescin on ischemia-reperfusion injury in rat brain. Acta Pharm Sin (Chin) 2004;39:419–423.

Li XS, Zheng WY, Lou SX, Lu XW, Ye SH. Effect of Ginkgo leaf extract on vascular endothelial function in patients with early stage diabetic Nephropathy. Chin J Integr Med 2009;15:26–29.

Glantzounis GK, Sheth H, Thompson C, Hafez TS, Kanoria S, Pamecha V, et al. Acute limb ischemia caused by femoral arterial line induces remote liver injury in a rabbit model of liver ischemia/reperfusion injury. Angiology 2009;60:554–561.

Montenegro L, Carbone C, Giannone I, Puglisi G. Use of solid phase extraction (SPE) to evaluate in vitro skin permeation of aescin. Pharmazie 2007;62:342–345.

Carrasco OF, Vidrio H. Endothelium protectant and contractile effects of the antivaricose principle escin in rat aorta. Vascul Pharmacol 2007;47:68–73.

Patlolla JM, Raju J, Swamy MV, Rao CV. Beta-escin inhibits colonic aberrant crypt foci formation in rats and regulates the cell cycle growth by inducing p21(waf1/cip1) in colon cancer cells. Mol Cancer Ther 2006;5:1459–1466.

Turut H, Kurutas EB, Bulbuloglu E, Yasim A, Ozkaya M, Onder A, et al. Zinc aspartate alleviates lung injury induced by intestinal ischemia-reperfusion in rats. J Surg Res 2009;151:62–67.

Bhaskar L, Ramakrishna BS. Colonic mucosal antioxidant enzymes and lipid peroxide levels in normal subjects and patients with ulcerative colitis. J Gastroenterol Hepatol 1995;10:140–143.

Fu F, Hou Y, Jiang W, Wang R, Liu K. Escin: inhibiting inflammation and promoting gastrointestinal transit to attenuate formation of postoperative adhesions. World J Surg 2005;29:1614–1620.

Montopoli M, Froldi G, Comelli MC, Prosdocimi M, Caparrotta L. Aescin protection of human vascular endothelial cells exposed to cobalt chloride mimicked hypoxia and inflammatory stimuli. Planta Med 2007;73:285–288.

Lu P, Liu F, Wang CY, Chen DD, Yao Z, Tian Y, et al. Gender difference sinhepatic ischemic reperfusion injury in rats are associated with endothelial cell nitric oxide synthase-derived nitric oxide. World J Gastroenterol 2005;11:3441–3445.

Xie YC, Tang F. Protective effect of Pogostemon cablin on membrane fluidity of intestinal epithelia cell in ischemia/reperfusion rats after ischemia/reperfusion. Chin J Integr Tradit West Med (Chin) 2009;29:639–641.

Cowled PA, Khanna A, Laws PE, Field JB, Fitridge RA. Simvastatin plus nitric oxide synthase inhibition modulates remote organ damage following skeletal muscle ischemiareperfusion injury. J Invest Surg 2008;21:119–126.

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