Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids

Xiao-yu Liu; Qi Wang; Shi-jin Xia; Jian-hua Huang; Zi-yin Shen; Hao Xu

doi:10.1007/s11655-011-0848-2

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Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids

OriginalPaper | Updated：2021-08-27

- Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids
- Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids
- 中国结合医学杂志（英文版） 2011年17卷第9期页码：704-709
- Affiliations：
  
  1. Shanghai Traditional Chinese Medicine Clinical Center of Cardio-cerebrovascular Diseases in Tenth People’s Hospital of Tongji University,Shanghai,China
  2. Shanghai Biochip Co., Ltd.,Shanghai,China
  3. Huadong Hospital Affiliated to Fudan University,Shanghai,China
  4. Institute of Integrative Medicine of Fudan University,Shanghai,China
  5. National Integrative Medicine Center for Cardiovascular Diseases, China-Japan Friendship Hospital,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30500681, 30973845), Major State Basic Research Development Program of China (No. 2007CB507406), and Traditional Chinese Medicine Modernization Programs of Shanghai Science and Technology Committee (No. 09dZ1975000)
- DOI：10.1007/s11655-011-0848-2
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-9-11，
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Liu, Xy., Wang, Q., Xia, Sj. et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids., Chin. J. Integr. Med. 17, 704 (2011). https://doi.org/10.1007/s11655-011-0848-2

Xiao-yu Liu, Qi Wang, Shi-jin Xia, et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids[J]. Chinese Journal of Integrative Medicine, 2011,17(9):704-709.
Liu, Xy., Wang, Q., Xia, Sj. et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids., Chin. J. Integr. Med. 17, 704 (2011). https://doi.org/10.1007/s11655-011-0848-2 DOI：

Xiao-yu Liu, Qi Wang, Shi-jin Xia, et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids[J]. Chinese Journal of Integrative Medicine, 2011,17(9):704-709. DOI： 10.1007/s11655-011-0848-2.

摘要

To study the characteristics of lymphocyte nuclear factor kappa B (NF-κB) signal transduction kinase-related molecular mRNA differential expressions at various month age segments in aging process and the intervening effect of Epimedium flavonoids (EF) on it. Sixty SD rats were divided into six groups

according to animals’ age

i.e.

the 3 days (d) group

the 4 months (m) group

the 10 m group

the 18 m group

the 27 m group

and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes)

the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined

and the intervening effect of EF was examined. The mean level of the NF-κB signal transduction kinase-related mRNA expressions in rats’ splenic lymphocytes lowered with aging; the highest expression was presented at 3 d after birth

and then

it lowered gradually

with the lowest level at 18 m or 27 m. After EF intervention

the expression level was raised to the 10–18 m level in the aged rats. The changing rules of lymphocyte NF-κB-signal-transduction-kinase-related mRNA expressions in various stages of aging are helpful for selecting the well time for preventing and intervening aging

and will also give a hint to the molecular index for assessment of senility retarding researches.

Abstract

according to animals’ age

i.e.

the 3 days (d) group

the 4 months (m) group

the 10 m group

the 18 m group

the 27 m group

and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes)

the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined

and then

it lowered gradually

with the lowest level at 18 m or 27 m. After EF intervention

and will also give a hint to the molecular index for assessment of senility retarding researches.

关键词

nuclear factor-κB signal transduction kinasegene-chiplymphocyteagingimmunosenescenceEpimedium flavonoidsmRNA

Keywords

nuclear factor-κB signal transduction kinasegene-chiplymphocyteagingimmunosenescenceEpimedium flavonoidsmRNA

references

Peter C. Nuclear factor kappaB-molecular biomedicine: the next generation. Biomed Pharmacother 2004;58:365–371.

Lewis KN, Mele J, Hayes JD, Buffenstein R. Nrf2, a 2. guardian of healthspan and gatekeeper of species longevity. Integr Comp Biol 2010;50:829–843.

Charles G, Andrea KH. Growing old with nuclear factor-κB. Cell Stress Chaperones 2002;7:207–212.

Kriete A, Mayo KL. Atypical pathways of NF-kappaB activation and aging. Exp Gerontol 2009;44:250–255.

Häcker H, Karin M. Regulation and function of IKK and IKK-related kinases. Sci Signal 2006:357.

Bondy SC, Li H, Zhou J, Wu M, Bailey JA, Lahiri DK. Melatonin alters age-related changes in transcription factors and kinase activation. Neurochem Res 2010;35:2035–2042.

Li X, Massa PE, Hanidu A, Peet GW, Aro P, Savitt A, et al. IKKalpha, IKKbeta, and NEMO/IKKgamma are each required for the NF-kappa B-mediated inflammatory response program. J Biol Chem 2002;277:45129–45140.

Kim MK, Chung SW, Kim DH, Kim JM, Lee EK, Kim JY, et al. Modulation of age-related NF-kappaB activation by dietary zingerone via MAPK pathway. Exp Gerontol 2010;45:419–426.

Helen S, Nicolas B, Lixin Z, Alan C, Keiko S, Janet D, et al. Requirement for caspase-8 in NF-κB activation by antigen receptor. Science 2005;307:1465–1468.

Liu XY, Shen ZY, Huang JH, Chen WH. The regulatory mechanism of immunosenescence of Epimedii flavonoids through nuclear factor-κB signaling transduction pathways. Chin J Integr Med 2006; 26:620–624.

Liu XY, Shen ZY, Wu B, Xia SJ, Huang JH, Chen WH. Expression characteristics of p65, IκBα, IκBɛ in lymphocytes phosphorylation of aged rats and interventional research of Epimedii flavonoids. China J Chin Mater Med (Chin) 2008;24:73–75.

Liu XY, Shen ZY, Wang Q, Chen WH. The regulatory 12. effects of Epimedii flavonoids to Th1, Th2 and Th3 cells of aged rats. Chin J Immunol (Chin) 2005;21(11):46–49.

Shen ZY, Yuan CY, Huang JH, Guo AK, Hu ZW. The 13. molecular mechanism of drosophila life extension induced by total flavonoids from herba Epimedii. Chin J Gerontol (Chin) 2005. 25:1061–1063.

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