Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells

Yue-rong Jiang; Yu Miao; Lin Yang; Mei Xue; Chun-yu Guo; Xiao-juan Ma; Hui-jun Yin; Da-zhuo Shi; Ke-ji Chen

doi:10.1007/s11655-011-0849-1

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Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells

OriginalPaper | Updated：2021-08-27

- Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells
- Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells
- 中国结合医学杂志（英文版） 2012年18卷第1期页码：30-33
- Affiliations：
  
  Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Developing Plan for Basic Key Items (973 Plan, No. 2006CB504803)
- DOI：10.1007/s11655-011-0849-1
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2011-10-12，
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Jiang, Yr., Miao, Y., Yang, L. et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells., Chin. J. Integr. Med. 18, 30–33 (2012). https://doi.org/10.1007/s11655-011-0849-1

Yue-rong Jiang, Yu Miao, Lin Yang, et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells[J]. Chinese Journal of Integrative Medicine, 2012,18(1):30-33.
Jiang, Yr., Miao, Y., Yang, L. et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells., Chin. J. Integr. Med. 18, 30–33 (2012). https://doi.org/10.1007/s11655-011-0849-1 DOI：

Yue-rong Jiang, Yu Miao, Lin Yang, et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells[J]. Chinese Journal of Integrative Medicine, 2012,18(1):30-33. DOI： 10.1007/s11655-011-0849-1.

摘要

To investigate the effects of drug-containing serum of Chinese herbal compound

Xiongshao Capsule (芎芍胶囊

for activating-blood) and Huanglian Capsule (黄连胶囊

for dispellingtoxin) on the oxidized low-density lipoprotein (ox-LDL)-induced inflammatory factors in human umbilical vein endothelial cells (HUVECs). Thirty-two rats were randomly divided into four groups: the blank control group treated with distilled water

the positive control group treated with simvastatin (1.8 mg/kg)

the test group I treated with Chinese herbal compound of XS (0.135 g/kg)

and the test group II treated with Chinese herbal compound of XS (0.135 g/kg) and HL (0.135 g/kg). All the treatments were administered for 7 successive days by gastrogavage. Rats’ blood serum was harvested 1 h after the last administration to prepare respective drugcontaining serum. HUVECs were exposed to ox-LDL (100 μg/mL) to induce cell injury model and incubated with corresponding drug-containing serum for 24 h. Untreated HUVECs were set for blank control. Levels of interleukin-6 (IL-6)

tumor necrosis factor-α (TNF-α)

and soluble intercellular adhesion molecule-1 (sICAM-1) in supernatant of cultured HUVECs were determined by enzyme-linked immunosorbent assay (ELISA). HUVEC surface expressions of ICAM-1 and E-selectin were determined by flow cytometry. Levels of IL-6

TNF-α

and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation (P<0.01)

while the abnormal elevations

except sICAM-1 in the test group I

were all reduced in the treated groups (the positive control and the two test groups) significantly (P<0.01 or P<0.05). Besides

the effect in the test group II seemed somewhat higher than that in the test group I but with no statistical significance (P>0.05). Drug-containing serum of XS plus HL has a certain inhibitory effect on the vascular endothelial inflammation response induced by ox-LDL.

Abstract

To investigate the effects of drug-containing serum of Chinese herbal compound

Xiongshao Capsule (芎芍胶囊

for activating-blood) and Huanglian Capsule (黄连胶囊

the positive control group treated with simvastatin (1.8 mg/kg)

the test group I treated with Chinese herbal compound of XS (0.135 g/kg)

tumor necrosis factor-α (TNF-α)

TNF-α

and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation (P<0.01)

while the abnormal elevations

except sICAM-1 in the test group I

were all reduced in the treated groups (the positive control and the two test groups) significantly (P<0.01 or P<0.05). Besides

关键词

activating blood and dispelling toxindrug-containing serumendothelial celloxidized low-density lipoproteininflammation

Keywords

activating blood and dispelling toxindrug-containing serumendothelial celloxidized low-density lipoproteininflammation

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