Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer

Xue-lin Wang; Feng Ma; Xiong-zhi Wu

doi:10.1007/s11655-011-0878-9

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Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer

OriginalPaper | Updated：2021-08-27

- Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer
- Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer
- 中国结合医学杂志（英文版） 2012年18卷第8期页码：599-604
- Affiliations：
  
  Zhong-Shan-Men In-patient Department, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy,Tianjin,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30801495)
- DOI：10.1007/s11655-011-0878-9
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2011-12-8，
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Wang, Xl., Ma, F. & Wu, Xz. Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer., Chin. J. Integr. Med. 18, 599–604 (2012). https://doi.org/10.1007/s11655-011-0878-9

Xue-lin Wang, Feng Ma, Xiong-zhi Wu. Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer[J]. Chinese Journal of Integrative Medicine, 2012,18(8):599-604.
Wang, Xl., Ma, F. & Wu, Xz. Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer., Chin. J. Integr. Med. 18, 599–604 (2012). https://doi.org/10.1007/s11655-011-0878-9 DOI：

Xue-lin Wang, Feng Ma, Xiong-zhi Wu. Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer[J]. Chinese Journal of Integrative Medicine, 2012,18(8):599-604. DOI： 10.1007/s11655-011-0878-9.

摘要

To investigate the anticancer effects of warming and relieving cold phlegm formula (温化寒痰复方

WRCP)

a Chinese medical mixture composed of the aqueous extracts of Aconitum carmichaeli

Rhizoma bolbostemmatis

Phytolacca acinosa

Panax notoginseng

and Gekko swinhonis Gūenther

combined with 5-fluorouracil (5-FU) on human breast cancer in vivo. Seventy-two Nu/Nu mice inoculated with MDA-MB-231 breast cancer cells were randomized into the control group

5-FU group

high-dose WRCP (hWRCP) group

medium-dose WRCP (mWRCP) group

low-dose WRCP (lWRCP) group

or combination of mWRCP and 5-FU group in a 1:1:1:1:1:1 ratio. Drug administration was commenced on the day following tumor implantation. The control group was injected daily with normal saline (N.S.) intraperitoneally; the 5-FU group was injected with 5-FU at 30 mg/kg intraperitoneally every third day for a total of 7 treatments; the hWRCP group

mWRCP group and lWRCP group received daily doses of 5

and 0.2 g/kg of WRCP

respectively

by gastric perfusion; and the combination group was treated with 5-FU plus mWRCP on the same schedules as above. All treatments lasted for 22 days. Tumor volume

tumor weight

inhibition rate of tumor weight

necrosis rate of tumor

organ index

and change in body weight of nude mice were measured. The combination group and the hWRCP group had significantly smaller tumor volumes (580±339 mm3 and 587±249 mm3 versus 1055±234 mm3

respectively)

lower tumor weights (0.42±0.29 g and 0.52±0.29 g versus 0.80±0.15 g

respectively)

and higher tumor necrosis rates (22.7% and 25.6% versus 9.4%

respectively) as compared with the control group (all <0.05). Similar changes were found in the 5-FU

mWRCP

and lWRCP groups when compared with the control group but were not statistically significant

except for the tumor weight for the 5-FU group. The combination group and the hWRCP group had significantly smaller tumor volumes compared with the 5-FU group (778±202 mm3

both <0.05). The combination group had the highest tumor inhibition rate (47.7%)

followed by the hWRCP group (35.2%) and 5-FU group (28.3%). The 5-FU group had a lower body weight increase (1.37±2.06 g versus 5.60±0.72 g

<0.05) and a lower spleen index (4.064±1.774 mg/10 g versus 5.294±1.796 mg/10 g) as compared with the control group

whereas the combination group reversed the changes in the 5-FU group with the body weight increase of 3.52±1.80 g (P <0.05) and spleen index of 7.036±1.599 mg/10 g (P <0.05). The spleen indices in the hWRCP

mWRCP

and IWRCP group were all significantly higher than that in the 5-FU group (P <0.01 or P<0.05). No significant differences in body weight change were observed in WRCP groups compared with the control group P>0.05). The treatment combination of WRCP and 5-FU was more effective in the inhibition of tumor growth than either agent alone and may have potentially additional benefit in improving the general condition and immunity of the mice with human breast cancer cell implants.

Abstract

To investigate the anticancer effects of warming and relieving cold phlegm formula (温化寒痰复方

WRCP)

a Chinese medical mixture composed of the aqueous extracts of Aconitum carmichaeli

Rhizoma bolbostemmatis

Phytolacca acinosa

Panax notoginseng

and Gekko swinhonis Gūenther

combined with 5-fluorouracil (5-FU) on human breast cancer in vivo. Seventy-two Nu/Nu mice inoculated with MDA-MB-231 breast cancer cells were randomized into the control group

5-FU group

high-dose WRCP (hWRCP) group

medium-dose WRCP (mWRCP) group

low-dose WRCP (lWRCP) group

mWRCP group and lWRCP group received daily doses of 5

and 0.2 g/kg of WRCP

respectively

by gastric perfusion; and the combination group was treated with 5-FU plus mWRCP on the same schedules as above. All treatments lasted for 22 days. Tumor volume

tumor weight

inhibition rate of tumor weight

necrosis rate of tumor

organ index

and change in body weight of nude mice were measured. The combination group and the hWRCP group had significantly smaller tumor volumes (580±339 mm3 and 587±249 mm3 versus 1055±234 mm3

respectively)

lower tumor weights (0.42±0.29 g and 0.52±0.29 g versus 0.80±0.15 g

respectively)

and higher tumor necrosis rates (22.7% and 25.6% versus 9.4%

respectively) as compared with the control group (all <0.05). Similar changes were found in the 5-FU

mWRCP

and lWRCP groups when compared with the control group but were not statistically significant

except for the tumor weight for the 5-FU group. The combination group and the hWRCP group had significantly smaller tumor volumes compared with the 5-FU group (778±202 mm3

both <0.05). The combination group had the highest tumor inhibition rate (47.7%)

followed by the hWRCP group (35.2%) and 5-FU group (28.3%). The 5-FU group had a lower body weight increase (1.37±2.06 g versus 5.60±0.72 g

<0.05) and a lower spleen index (4.064±1.774 mg/10 g versus 5.294±1.796 mg/10 g) as compared with the control group

mWRCP

关键词

Breast Cancerwarming and relieving cold phlegm formula5-fluorouracilangiogenesis

Keywords

Breast Cancerwarming and relieving cold phlegm formula5-fluorouracilangiogenesis

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