Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease

Xi Li; Hai-feng Yuan; Qian-kun Quan; Jian-jun Wang; Ning-ning Wang; Ming Li

doi:10.1007/s11655-011-0896-7

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Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease

OriginalPaper | Updated：2021-08-27

- Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease
- Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease
- 中国结合医学杂志（英文版） 2011年17卷第11期页码：847-853
- Affiliations：
  
  1. Department of Geriatrics, the Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University,Xi’an,China
  2. Department of Neurology, the Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University,Xi’an,China
  3. Research Center of Rehabilitation Science and Technology, School of Life Science and Technology, Xi’an Jiaotong University,Xi’an,China
  4. Department of Geriatrics, Fuzhou General Hospital of Nanjing Military District,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by Tackle Key Problems in-Science and Technology Foundation of Shaanxi Province [No.2007K16-07(5)]
- DOI：10.1007/s11655-011-0896-7
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-11-6，
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Li, X., Yuan, Hf., Quan, Qk. et al. Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease., Chin. J. Integr. Med. 17, 847–853 (2011). https://doi.org/10.1007/s11655-011-0896-7

Xi Li, Hai-feng Yuan, Qian-kun Quan, et al. Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease[J]. Chinese Journal of Integrative Medicine, 2011,17(11):847-853.
Li, X., Yuan, Hf., Quan, Qk. et al. Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease., Chin. J. Integr. Med. 17, 847–853 (2011). https://doi.org/10.1007/s11655-011-0896-7 DOI：

Xi Li, Hai-feng Yuan, Qian-kun Quan, et al. Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease[J]. Chinese Journal of Integrative Medicine, 2011,17(11):847-853. DOI： 10.1007/s11655-011-0896-7.

摘要

To observe the effect of a Chinese medicine compound

Naoerkang (脑尔康

NEK)

on amyloid-beta peptide (1–42; Aβ1–42) and matrix metalloproteinase-9 (MMP-9) expressions in the hippocampus of Alzheimer’s disease (AD) model rats. A total of 48 male Sprague Dawley (SD) rats were randomly divided into normal control

untreated

and piracetam groups

and low-dose

medium-dose

and high-dose NEK groups

with 8 rats in each group. The 5-μL aggregated Aβ1–42 (2 μg/μL) were injected into both CA1 areas of the hippocampus in the rats to establish an AD model

whereas the normal control was treated with the same dose of normal saline. The rats in the NEK groups were treated with a high

medium

or low dose of NEK [60 g/(kg·d)

30 g/(kg·d)

and 15 g/(kg·d)]

respectively

intragastrically for 28 days; piracetam (0.375 g/kg

intragastrically) was consecutively administered in the piracetam group; and normal saline was applied in the normal control and untreated groups. A Y-maze test was used for behavioral study to test the learning and memory abilities. Aβ1–42 and MMP-9 expressions in the hippocampus was determined immunohistochemically

and the results were analyzed by image acquisition and an analysis system. Aggregated Aβ1–42 induced obvious learning and memory dysfunction

as well as up-regulation of Aβ1–42 expression in the hippocampus. Compared with those in the normal control group

the learning and memory abilities of rats in the untreated group significantly decreased (P<0.01)

and the expression of Aβ1–42 was significantly increased (P<0.01). Twenty-eight days after different treatments

compared with those in the untreated group

the learning and memory abilities of AD model rats in the piracetam

low-dose

medium-dose and high-dose NEK groups were significantly improved (P<0.01 or P<0.05)

and the expression of Aβ1–42 in the hippocampus decreased (P<0.01 or P<0.05)

and MMP-9 increased (P<0.01 or P<0.05)

especially in the high-dose NEK group. NEK might play a role of anti-dementia by increasing the expression of MMP-9 in the hippocampus of AD model rats

resulting in the reduction of the quantity of Aβ1–42 and improvement in learning and memory ability in AD model rats.

Abstract

To observe the effect of a Chinese medicine compound

Naoerkang (脑尔康

NEK)

untreated

and piracetam groups

and low-dose

medium-dose

and high-dose NEK groups

with 8 rats in each group. The 5-μL aggregated Aβ1–42 (2 μg/μL) were injected into both CA1 areas of the hippocampus in the rats to establish an AD model

whereas the normal control was treated with the same dose of normal saline. The rats in the NEK groups were treated with a high

medium

or low dose of NEK [60 g/(kg·d)

30 g/(kg·d)

and 15 g/(kg·d)]

respectively

intragastrically for 28 days; piracetam (0.375 g/kg

and the results were analyzed by image acquisition and an analysis system. Aggregated Aβ1–42 induced obvious learning and memory dysfunction

as well as up-regulation of Aβ1–42 expression in the hippocampus. Compared with those in the normal control group

the learning and memory abilities of rats in the untreated group significantly decreased (P<0.01)

and the expression of Aβ1–42 was significantly increased (P<0.01). Twenty-eight days after different treatments

compared with those in the untreated group

the learning and memory abilities of AD model rats in the piracetam

low-dose

medium-dose and high-dose NEK groups were significantly improved (P<0.01 or P<0.05)

and the expression of Aβ1–42 in the hippocampus decreased (P<0.01 or P<0.05)

and MMP-9 increased (P<0.01 or P<0.05)

especially in the high-dose NEK group. NEK might play a role of anti-dementia by increasing the expression of MMP-9 in the hippocampus of AD model rats

resulting in the reduction of the quantity of Aβ1–42 and improvement in learning and memory ability in AD model rats.

关键词

NaoerkangAlzheimer’s diseaseamyloid beta-peptidematrix metalloproteinase-9rats

Keywords

NaoerkangAlzheimer’s diseaseamyloid beta-peptidematrix metalloproteinase-9rats

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