Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism

Ying-dong Li; Yan-hua Ma; Jian-xiong Zhao; Xin-ke Zhao

doi:10.1007/s11655-011-0897-6

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Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism

OriginalPaper | Updated：2021-08-27

- Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism
- Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism
- 中国结合医学杂志（英文版） 2011年17卷第11期页码：854-859
- Affiliations：
  
  1. Gansu College of Traditional Chinese Medicine,Lanzhou,China
  2. Graduate Institute of Integration Chinese and Western Medicine, Lanzhou University,Lanzhou,China
- Author bio：
- Funds：
  
  Supported by the Key Projects of the National Science and Technology Pillar Program during the Eleventh Five-Year Plan Period (No. 2007BAI37B01) and Major Science and Technology Program of Gansu Province (No. 092NKDA017)
- DOI：10.1007/s11655-011-0897-6
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-11-6，
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Li, Yd., Ma, Yh., Zhao, Jx. et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism., Chin. J. Integr. Med. 17, 854–859 (2011). https://doi.org/10.1007/s11655-011-0897-6

Ying-dong Li, Yan-hua Ma, Jian-xiong Zhao, et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism[J]. Chinese Journal of Integrative Medicine, 2011,17(11):854-859.
Li, Yd., Ma, Yh., Zhao, Jx. et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism., Chin. J. Integr. Med. 17, 854–859 (2011). https://doi.org/10.1007/s11655-011-0897-6 DOI：

Ying-dong Li, Yan-hua Ma, Jian-xiong Zhao, et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism[J]. Chinese Journal of Integrative Medicine, 2011,17(11):854-859. DOI： 10.1007/s11655-011-0897-6.

摘要

To investigate whether the administration of the ultra-filtration extract from Danggui Buxue Decoction (当归补血汤

EDBD) was able to protect cardiomyocytes from oxidative injury of rats induced by hydrogen peroxide (H2O2) and its potential mechanism. Myocardial cells from 1- to 2-day-old neonatal rats were cultured in Dulbecco’s modified Eagle’s medium low-glucose and Ham’s F12 medium (1:1)

and the cellular injury was induced by H2O2. The ultra-filtration extract mixture from Angelica sinensis and Hedysarum polybotrys was given in three doses of 3.75

7.5

and 15 mg/mL. Morphological changes of cardiomyocytes were observed by microscope. Survival rate of myocardial cells was assessed using 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. The cardiomyocyte damages were estimated by detecting lactate dehydrogenase (LDH) and creatine kinase (CK) releases in the medium

superoxide dismutase (SOD) activities

and intracellular malondialdehyde (MDA) and myeloperoxidase (MPO) contents. The levels of caspase-3 and heat shock protein 70 (hsp70) mRNA expression in cardiomyocytes were measured by reverse transcription polymerase chain reaction. The EDBD could protect the cardiomyocytes from H2O2 injury in a dosedependent manner (3.75

7.50

and 15.00 mg/mL). The EDBD could significantly decrease LDH and CK leakages and intracellular MDA and MPO contents

increase SOD activity

up-regulate hsp70 expression

and down-regulate caspase-3 expression. The EDBD has protection on cardiomyocytes injured by H2O2 through improving cell antioxidant ability

up-regulating hsp70 expression

and inhibiting caspase-3 activity.

Abstract

To investigate whether the administration of the ultra-filtration extract from Danggui Buxue Decoction (当归补血汤

and the cellular injury was induced by H2O2. The ultra-filtration extract mixture from Angelica sinensis and Hedysarum polybotrys was given in three doses of 3.75

7.5

and 15 mg/mL. Morphological changes of cardiomyocytes were observed by microscope. Survival rate of myocardial cells was assessed using 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. The cardiomyocyte damages were estimated by detecting lactate dehydrogenase (LDH) and creatine kinase (CK) releases in the medium

superoxide dismutase (SOD) activities

7.50

and 15.00 mg/mL). The EDBD could significantly decrease LDH and CK leakages and intracellular MDA and MPO contents

increase SOD activity

up-regulate hsp70 expression

and down-regulate caspase-3 expression. The EDBD has protection on cardiomyocytes injured by H2O2 through improving cell antioxidant ability

up-regulating hsp70 expression

and inhibiting caspase-3 activity.

关键词

ultra-filtration extract from Danggui Buxue Decoction (当归补血汤)oxidative injurycardiomyocyteheat shock protein 70

Keywords

ultra-filtration extract from Danggui Buxue Decoction (当归补血汤)oxidative injurycardiomyocyteheat shock protein 70

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