Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime

Xue-zhang Zhou; Fang Jia; Xiao-ming Liu; Cong Yang; Li Zhao; Yu-jiong Wang

doi:10.1007/s11655-011-0899-4

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Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime

OriginalPaper | Updated：2021-08-27

- Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime
- Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime
- 中国结合医学杂志（英文版） 2013年19卷第12期页码：945-952
- Affiliations：
  
  Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, College of Life Science, Ningxia University,Yinchuan,China
- Author bio：
- Funds：
  
  Supported by a Sub-project of National Basic Research Program of China (No. 2006CB504401), the National Natural Science Foundation of China (No. 31060348, No. 30860207), Grants of Science and Technology Program of Ningxia to Yujiong Wang (No. Z2006-1-75001, KGZ-12-10-02)
- DOI：10.1007/s11655-011-0899-4
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2012-4-11，
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Zhou, Xz., Jia, F., Liu, Xm. et al. Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime., Chin. J. Integr. Med. 19, 945–952 (2013). https://doi.org/10.1007/s11655-011-0899-4

Xue-zhang Zhou, Fang Jia, Xiao-ming Liu, et al. Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime[J]. Chinese Journal of Integrative Medicine, 2013,19(12):945-952.
Zhou, Xz., Jia, F., Liu, Xm. et al. Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime., Chin. J. Integr. Med. 19, 945–952 (2013). https://doi.org/10.1007/s11655-011-0899-4 DOI：

Xue-zhang Zhou, Fang Jia, Xiao-ming Liu, et al. Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum β-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime[J]. Chinese Journal of Integrative Medicine, 2013,19(12):945-952. DOI： 10.1007/s11655-011-0899-4.

摘要

To evaluate the antimicrobial activity of total alkaloids extracted from Sophorea alopecuroides L. (TASA) against clinical isolated extended-spectrum beta-lactamases (ESBLs) producing Escherichia coli (E. coli) strains. The antibacterial activity of TASA either itself or in combination with cefotaxime (CTX) or ceftazidime (CAZ) was investigated by using the microbroth dilution method and phenotypic confirmatory disk diffusion test against three clinical isolated ESBLs-producing E. coli strains; the interactions of TASA and CTX or CAZ were ascertained by evaluating the fractional inhibitory concentration index (FICI). The antibacterial activity of either TASA itself or in combination with CTX or CAZ was found. The minimum inhibitory concentration (MICs) of TASA against the ESBLs producing isolates was 12.5 mg/mL. In the combinations with a sub-inhibitory concentration of TASA

a synergistic effect on CTX and CAZ against the ESBLs producing isolates was observed. Similarly

the isolates exposed to lower dose of TASA yielded an increased susceptibility to CTX and CAZ by 8–16 folds determined by microdilution assay. Moreover

enzymatic detection of ESBLs demonstrated that TASA induced reversal resistance to CTX and CAZ partially by a mechanism of inhibition of ESBLs activity in these isolates. Additionally

in the tested isolates following the exposure of TASA

molecular analysis verified the SHV-type beta-lactamase encoding ESBL gene in these isolates

and no mutation was introduced into the ESBL gene. These results suggest that TASA could be used as a source of natural compound with pharmacological activity of reversal resistance to antimicrobial agent. These findings also indicated that the application of the TASA in combination with antibiotics might prove useful in the control and treatment of infectious diseases caused by the ESBLs producing enterobacteriaceae.

Abstract

a synergistic effect on CTX and CAZ against the ESBLs producing isolates was observed. Similarly

the isolates exposed to lower dose of TASA yielded an increased susceptibility to CTX and CAZ by 8–16 folds determined by microdilution assay. Moreover

enzymatic detection of ESBLs demonstrated that TASA induced reversal resistance to CTX and CAZ partially by a mechanism of inhibition of ESBLs activity in these isolates. Additionally

in the tested isolates following the exposure of TASA

molecular analysis verified the SHV-type beta-lactamase encoding ESBL gene in these isolates

关键词

total alkaloidsSophorea alopecuroides L.extended spectrum β-lactamasesantibiotic resistanceEscherichia coli

Keywords

total alkaloidsSophorea alopecuroides L.extended spectrum β-lactamasesantibiotic resistanceEscherichia coli

references

Bagamboula CF, Uyttendaele M, Debevere J. Antimicrobial effect of spices and herbs on Shigella sonnei and Shigella flexneri. J Food Prot 2003;66:668–673.

Zuo GY, Wang GC, Zhao YB, Xu GL, Hao XY, Han J, et al. Screening of Chinese medicinal plants for inhibition against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). J Ethnopharmacol 2008;120:287–290.

Darwish RM, Aburjai TA. Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli. BMC Complement Altern Med 2010;10:9.

Baker DD, Alvi KA. Small-molecule natural products: new structures, new activities. Curr Opin Biotechnol 2004;15:576–583.

Baker DD, Chu M, Oza U, Rajgarhia V. The value of natural products to future pharmaceutical discovery. Nat Prod Rep 2007;24:1225–1244.

Chen L, Cheng X, Shi W, Lu Q, Go VL, Heber D, et al. Inhibition of growth of Streptococcus mutans, methicillinresistant Staphylococcus aureus, and vancomycinresistant enterococci by kurarinone, a bioactive flavonoid isolated from Sophora flavescens. J Clin Microbiol 2005;43:3574–3575.

Kuroyanagi M, Arakawa T, Hirayama Y, Hayashi T. Antibacterial and antiandrogen flavonoids from Sophora flavescens. J Nat Prod 1999;62:1595–1599.

Fakhimi A, Iranshahi M, Emami SA, Amin-Ar-Ramimeh E, Zarrini G, Shahverdi AR. Sophoraflavanone G from sophora pachycarpa enhanced the antibacterial activity of gentamycin against Staphylococcus aureus. Z Naturforsch C 2006;61:769–772.

Liu XJ, Cao MA, Li WH, Shen CS, Yan SQ, Yuan CS. Alkaloids from Sophora flavescens Aition. Fitoterapia 2010;81:524–527.

Ma XM, Bao G, Wan JM, Liao DJ, Yin Sh F, Meng XQ, et al. Therapeutic effects of Sophora moorcroftiana alkaloids in combination with albendazole in mice experimentally infected with protoscolices of Echinococcus granulosus. Braz J Med Biol Res 2007;40:1403–1408.

Zhou Y, Shan H, Qiao G, Sui X, Lu Y, Yang B. Inotropic effects and mechanisms of matrine, a main alkaloid from Sophora flavescens AIT. Biol Pharm Bull 2008;31:2057–2062.

Zhou Y, Wang H, Liang L, Zhao WC, Chen Y, Deng HZ. Total alkaloids of Sophora alopecuroides increases the expression of CD4+ CD25+ Tregs and IL-10 in rats with experimental colitis. Am J Chin Med 2010;38:265–277.

Sohn HY, Son KH, Kwon CS, Kwon GS, Kang SS. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine 2004;11:666–672.

Cha JD, Moon SE, Kim JY, Jung EK, Lee YS. Antibacterial activity of sophoraflavanone G isolated from the roots of Sophora flavescens against methicillin-resistant Staphylococcus aureus. Phytother Res 2009;23:1326–1331.

Klitgaard JK, Skov MN, Kallipolitis BH, Kolmos HJ. Reversal of methicillin resistance in Staphylococcus aureus by thioridazine. J Antimicrob Chemother 2008;62:1215–1221.

CLSI: Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute 2005; 15th informational supplement: p: M100–S115.

Kobayashi I, Muraoka H, Saika T, Nishida M, Fujioka T, Nasu M. Micro-broth dilution method with air-dried microplate for determining MICs of clarithromycin and amoxycillin for Helicobacter pylori isolates. J Med Microbiol 2004;53:403–406.

Menon T, Bindu D, Kumar CP, Nalini S, Thirunarayan MA. Comparison of double disc and three dimensional methods to screen for ESBL producers in a tertiary care hospital, Indian J Med Microbiol 2006;24:117–120.

Petersen PJ, Labthavikul P, Jones CH, Bradford PA. In vitro antibacterial activities of tigecycline in combination with other antimicrobial agents determined by chequerboard and time-kill kinetic analysis. J Antimicrob Chemother 2006; 57:573–576.

Coudron PE, Moland ES, Thomson KS. Occurrence and detection of AmpC beta-lactamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates at a veterans medical center. J Clin Microbiol 2000; 38:1791–1796.

Nasim K, Elsayed S, Pitout JD, Conly J, Church DL, Gregson DB. New method for laboratory detection of AmpC beta-lactamases in scherichia coli and Klebsiella pneumoniae. J Clin Microbiol 2004;42:4799–4802.

Jemima SA, Verghese S. SHV-28, an extended-spectrum beta-lactamase produced by a clinical isolate of Klebsiella pneumoniae in south India. Indian J Med Microbiol 2009;27:51–54.

Lorian D. Antibiotics in Laboratory Medicine. Edited by V L. New York: Lippincott Williams & Wilkins Press; 1996:503–578.

Sambrook R. Molecular cloning: a laboratory manual. Edited by Cold Spring Harbor. N.Y: Cold Spring Harbor Laboratory; 2001.

Livermore DM. Beta-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev 1995;8:557–584.

Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 2001;14:933–951.

Drawz SM, Bonomo RA. Three decades of beta-lactamase inhibitors. Clin Microbiol Rev 2010;23:160–201.

Kang SS, Kim JS, Son KH, Chang HW, Kim HP. A new prenylated flavanone from the roots of Sophora flavescens. Fitoterapia 2000; 71:511–515.

Ding PL, Hou AJ, Chen DF. Three new isoprenylated flavonoids from the roots of Sophora flavescens. J Asian Nat Prod Res 2005;7:237–243.

Atta-Ur-Rahman A, Choudhary MI, Parvez K, Ahmed A, Akhtar F, Nur EAM, et al. Quinolizidine alkaloids from Sophora alopecuroides. J Nat Prod 2000; 63:190–192.

Zhao G, Du W, Wei Y, Qin H, Wu X. An HPLC method for the determination and pharmacokinetic study of lehmannine in rat plasma. Biomed Chromatogr 2008;22:1149–1154.

Zhou Y, Wei Y, Liu H, Zhang G, Wu X. Preparation and in vitro evaluation of ethosomal total alkaloids of Sophora alopecuroides loaded by a transmembrane pH-gradient method. AAPS PharmSciTech 2010;11:1350–1358.

Mahamoud A, Chevalier J, Alibert-Franco S, Kern WV, Pages JM. Antibiotic efflux pumps in Gram-negative bacteria: the inhibitor response strategy. J Antimicrob Chemother 2007;59:1223–1229.

Pages JM, Alibert-Franco S, Mahamoud A, Bolla JM, Davin-Regli A, Chevalier J, et al. Efflux pumps of gram-negative bacteria, a new target for new molecules. Curr Top Med Chem 2010;10:1848–1857.

Cha JD, Jeong MR, Jeong SI, Lee KY. Antibacterial activity of sophoraflavanone G isolated from the roots of Sophora flavescens. J Microbiol Biotechnol 2007;17:858–864.

Coutinho HD, Costa JG, Lima EO, Falcao-Silva VS, Siqueira JP, Jr. Herbal therapy associated with antibiotic therapy: potentiation of the antibiotic activity against methicillin-resistant Staphylococcus aureus by Turnera ulmifolia L. BMC Complement Altern Med 2009;9:13.

Hemaiswarya S, Kruthiventi AK, Doble M. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine 2008;15:639–652.

Wagner H, Ulrich-Merzenich G. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine 2009;16:97–110.

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