In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer

Xu-ming Ji; Bing Ouyang; Heng Liu; Guo-wei Liu; Zhi-chun Wu; Hua-yun Yu; Chun-yan Wang; Zhong-xia Wang; Wen-ping Wang

doi:10.1007/s11655-011-0934-5

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In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer

OriginalPaper | Updated：2021-08-27

- In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer
- In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer
- 中国结合医学杂志（英文版） 2011年17卷第12期页码：908-916
- Affiliations：
  
  School of Basic Medical Science, Shandong University of Traditional Chinese Medicine,Jinan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072832;No.30873222) and Shandong Provincial Young and Middle-aged Scientists (No. 2008BS03010)
- DOI：10.1007/s11655-011-0934-5
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2011-12-3，
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Ji, Xm., Ouyang, B., Liu, H. et al. , In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer., Chin. J. Integr. Med. 17, 908–916 (2011). https://doi.org/10.1007/s11655-011-0934-5

Xu-ming Ji, Bing Ouyang, Heng Liu, et al. In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer[J]. Chinese Journal of Integrative Medicine, 2011,17(12):908-916.
Ji, Xm., Ouyang, B., Liu, H. et al. , In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer., Chin. J. Integr. Med. 17, 908–916 (2011). https://doi.org/10.1007/s11655-011-0934-5 DOI：

Xu-ming Ji, Bing Ouyang, Heng Liu, et al. In Vitro and In Vivo inhibitory effect of the combination of Wenxia Changfu Formula (温下肠腑方) with cisplatin in non-small cell lung cancer[J]. Chinese Journal of Integrative Medicine, 2011,17(12):908-916. DOI： 10.1007/s11655-011-0934-5.

摘要

To observe the effect of the combination of Wenxia Changfu Formula (温下肠腑方

WCF) with cisplatin (CDDP) on inhibiting non-small cell lung cancer (NSCLC) in vitro and In Vivo and explore its mechanism from its effect on cell cycle. In vitro

WCF-containing serum was prepared and the rhubarb b1

emodin

and aconitine were detected qualitatively by high-performance liquid chromatogram (HPLC). A549 cell lines were treated with blank control (dimethyl sulfoxide)

normal serum

normal serum with CDDP (1.25

2.5

and 5.0 μg/mL

respectively)

WCF-containing serum plus different doses of CDDP (1.25

2.5

and 5.0 μg/mL

respectively). The inhibitory effect was detected by 3-(4

5)-dimethylthiazo(-zy1)-3

5-diphenylterazolium bromide (MTT). The cell cycle was detected by flow cytometry. The protein and mRNA expressions of cyclin D1

proliferating cell nuclear antigen (PCNA)

retinoblastoma (Rb)

and p16 were observed with immunofluorescence and RT-PCR

respectively. In Vivo

nude mice xenograft model was established and grouped into the control

CDDP

WCF

and combination groups. The combination’s inhibition of tumor growth and influence on the weight

spleen

and thymus gland were observed. The inhibitory rate of the combination against A549 cell lines excelled the CDDP alone significantly (P <0.05); the combination showed a synergism inhibitory effect (Q=1.19). Compared with the monotherapy

the combination increased the cell percentage in G0/G1 phase and decreased the cell percentage in S phase significantly (P <0.05); the protein and mRNA expressions of cyclin D1

PCNA

and Rb were significantly reduced; the protein and mRNA expressions of p16 were significantly enhanced. Compared with the monotherapy

the combination inhibited the tumor growth significantly In Vivo and reduced the weight of tumor (P <0.05); compared with the CDDP group

the spleen and thymus gland index of the combination group were enhanced significantly (P <0.05). The combination of WCF with CDDP significantly inhibited the A549 cell lines proliferation in vitro and the growth of the tumor In Vivo; it inhibited effectively the atrophy of the immune organ caused by chemotherapy. The combination inhibited overproliferation of A549 cell lines by arresting the G0 /G1 phase of cell cycle and affecting the protein and mRNA expressions of cell cycle-related proteins

cyclin D1

etc.

Abstract

To observe the effect of the combination of Wenxia Changfu Formula (温下肠腑方

WCF) with cisplatin (CDDP) on inhibiting non-small cell lung cancer (NSCLC) in vitro and In Vivo and explore its mechanism from its effect on cell cycle. In vitro

WCF-containing serum was prepared and the rhubarb b1

emodin

and aconitine were detected qualitatively by high-performance liquid chromatogram (HPLC). A549 cell lines were treated with blank control (dimethyl sulfoxide)

normal serum

normal serum with CDDP (1.25

2.5

and 5.0 μg/mL

respectively)

WCF-containing serum plus different doses of CDDP (1.25

2.5

and 5.0 μg/mL

respectively). The inhibitory effect was detected by 3-(4

5)-dimethylthiazo(-zy1)-3

5-diphenylterazolium bromide (MTT). The cell cycle was detected by flow cytometry. The protein and mRNA expressions of cyclin D1

proliferating cell nuclear antigen (PCNA)

retinoblastoma (Rb)

and p16 were observed with immunofluorescence and RT-PCR

respectively. In Vivo

nude mice xenograft model was established and grouped into the control

CDDP

WCF

and combination groups. The combination’s inhibition of tumor growth and influence on the weight

spleen

the combination increased the cell percentage in G0/G1 phase and decreased the cell percentage in S phase significantly (P <0.05); the protein and mRNA expressions of cyclin D1

PCNA

and Rb were significantly reduced; the protein and mRNA expressions of p16 were significantly enhanced. Compared with the monotherapy

the combination inhibited the tumor growth significantly In Vivo and reduced the weight of tumor (P <0.05); compared with the CDDP group

cyclin D1

etc.

关键词

Chinese MedicineWenxia Changfu Formulachemotherapyinhibitory effectcell cyclecell cycle-related proteinsnon-small cell lung cancer

Keywords

Chinese MedicineWenxia Changfu Formulachemotherapyinhibitory effectcell cyclecell cycle-related proteinsnon-small cell lung cancer

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