Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit

Xiang-ying Kong; Rong-tian Wang; Neng Tian; Li Li; Na Lin; Wei-heng Chen

doi:10.1007/s11655-011-0940-7

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Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit

OriginalPaper | Updated：2021-08-27

- Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit
- Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit
- 中国结合医学杂志（英文版） 2012年18卷第10期页码：761-768
- Affiliations：
  
  1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
  2. Wangjing Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30672770)
- DOI：10.1007/s11655-011-0940-7
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-1-12，
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Kong, Xy., Wang, Rt., Tian, N. et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit., Chin. J. Integr. Med. 18, 761–768 (2012). https://doi.org/10.1007/s11655-011-0940-7

Xiang-ying Kong, Rong-tian Wang, Neng Tian, et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit[J]. Chinese Journal of Integrative Medicine, 2012,18(10):761-768.
Kong, Xy., Wang, Rt., Tian, N. et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit., Chin. J. Integr. Med. 18, 761–768 (2012). https://doi.org/10.1007/s11655-011-0940-7 DOI：

Xiang-ying Kong, Rong-tian Wang, Neng Tian, et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit[J]. Chinese Journal of Integrative Medicine, 2012,18(10):761-768. DOI： 10.1007/s11655-011-0940-7.

摘要

To investigate the effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae (Ach) on bone marrow stem cells (BMSCs) homing to necrosis area after osteonecrosis of the femoral head (ONFH) frozen by liquid nitrogen in rabbit as well as to explore the mechanism of prevention and treatment for ONFH. The animal model of ONFH was established by liquid nitrogen frozen on the rabbit left hind leg. Forty-eight Japanese White rabbits were randomly assigned to sham-operated group

model group

Huogu II group

and Huogu II plus Ach group

with 12 rabbits in each. During the course of ONFH animal model establishment

all rabbits were subcutaneously injected with recombinant human granulocyte colony-stimulating factor [rhG-CSF

30 μg/(kg·day) for continuous 7 days]. Meanwhile

normal saline and decoction of the two formulae were administrated by gavage

respectively. White blood cells (WBC) were counted in peripheral blood before and after injection of rhG-CSF. Materials were drawn on the 2nd and 4th weeks after model built; bone glutamine protein (BGP) and bone morphogenetic protein 2 (BMP2) levels in serum were tested. Histopathologic changes were observed by hematoxylin and eosin (HE) staining. BMP2 mRNA levels were detected with in situ hybridization (ISH) staining. 5-Bromo-2′-deoxyuridine (BrdU) and stromal cell derived factor 1 (SDF-1) were measured by immunohistochemical assay in femoral head of the left hind leg. Compared with the shamoperated group

the ratio of empty lacuna

serum BGP

and SDF-1 level in the model group increased significantly

and BMP2 in both serum and femoral head decreased significantly. However

in comparison with the model group

the empty lacuna ratio of Huogu II group and Huogu II plus Ach group decreased obviously in addition to the levels of serum BGP and BMP2

and the expressions of BMP2 mRNA

BrdU

and SDF-1 increased significantly. Above changes were particularly obvious in Huogu II plus Ach group. BGP and SDF-1 on the 2nd week and empty lacuna rate and serum BMP2 level on the 4th week in Huogu II group significantly exceeded their counterparts. On the 2nd week

only in Huogu II plus Ach group that the BrdU counting rose significantly. On the 4th week

empty lacuna rate and serum BMP2 level in Huogu II plus Ach group exceeded those in Huogu II group distinctively. To a certain extent

the medicinal guide Ach improves the preventive and therapeutic effects of Huogu II Formula on experimental ONFH model. The possible mechanism of this is related to its promoting effect on directional homing of BMSCs to the necrosis area.

Abstract

model group

Huogu II group

and Huogu II plus Ach group

with 12 rabbits in each. During the course of ONFH animal model establishment

all rabbits were subcutaneously injected with recombinant human granulocyte colony-stimulating factor [rhG-CSF

30 μg/(kg·day) for continuous 7 days]. Meanwhile

normal saline and decoction of the two formulae were administrated by gavage

the ratio of empty lacuna

serum BGP

and SDF-1 level in the model group increased significantly

and BMP2 in both serum and femoral head decreased significantly. However

in comparison with the model group

the empty lacuna ratio of Huogu II group and Huogu II plus Ach group decreased obviously in addition to the levels of serum BGP and BMP2

and the expressions of BMP2 mRNA

BrdU

only in Huogu II plus Ach group that the BrdU counting rose significantly. On the 4th week

empty lacuna rate and serum BMP2 level in Huogu II plus Ach group exceeded those in Huogu II group distinctively. To a certain extent

关键词

osteonecrosis of the femoral headbone marrow stem cellshomingguide drug to affected area

Keywords

osteonecrosis of the femoral headbone marrow stem cellshomingguide drug to affected area

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