Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats

Xiao-guang Xu; Hong Zhang; Xin-ling Bi; Jun Gu; Yu-ling Shi; Qiang Hou

doi:10.1007/s11655-012-0995-0

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Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats

OriginalPaper | Updated：2021-08-27

- Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats
- Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats
- 中国结合医学杂志（英文版） 2012年18卷第2期页码：137-145
- Affiliations：
  
  1. Department of Dermatology, Changhai Hospital, Second Military Medical University,Shanghai,China
  2. Dermatology Department of Traditional Chinese Medicine, Haici Hospital, Tsingtao,Shandong Province,China
  3. Department of Dermatology, the Tenth People’s Hospital, Tongji University,Shanghai,China
  4. Department of Dermatology, Dahua Hospital,Shanghai,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-012-0995-0
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-2-5，
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Xu, Xg., Zhang, H., Bi, Xl. et al. Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats., Chin. J. Integr. Med. 18, 137–145 (2012). https://doi.org/10.1007/s11655-012-0995-0

Xiao-guang Xu, Hong Zhang, Xin-ling Bi, et al. Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats[J]. Chinese Journal of Integrative Medicine, 2012,18(2):137-145.
Xu, Xg., Zhang, H., Bi, Xl. et al. Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats., Chin. J. Integr. Med. 18, 137–145 (2012). https://doi.org/10.1007/s11655-012-0995-0 DOI：

Xiao-guang Xu, Hong Zhang, Xin-ling Bi, et al. Xiaoyin Recipe (消银方) for psoriasis induces a Th1/Th2 balance drift toward Th2 in peripheral blood mononuclear cells of experimental autoimmune thyroiditis rats[J]. Chinese Journal of Integrative Medicine, 2012,18(2):137-145. DOI： 10.1007/s11655-012-0995-0.

摘要

To explore the regulatory mechanism of Xiaoyin Recipe (消银方) on the T helper 1/T helper 2 (Th1/Th2) immune balance. Thirty-six experimental animals were divided into three groups

12 rats in each group: blank control group (B group)

negative control group (N group)

and Xiaoyin Recipe treatment group (T group). The latter two groups received immunization of experimental autoimmune thyroiditis (EAT)

and T group were treated with Xiaoyin Recipe for a month. Then

the expression of Th1-Th2-related genes in peripheral blood mononuclear cells (PBMCs) were screened with Oligo GEArray Rat Th1-Th2-Th3 Microarray. The expressions of tumor necrosis factor-α (TNF-α)

interleukin-10 (IL-10)

T-box expressed in T-cells (T-bet)

and GATA-binding protein-3 (GATA-3) were detected by real-time polymerase chain reaction (RT-PCR). Gene array screening showed that compared to N group

in T group after Xiaoyin Recipe treatment

3 genes were upregulated in EAT rats

including interleukin-27 receptor alpha (IL-27rα)

glomulin (Glmn)

and GATA-3

while 38 genes were downregulated

such as CD28

IL-18

signal transducer

and activator of transcription 1 (STAT1)

T-bet

TNF receptor superfamily member 4 (TNFRSF4)

TNF ligand superfamily member 5 (TNFSF5)

and TNF receptor superfamily member 5 (TNFRSF5). While RT-PCR showed that there was an increased level of TNF-α mRNA (P<0.01)

an elevated ratio of T-bet/GATA-3

and a decreased level of IL-10 mRNA in PBMC of N and T group compared to B group (P <0.01); and after treatment with Xiaoyin Recipe

IL-10 mRNA level increased (P <0.01)

while TNF-α mRNA level and T-bet/GATA-3 ratio decreased in T group compared to N group (P <0.01). Xiaoyin Recipe for psoriasis could induce a Th1/Th2 balance drift toward Th2 in PBMC of EAT rats and thus improve the conditions.

Abstract

To explore the regulatory mechanism of Xiaoyin Recipe (消银方) on the T helper 1/T helper 2 (Th1/Th2) immune balance. Thirty-six experimental animals were divided into three groups

12 rats in each group: blank control group (B group)

negative control group (N group)

and Xiaoyin Recipe treatment group (T group). The latter two groups received immunization of experimental autoimmune thyroiditis (EAT)

and T group were treated with Xiaoyin Recipe for a month. Then

the expression of Th1-Th2-related genes in peripheral blood mononuclear cells (PBMCs) were screened with Oligo GEArray Rat Th1-Th2-Th3 Microarray. The expressions of tumor necrosis factor-α (TNF-α)

interleukin-10 (IL-10)

T-box expressed in T-cells (T-bet)

and GATA-binding protein-3 (GATA-3) were detected by real-time polymerase chain reaction (RT-PCR). Gene array screening showed that compared to N group

in T group after Xiaoyin Recipe treatment

3 genes were upregulated in EAT rats

including interleukin-27 receptor alpha (IL-27rα)

glomulin (Glmn)

and GATA-3

while 38 genes were downregulated

such as CD28

IL-18

signal transducer

and activator of transcription 1 (STAT1)

T-bet

TNF receptor superfamily member 4 (TNFRSF4)

TNF ligand superfamily member 5 (TNFSF5)

and TNF receptor superfamily member 5 (TNFRSF5). While RT-PCR showed that there was an increased level of TNF-α mRNA (P<0.01)

an elevated ratio of T-bet/GATA-3

and a decreased level of IL-10 mRNA in PBMC of N and T group compared to B group (P <0.01); and after treatment with Xiaoyin Recipe

IL-10 mRNA level increased (P <0.01)

关键词

experimental autoimmune thyroiditispsoriasisTh1Th2herbal medicineXiaoyin Recipe

Keywords

experimental autoimmune thyroiditispsoriasisTh1Th2herbal medicineXiaoyin Recipe

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