Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses

Yu-hua Jia; Yun-xian Zhang; Li-jun Li; Ya-wei Liu; Chun-hua Li; Xiu-qiong Fu; Ping Zeng; Wei-kang Wu; Xue-gang Sun

doi:10.1007/s11655-012-0998-x

Your Location：

Home >

Browse articles >

Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses

OriginalPaper | Updated：2021-08-27

- Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses
- Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses
- 中国结合医学杂志（英文版） 2012年18卷第2期页码：120-129
- Affiliations：
  
  1. The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University,Guangzhou,China
  2. Fuda Cancer Hospital,Guangzhou,China
  3. Guangdong General Hospital, Guangdong Academy of Medical Sciences,Guangzhou,China
  4. The Institute of Integrated Traditional Chinese and Western Medicine, Sun Yatsen University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30572435 and No. 81072776) and Baiyun District Science and Technology Program (No. 2010-KZ-39)
- DOI：10.1007/s11655-012-0998-x
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-2-5，
Scan for full text
Jia, Yh., Zhang, Yx., Li, Lj. et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses., Chin. J. Integr. Med. 18, 120–129 (2012). https://doi.org/10.1007/s11655-012-0998-x

Yu-hua Jia, Yun-xian Zhang, Li-jun Li, et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses[J]. Chinese Journal of Integrative Medicine, 2012,18(2):120-129.
Jia, Yh., Zhang, Yx., Li, Lj. et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses., Chin. J. Integr. Med. 18, 120–129 (2012). https://doi.org/10.1007/s11655-012-0998-x DOI：

Yu-hua Jia, Yun-xian Zhang, Li-jun Li, et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses[J]. Chinese Journal of Integrative Medicine, 2012,18(2):120-129. DOI： 10.1007/s11655-012-0998-x.

摘要

To identify the underlying mechanisms of the protective effects of Dingxin Recipe (定: 心方

DXR)

a Chinese compound prescription that has been used clinically in China for more than 20 years

on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. A total of 30 rats were randomly divided into three groups: sham group

I/R group

and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXR-DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days

while rats I/in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrix-matrixassisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify assisted differentially expressed proteins. Immunohistochemistry

real-time quantitative polymerase chain reaction (RQ-RQPCR)

Western blot

and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins PCR)

obtained in the above experiments. DXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality

as well as arrhythmia score

compared with those of the I/R group. Among successfully identified proteins

prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition

compared with the I/R group

the level of glutathione (GSH) was elevated accompanied by reduced expressions of interleukin-6 (IL-6) and neutrophil infiltration in I/R rats with DXR pretreatment. DXR could alleviate I/R-induced arrhythmias

which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore

up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.

Abstract

To identify the underlying mechanisms of the protective effects of Dingxin Recipe (定: 心方

DXR)

a Chinese compound prescription that has been used clinically in China for more than 20 years

on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. A total of 30 rats were randomly divided into three groups: sham group

I/R group

and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXR-DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days

real-time quantitative polymerase chain reaction (RQ-RQPCR)

Western blot

and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins PCR)

obtained in the above experiments. DXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality

as well as arrhythmia score

compared with those of the I/R group. Among successfully identified proteins

prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition

compared with the I/R group

which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore

up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.

关键词

Dingxin Recipeproteomicsarrhythmiasprohibitinischemia/reperfusioninflammation

Keywords

Dingxin Recipeproteomicsarrhythmiasprohibitinischemia/reperfusioninflammation

references

Gheorghiade M, Bonow RO. Chronic heart failure in the United States: a manifestation of coronary artery disease. Circulation 1998;97:282–289.

Meldrum DR. Tumor necrosis factor in the heart. Am J Physiol Regul Integr Comp Physiol 1998;274:R577–R595.

Chandrasekar B, Mitchell DH, Colston JT, Freeman GL. Regulation of CCAAT/Enhancer binding protein, interleukin-6, interleukin-6 receptor, and gp130 expression during myocardial ischemia/reperfusion. Circulation 1999;99:427–433.

Kukielka GL, Smith CW, LaRosa GJ, Manning AM, Mendoza LH, Daly TJ, et al. Interleukin-8 gene induction in the myocardium after ischemia and reperfusion in vivo. J Clin Invest 1995;95:89–103.

Lucchesi BR, Kilgore KS. Complement inhibitors in myocardial ischemia/reperfusion injury. Immunopharmacology 1997;38:27–42.

Vinten-Johansen J, Jiang R, Reeves JG, Mykytenko J, Deneve J, Jobe LJ. Inflammation, proinflammatory mediators and myocardial ischemia-reperfusion injury. Hematol Oncol Clin North Am 2007;21:123–145.

Albertine KH, Weyrich AS, Ma XL, Lefer DJ, Becker LC, Lefer AM. Quantification of neutrophil migration following myocardial ischemia and reperfusion in cats and dogs. J Leukoc Biol 1994;55:557–566.

Lefer AM, Ma XL, Weyrich A, Lefer DJ. Endothelial dysfunction and neutrophil adherence as critical events in the development of reperfusion injury. Agents Actions Suppl 1993;4:127–135.

Chen JK, Chow SE. Antioxidants and myocardial ischemia: reperfusion injuries. Chang Gung Med J 2005;28:369–377.

Jia YH, Sun XG, Jia MY, Cui ZY, Li CX. Observation of the effects of Dingxin Recipe on 145 tachyarrhythmia patients. J Pract Tradit Chin Med (Chin) 1999;15:3–4.

Jia YH, Yu LZ, Chen YY, Zhou YL, Sun XG. Effect of Dingxin Recipe on arrhythmia and injury induced by ischemia and reperfusion in rabbits. Chin J Integr Tradit West Med (Chin) 1999;19:678–681.

Jia YH, Chen YY, Zhou YL, Sun XG. A. Effects of Dingxin Recipe on arrhythmias induced by ischemia/reperfusion. Chin Gen Pract (Chin) 1999;2:126–128.

Zhao XS, Jia YH, Jiang Y, Luo R, Qin QH. Effects of Dingxin Recipe on p38 mitogen activated protein kinase and tumor necrosis factor alpha of myocardial ischemia reperfusion injury in rats. Chin J Clin Rehabil (Chin) 2004;8:2882–2884.

Jia YH, Zhang YX, Zhou YP, Sun XG. Effects of Dingxin Recipe and oxymatrine on reperfusion arrhythmias with rats and expression of ICAM-1. Liaoning J Tradit Chin Med (Chin) 2008;35:32–34.

Jia YH, Sun XG, Chen YY. Effects of Dingxin Recipe and tanshinose II A on expression of platelet membrane adhesive molecule in the rat of arrhythmia. J Tradit Chin Med (Chin) 2002;43:140–142.

Jia YH, Zhou YP, Chen YY, Zhang YX, Sun XG. Protective effect of Dingxin Formula on arrhythmia rats with myocardial ischemical reperfusion injury and its effect on NF-κB activation. J Tradit Chin Med (Chin) 2007;48:357–359.

Himori N, Matsuura A. A simple technique for occlusion and reperfusion of coronary artery in conscious rats. Am J Physiol 1989;256:H1719–H1725.

Walker MJ, Curtis MJ, Hearse DJ, Campbell RW, Janse MJ, Yellon DM, et al. The Lambeth Conventions: guidelines for the study of arrhythmias in ischaemia infarction, and reperfusion. Cardiovasc Res 1988;22:447–455.

Leprán I, Baczkó I, Varró A, Papp JG. ATP-sensitive potassium channel modulators: both pinacidil and glibenclamide produce antiarrhythmic activity during acute myocardial infarction in conscious rats. J Pharmacol Exp Ther 1996;277:1215–1220.

Guo R, Gao XY, Wang W, Wang HJ, Zhang F, Zhang Y, et al. Tempol reduces reperfusion-induced arrhythmias in anaesthetized rats. Pharmacol Res 2005;52:192–198.

Fishbein MC, Meerbaum S, Rit J, Lando U, Kanmatsuse K, Mercier JC, et al. Early phase acute myocardial infarct size quantification: validation of the triphenyl tetrazolium chloride tissue enzyme staining technique. Am Heart J 1981;101:593–600.

Liu L, Zhao L, Zhang Y, Zhang Q, Ding Y. Proteomic analysis of Tiam1-mediated metastasis in colorectal cancer. Cell Biol Int 2007;31:805–814.

de Jonge N, van Wichen DF, Schipper ME, Lahpor JR, Gmelig-Meyling FH, Robles de Medina EO, et al. Left ventricular assist device in end-stage heart failure: persistence of structural myocyte damage after unloading. An immunohistochemical analysis of the contractile myofilaments. J Am Coll Cardiol 2002;39:963–969.

Song G, Zhao X, Xu J, Song H. Increased expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in rat cardiac allografts. Transplant Proc 2008;40:2720–2723.

Li M, Georgakopoulos D, Lu G, Hester L, Kass DA, Hasday J, et al. p38 MAP kinase mediates inflammatory cytokine induction in cardiomyocytes and extracellular matrix remodeling in heart. Circulation 2005;111:2494–2502.

Acevedo M, Corbalán R, Braun S, Pereira J, Navarrete C, Gonzalez I. C-reactive protein and atrial fibrillation: “evidence for the presence of inflammation in the perpetuation of the arrhythmia.” Int J Cardiol 2006;108:326–331.

Engelman DT, Watanabe M, Maulik N, Cordis GA, Engelman RM, Rousou JA, et al. L-arginine reduces endothelial inflammation and myocardial stunning during ischemia/reperfusion. Ann Thorac Surg 1995;60:1275–1281.

Li XL, Chu WM, Liu JL. Antiarrhythmic properties of long-term treatment with matrine in arrhythmic rat induced by coronary ligation. Biol Pharm Bull 2009;32:1521–1526.

Huang WM, Wu ZD, Gan YQ. Effects of berberine on ischemic ventricular arrhythmia. Chin J Cardiol 1989;17:300–301, 319.

Sun DD, Wang HC, Wang XB, Luo Y, Jin ZX, Li ZC, et al. Tanshinone II A: a new activator of human cardiac KCNQ1/KCNE1 (Iks) potassium channels. Eur J Pharmacol 2008;590:317–321.

Zhang LH, Jia YH, Sun XG, Xing FY, Liu ZF, Song G, et al. Effects of total Panax notoginseng saponins on the activity of human nitric oxide synthase gene promotor. J First Milit Med Univ (Chin) 2004;24:1113–1116.

Gao BY, Li XJ, Liu L, Zhang BH. Effect of panaxatriol saponins isolated from Panax notoginseng (PTS) on myocardial ischemic arrhythmia in mice and rats. Acta Pharm Sin (Chin) 1992;27:641–644.

Chen XJ, Yu CL, Liu JF. Protective effects of total saponins of semen Ziziphi spinosae on cultured rat myocardial cells. Acta Pharmacol Sin 1990;11:153–155.

Lai KN, Chan LY, Tang SC, Leung JC. Ganoderma extract prevents albumin-induced oxidative damage and chemokines synthesis in cultured human proximal tubular epithelial cells. Nephrol Dial Transplant 2006;21:1188–1197.

Jia YH, Sun XG, Zhao XS. Effect of Dingxin Recipe on intracellular free calcium, membrane potential and mitochondria membrane potential in normal and hypoxia myocytes of rats. Chin J Integr Tradit West Med (Chin) 2001;21:680–683.

Knowlton AA, Apstein CS, Saouf R, Brecher P. Leakage of heart fatty acid binding protein with ischemia and reperfusion in the rat. J Mol Cell Cardiol 1989;21:577–583.

Glatz JF, van Bilsen M, Paulussen RJ, Veerkamp JH, van der Vusse GJ, Reneman RS. Release of fatty acid-binding protein from isolated rat heart subjected to ischemia and reperfusion or to the calcium paradox. Biochim Biophys Acta 1988;961:148–152.

Ikonen E, Fiedler K, Parton RG, Simons K. Prohibitin, an antiproliferative protein, is localized to mitochondria. FEBS Lett 1995;358:273–277.

Kasashima K, Sumitani M, Satoh M, Endo H. Human prohibitin 1 maintains the organization and stability of the mitochondrial nucleoids. Exp Cell Res 2008;314:988–996.

Nuell MJ, Stewart DA, Walker L, Friedman V, Wood CM, Owens GA, et al. Prohibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells. Mol Cell Biol 1991;11:1372–1381.

Terashima M, Kim KM, Adachi T, Nielsen PJ, Reth M, Köhler G, et al. The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein. EMBO J 1994;13:3782–3792.

Rajalingam K, Rudel T. Ras-Raf signaling needs prohibitin. Cell cycle 2005;4:1503–1505.

Coates PJ, Jamieson DJ, Smart K, Prescott AR, Hall PA. The prohibitin family of mitochondrial proteins regulate replicative lifespan. Curr Biol 1997;7:607–610.

Liu X, Ren Z, Zhan R, Wang X, Wang X, Zhang Z, et al. Prohibitin protects against oxidative stress-induced cell injury in cultured neonatal cardiomyocyte. Cell Stress Chaperones 2009;14:311–319.

Theiss AL, Idell RD, Srinivasan S, Klapproth JM, Jones DP, Merlin D, et al. Prohibitin protects against oxidative stress in intestinal epithelial cells. FASEB J 2007;21:197–206.

Theiss AL, Jenkins AK, Okoro NI, Klapproth JM, Merlin D, Sitaraman SV. Prohibitin inhibits tumor necrosis factor alpha-induced nuclear factor-kappa B nuclear translocation via the novel mechanism of decreasing importin α3 expression. Mol Biol Cell 2009;20:4412–4423.

Daemen MA, van’t Veer C, Denecker G, Heemskerk VH, Wolfs TG, Lauss CM, et al. Inhibition of apoptosis induced by ischemia-reperfusion prevents inflammation. J Clin Invest 1999;104:541–549.

Zhou YP, Jia YH, Yang P, Chen YY, Zhang YX, Sun XG. The effects of Dingxin Recipe on myocardial apoptosis in ischemia/reperfusion induced arrhythmias in rats. Chin J Basic Med Tradit Chin Med (Chin) 2007;13:515–516.

Fusaro G, Wang S, Chellappan S. Differential regulation of Rb family proteins and prohibitin during camptothecin-induced apoptosis. Oncogene 2002;21:4539–4548.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

The contribution of deer velvet antler research to the modern biological medicine

Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs

Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics

Effect of safflor yellow injection on inhibiting lipopolysaccharide-induced pulmonary inflammatory injury in mice

Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection