Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats

Shu-yu Yang; Neng-jiang Zhao; Xue-jun Li; Hui-jie Zhang; Ke-ji Chen; Can-dong Li

doi:10.1007/s11655-012-1023-0

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Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats

OriginalPaper | Updated：2021-08-27

- Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats
- Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats
- 中国结合医学杂志（英文版） 2012年18卷第4期页码：262-268
- Affiliations：
  
  1. Post-Graduate School, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Department of Internal Medicine, the First Affiliated Hospital of Xiamen University,Fujian Province,Xiamen,China
  3. Xiamen Diabetes Institute,Fujian Province,Xiamen,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30973912);and the Innovation Project of Fujian Provincial Health Bureau (No. 2007-CXB-10);and the Project of Technology Bureau of Xiamen City (No. 3502Z20077050)
- DOI：10.1007/s11655-012-1023-0
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-3-30，
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Yang, Sy., Zhao, Nj., Li, Xj. et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats., Chin. J. Integr. Med. 18, 262–268 (2012). https://doi.org/10.1007/s11655-012-1023-0

Shu-yu Yang, Neng-jiang Zhao, Xue-jun Li, et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats[J]. Chinese Journal of Integrative Medicine, 2012,18(4):262-268.
Yang, Sy., Zhao, Nj., Li, Xj. et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats., Chin. J. Integr. Med. 18, 262–268 (2012). https://doi.org/10.1007/s11655-012-1023-0 DOI：

Shu-yu Yang, Neng-jiang Zhao, Xue-jun Li, et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats[J]. Chinese Journal of Integrative Medicine, 2012,18(4):262-268. DOI： 10.1007/s11655-012-1023-0.

摘要

To investigate the therapeutic effects of Ping-tang Recipe (平糖方

PTR) on high-fat diet (HFD)-induced insulin resistance and non-alcoholic fatty liver disease (NAFLD)

and to elucidate the underlying mechanisms. Forty male SD rats were included in the study. Ten rats were fed on normal diet as normal control

and thirty rats were fed on HFD for 8 weeks to induce obesity

followed with low dose (0.42 g/kg) or high dose (0.84 g/kg) of PTR or vehicle for 8 weeks with 10 animals for each group. Glucose metabolism and insulin sensitivity were evaluated by oral glucose tolerance test and insulin tolerance test. Hepatic steatosis was measured by immunohistochemistry. Liver lipid metabolic genes were analyzed by quantitative real-time polymerase chain reaction

while AMP-activated protein kinase (AMPK) expression was examined by Western blot. Rats fed on HFD developed abdominal obesity

insulin resistance and NAFLD. PTR treatment reduced visceral fat (peri-epididymal and peri-renal) accumulation

improved glucose metabolism

and attenuated hepatic steatosis. The expressions of the key lipolytic regulating genes

including peroxisome proliferators-activated receptor γ co-activator 1α (PGC-1α)

peroxisome proliferator-activated receptor γ (PRAR-γ) and α (PRAR-α)

were up-regulated (P<0.05 or P<0.01)

while the expressions of lipogenic genes such as sterol regulatory element-binding protein 1c (SREBP-1c)

fatty acid synthase (FAS) and liver fatty acid-binding protein (L-FABP) were down-regulated (P<0.05 or P<0.01). In addition

PTR activated AMPK and promoted acetyl-CoA carboxylase phosphorylation in the liver. PTR improves insulin resistance and reverse hepatic steatosis in the rat model of HFD-induced obesity through promotion of lipolysis and reduction of lipogenesis

which involves the AMPK signaling pathway

thus representing a new therapeutic intervention for obesity related insulin resistance and NAFLD.

Abstract

To investigate the therapeutic effects of Ping-tang Recipe (平糖方

PTR) on high-fat diet (HFD)-induced insulin resistance and non-alcoholic fatty liver disease (NAFLD)

and to elucidate the underlying mechanisms. Forty male SD rats were included in the study. Ten rats were fed on normal diet as normal control

and thirty rats were fed on HFD for 8 weeks to induce obesity

while AMP-activated protein kinase (AMPK) expression was examined by Western blot. Rats fed on HFD developed abdominal obesity

insulin resistance and NAFLD. PTR treatment reduced visceral fat (peri-epididymal and peri-renal) accumulation

improved glucose metabolism

and attenuated hepatic steatosis. The expressions of the key lipolytic regulating genes

including peroxisome proliferators-activated receptor γ co-activator 1α (PGC-1α)

peroxisome proliferator-activated receptor γ (PRAR-γ) and α (PRAR-α)

were up-regulated (P<0.05 or P<0.01)

while the expressions of lipogenic genes such as sterol regulatory element-binding protein 1c (SREBP-1c)

fatty acid synthase (FAS) and liver fatty acid-binding protein (L-FABP) were down-regulated (P<0.05 or P<0.01). In addition

which involves the AMPK signaling pathway

thus representing a new therapeutic intervention for obesity related insulin resistance and NAFLD.

关键词

Ping-tang Recipenon-alcoholic fatty liver diseaseinsulin resistanceAMP-activated protein kinase

Keywords

Ping-tang Recipenon-alcoholic fatty liver diseaseinsulin resistanceAMP-activated protein kinase

references

Chitturi S, Wong VW, Farrell G. Nonalcoholic fatty liver in Asia: firmly entrenched and rapidly gaining ground. J Gastroenterol Hepatol 2011;26(Suppl):163–172.

Alper AT, Hasdemir H, Sahin S, Ontürk E, Akyol A, Nurkalem Z, et al. The relationship between nonalcoholic fatty liver disease and the severity of coronary artery disease in patients with metabolic syndrome. Turk Kardiyol Dern Ars 2008;36:376–381.

Sanyal AJ. AGA technical review on nonalcoholic fatty liver disease. Gastroenterology 2002;123:1705–1725.

Ratziu V, Caldwell S, Neuschwander-Tetri BA. Therapeutic trials in nonalcoholic steatohepatitis: insulin sensitizers and related methodological issues. Hepatology 2010;52:2206–2215.

Tsochatzis EA, Papatheodoridis GV. Is there any progress in the treatment of non-alcoholic fatty liver disease? World J Gastrointest Pharmacol Ther 2011;2;1–5.

Zhang HX, Yang SY, Cao HX. Effect of Pingtang Recipe containing drug-serum on INS-1 pancreatic beta cells lipoapoptosis. Chin J Integr Tradit West Med (Chin) 2010;30;978–981.

Li XJ, Yang SY. Clinical observation on treatment of diabetes mellitus type 2 by Pingtang concentrated capsule. Chin J Integr Tradit West Med (Chin) 2002;22:854–855.

Lee MS, Kim D, Jo K, Hwang JK. Nordihydroguaiaretic acid protects against high-fat diet-induced fatty liver by activating AMP-activated protein kinase in obese mice. Biochem Biophys Res Commun 2010;401:92–97.

Ha SK, Chae C. Inducible nitric oxide distribution in the fatty liver of a mouse with high fat diet-induced obesity. Exp Anim 2010;59:595–604.

Kucera O, Garnol T, Lotková H, Staňková P, Mazurová Y, Hroch M, et al. The effect of rat strain, diet composition and feeding period on the development of a nutritional model of non-alcoholic fatty liver disease in rats. Physiol Res 2011;60;317–328.

Orlik B, Handzlik G, Olszanecka-Glinianowicz M. The role of adipokines and insulin resistance in the pathogenesis of nonalcoholic fatty liver disease. Postepy Hig Med Dosw 2010;64;212–219.

Qin Y, Tian YP. Preventive effects of chronic exogenous growth hormone levels on diet-induced hepatic steatosis in rats. Lipids Health Dis 2010;9;78.

Matsumoto M, Ogawa W, Akimoto K, Inoue H, Miyake K, Furukawa K, et al. PKClambda in liver mediates insulininduced SREBP-1c expression and determines both hepatic lipid content and overall insulin sensitivity. J Clin Invest 2003;112;935–944.

Cao H, Maeda K, Gorgun CZ, Kim HJ, Park SY, Shulman GI, et al. Regulation of metabolic responses by adipocyte/macrophage fatty acid-binding proteins in leptin-deficient mice. Diabetes 2006;55;1915–1922.

Liang H, Ward WF. PGC-1alpha, a key regulator of energy metabolism. Adv Physiol Educ 2006;30;145–151.

Gruzman A, Babai G, Sasson S. Adenosine monophosphate-activated protein kinase (AMPK) as a new target for antidiabetic drugs: a review on metabolic, pharmacological and chemical considerations. Rev Diabet Study 2009;6:13–36.

Barroso E, Rodríguez-Calvo R, Serrano-Marco L, Astudillo AM, Balsinde J, Palomer X, et al. The PPARβ/δ activator GW501516 prevents the down-regulation of AMPK caused by a high-fat diet in liver and amplifies the PGC-1α-lipin 1-PPARα pathway leading to increased fatty acid oxidation. Endocrinology 2011;152:1848–1859.

Yuan HD, Yuan HY, Chung SH, Jin GZ, Piao GC. An active part of Artemisia sacrorum Ledeb. attenuates hepatic lipid accumulation through activating AMP-activated protein kinase in human HepG2 cells. Biosci Biotechnol Biochem 2010;74;322–328.

Canto C, Auwerx J. PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Curr Opin Lipidol 2009;20;98–105.

Zhang BB, Zhou G, Li C. AMPK: an emerging drug target for diabetes and the metabolic syndrome. Cell Metab 2009;9;407–416.

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