Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄 虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells

Na Liu; Jun-tian Liu; Yuan-yuan Ji; Pei-pei Lu

doi:10.1007/s11655-012-1033-y

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Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells

OriginalPaper | Updated：2021-08-27

- Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells
- Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells
- 中国结合医学杂志（英文版） 2013年19卷第6期页码：432-438
- Affiliations：
  
  1. Department of Pharmacology, Xi’an Jiaotong University School of Medicine,Xi’an,China
  2. Scientific Research and Experiment Center, the Second Affiliated Hospital, Xi’an Jiaotong University School of Medicine,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30572347)
- DOI：10.1007/s11655-012-1033-y
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2012-4-11，
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Liu, N., Liu, Jt., Ji, Yy. et al. Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells., Chin. J. Integr. Med. 19, 432–438 (2013). https://doi.org/10.1007/s11655-012-1033-y

Na Liu, Jun-tian Liu, Yuan-yuan Ji, et al. Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells[J]. Chinese Journal of Integrative Medicine, 2013,19(6):432-438.
Liu, N., Liu, Jt., Ji, Yy. et al. Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells., Chin. J. Integr. Med. 19, 432–438 (2013). https://doi.org/10.1007/s11655-012-1033-y DOI：

Na Liu, Jun-tian Liu, Yuan-yuan Ji, et al. Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells[J]. Chinese Journal of Integrative Medicine, 2013,19(6):432-438. DOI： 10.1007/s11655-012-1033-y.

摘要

To investigate and compare the effects and mechanisms of three functional parts of Dahuang Zhechong Pill (大黄虫丸

DHZCP)

including drugs with the function of removing blood stasis and promoting blood circulation (FP-I)

drugs with the function of expelling heat and moistening dryness (FP-II)

and drugs with the function of nourishing yin and replenishing blood (FP-III) of DHZCP

on platelet-derived growth factor (PDGF)-stimulated vascular smooth muscle cells (VSMCs) proliferation with the method of serum pharmacology. VSMCs proliferation of rat was assayed by measuring the cell viability with the 3-(4

5-dimethylthiazol-2yl)-2

5-diphenyltetrazolium bromide (MTT) method. DNA synthesis in VSMCs was examined by detecting 5′-bromo-2′-deoxyuridine incorporation with the immunocytochemical method. Cycle of VSMCs was evaluated with flow cytometry. Expression of cyclin D1

p27

PKCα

and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) was quantified by the Western blotting method. The FP-I and FP-III containing serum was capable of inhibiting PDGF-stimulated proliferation and DNA synthesis of VSMCs

arrested VSMCs in G phase

downregulated cyclin D1

and upregulated p27 expression (P <0.01 or P <0.05). The FP-I and FP-III containing serum also inhibited the PDGF-induced phosphorylation of tyrosine of ERK1/2 and PKCα expression (P <0.01 or P <0.05). FP-I and FP-III of DHZCP are able to inhibit VSMCs proliferation via interrupting PKCα-ERK1/2 signaling

modulating the expression of cell cycle proteins to result in arresting the cells in G phase. The inhibitory effect is mainly related to the function of removing blood stasis and promoting blood circulation

slightly to the function of nourishing yin and replenishing blood

but not to the function of expelling heat and moistening dryness.

Abstract

To investigate and compare the effects and mechanisms of three functional parts of Dahuang Zhechong Pill (大黄虫丸

DHZCP)

including drugs with the function of removing blood stasis and promoting blood circulation (FP-I)

drugs with the function of expelling heat and moistening dryness (FP-II)

and drugs with the function of nourishing yin and replenishing blood (FP-III) of DHZCP

5-dimethylthiazol-2yl)-2

p27

PKCα

arrested VSMCs in G phase

downregulated cyclin D1

slightly to the function of nourishing yin and replenishing blood

but not to the function of expelling heat and moistening dryness.

关键词

Dahuang Zhechong Pillproliferationcell cyclecyclin D1p27

Keywords

Dahuang Zhechong Pillproliferationcell cyclecyclin D1p27

references

Libby P. Inflammat ion in atherosclerosis. Nature 2002;420:868–874.

Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993;362:801–809.

Ross R. Cell biology of atherosclerosis. Annu Rev Physiol 1995;57:791–804.

Sachinidis A, Locher R, Vetter W, Tatje D, Hoppe J. Different effects of platelet-derived growth factor isoforms on rat vascular smooth muscle cells. J Biol Chem 1990;265:10238–10243.

Yan JJ, Chen XY. The progress of experiment study on Dahuang Zhechong Pill. Chin J Exp Tradit Med Formul (Chin) 2000;6:60–61.

Chen JF. Efficacy of Dahuang Zhechong Pill in treatment of 50 cases of patients with carotid atherosclerosis. Shandong J Tradit Chin Med (Chin) 2001;20:331–332.

Li WL, Wu SP. Clinical effect of Dahuang Zhechong Pill on chronic hepatitis with hepatic fibrosis. China Pharm (Chin) 2008;19:1658–1659.

Yuan HX. Clinical application of Dahuang Zhechong Pill in gynecology. J Chin Integr Med (Chin) 2009;7:168–170.

Li JL, Liu JT, Gou W, Li XK, Liu CH. Effect of Dahuang Zhechong Pill on vascular smooth muscle cells in atherosclerotic rabbits. Chin Tradit Patent Med (Chin) 2006;28:1470–1472.

Zhang YH, Liu JT, Wen BY, Xiao XH. In vitro inhibition of proliferation of vascular smooth muscle cells by serum of rats treated with Dahuang Zhechong Pill. J Ethnopharmacol 2007;112:375–379.

Zhang YH, Liu JT, Wen BY, Liu N. Mechanisms of inhibiting proliferation of vascular smooth muscle cells by serum of rats treated with Dahuang Zhechong Pill. J Ethnopharmacol 2009;124:125–129.

Tashino S. “Serum pharmacology” and “serum pharmaceutical chemistry”: from pharmacology of Chinese traditional medicines to start a new measurement of drug concentration in blood. Ther Drug Monit Res 1988;5:54–64.

Skalli O. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 1986;103:2787–2796.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytoxicity assays. J Immunol Methods 1983;95:55–63.

Gratzner HG. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 1982;218:474–475.

Darzynkiewicz Z, Bedner E, Smolewski P. Flow cytometry in analysis of cell cycle and apoptosis. Semin Hematol 2001;38:179–193.

Blake MS, Johnston KH, Russell-Jones GJ, Gotschlich EC. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem 1984;136:175–179.

Meng YC, Ding X, Ben CE. The application view of Chinese traditional medicine serum pharmacology. J Beijing Univ Tradit Chin Med (Chin) 1999;22:42–43.

Lavoie JN, Allemain GL, Brunet A, Muller R, Pouyssegur J. Cyclin D1 expression is regulated positively by the p42/ p44MAPK and negatively by the p38/HOGMAPK pathway. J Biol Chem 1996;71:20608–20616.

Sherr CJ. G1 phase progression: cycling on cue. Cell 1994;79:551–555.

Morgan DO. Principles of CDK regulation. Nature 1995;374:131–134.

Sherr CJ, Roberts JM. CDK inhibitors: positive and negative regulators of G1 phase progression. Gene Dev 1999;13:1501–1512.

Tanner FC, Yang ZY, Duckers E, Gordon D, Nabel GJ, Nabel EG. Expression of cyclin-dependent kinase inhibitors in vascular disease. Circ Res 1998;82:396–403.

Graf K, Xi XP, Yang D, Fleck E, Hsueh WA, Law RE. Mitogen-activated protein kinase activation is involved in platelet-derived growth factor-directed migration by vascular smooth muscle cells. Hypertension 1997;29:334–339.

Boulton TG, Nye SH, Robbins DJ, Ip NY, Radziejewska E, Morgenbesser SD, et al. ERKs: a family of proteinserine/ threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF. Cell 1991;65:663–675.

Wilkinson MG, Millar JBA. Control of the eukaryotic cell cycle by MAP kinase signaling pathways. FASEB J 2000;14:2147–2157.

Saito Y, Hojo Y, Tanimoto T, Abe J, Berk BC. Protein kinase C-α and protein kinase C-ɛ are required for Grb2-associated binder-1 tyrosine phosphorylation in response to plateletderived growth factor. J Biol Chem 2002;277:23216–23222.

Okazaki J, Mawatari K, Liu B, Kent KC. The effect of protein kinase C and its alpha subtype on human vascular smooth muscle cell proliferation, migration and fibronectin production. Surgery 2000;128:192–197.

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