Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid

Cheng-liang Wu; Qiang Mao; Hong-ting Jin; Lu-wei Xiao; Pei-jian Tong

doi:10.1007/s11655-012-1051-9

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Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid

OriginalPaper | Updated：2021-08-27

- Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid
- Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid
- 中国结合医学杂志（英文版） 2012年18卷第4期页码：269-275
- Affiliations：
  
  1. Zhejiang Traditional Chinese Medicine University,Hangzhou,China
  2. Zhejiang Provincial Institute of Ortopaedics and Traumatology,Hangzhou,China
  3. Ortopaedics and Traumatology Department of Zhejiang Provincial Traditional Chiese Medicine Hospital, the First Affiliated Hospital of Zhejiang Traditional Chinese Medicine University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30672702, No. 81072820 and No. 30873276) and Key Program of Traditional Chinese Medicine of Zhejiang Province (No. 2005Z003)
- DOI：10.1007/s11655-012-1051-9
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-3-30，
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Wu, Cl., Mao, Q., Jin, Ht. et al. Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid., Chin. J. Integr. Med. 18, 269–275 (2012). https://doi.org/10.1007/s11655-012-1051-9

Cheng-liang Wu, Qiang Mao, Hong-ting Jin, et al. Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid[J]. Chinese Journal of Integrative Medicine, 2012,18(4):269-275.
Wu, Cl., Mao, Q., Jin, Ht. et al. Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid., Chin. J. Integr. Med. 18, 269–275 (2012). https://doi.org/10.1007/s11655-012-1051-9 DOI：

Cheng-liang Wu, Qiang Mao, Hong-ting Jin, et al. Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid[J]. Chinese Journal of Integrative Medicine, 2012,18(4):269-275. DOI： 10.1007/s11655-012-1051-9.

摘要

To observe the regulation of Youguiyin (YGY

右归饮) on the gene expression profile of the rat with steroid-induced femoral head necrosis (sFHN)

for the sake of investigating its molecular mechanism of sFHN prevention and treatment. All the 30 rats were randomly divided into three groups

the normal control group (A)

the model control group (B)

and the YGY treated group (C)

10 in each group. After rats in Groups B and C were being made into FHN models with steroid injection

they received a daily intragastric administration of saline and YGY respectively in equal volume for a total of 6 weeks

while to the unmodeled normal rats in Group A

saline was administered instead. The rats were sacrificed at the terminal of administration; their mRNA from femoral head tissue was extracted and prepared to cDNA probe through inverse transcription for detecting gene expression profile by microarray

outcomes of which was passing fluorescence quantitative PCR verification

and the differential expressed genes were analyzed adopting gene ontology (GO) method. Compared with Group A

the numbers of differential genes found in Groups B and C were 190 and 92

respectively

but the changing trend in the two groups was opposite

mainly manifested as down-regulating in Group B/Group A (GB/GA) and up-regulating in Group C/Group B (GC/GB). The analysis showed that these differential genes were mainly assigned to cell apoptosis

signal transduction

metabolism

cell proliferation and differentiation

cell cycle

blood coagulation

antioxidant activity

etc. sFHN was regulated by various genes; the regulation of YGY on expressions of these genes and the intra/extra-cellular signaling processes was possibly the molecular mechanism of YGY for preventing/treating sFHN. This study gave an explanation to the effectiveness of Chinese medicine in preventing/treating FHN from aspects of gene expression and enriched the Chinese medicine theory of “Kidney (Shen) governing bones”.

Abstract

To observe the regulation of Youguiyin (YGY

右归饮) on the gene expression profile of the rat with steroid-induced femoral head necrosis (sFHN)

for the sake of investigating its molecular mechanism of sFHN prevention and treatment. All the 30 rats were randomly divided into three groups

the normal control group (A)

the model control group (B)

and the YGY treated group (C)

10 in each group. After rats in Groups B and C were being made into FHN models with steroid injection

they received a daily intragastric administration of saline and YGY respectively in equal volume for a total of 6 weeks

while to the unmodeled normal rats in Group A

outcomes of which was passing fluorescence quantitative PCR verification

and the differential expressed genes were analyzed adopting gene ontology (GO) method. Compared with Group A

the numbers of differential genes found in Groups B and C were 190 and 92

respectively

but the changing trend in the two groups was opposite

mainly manifested as down-regulating in Group B/Group A (GB/GA) and up-regulating in Group C/Group B (GC/GB). The analysis showed that these differential genes were mainly assigned to cell apoptosis

signal transduction

metabolism

cell proliferation and differentiation

cell cycle

blood coagulation

antioxidant activity

关键词

Youguiyinsteroid-induced femoral head necrosisgene chipgene expression profilemolecular mechanism

Keywords

Youguiyinsteroid-induced femoral head necrosisgene chipgene expression profilemolecular mechanism

references

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Wang YJ, Shi Q, Lu WW, Cheung KCM, Darowish M, Li TF, et al. Cervical intervertebral disc degeneration induced by unbalanced dynamic and static forces: a novel in vivo rat model. Spine 2006;31:1532–1538.

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