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A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector
A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector
- 中国结合医学杂志（英文版） 2012年18卷第9期 页码：690-698
- Affiliations：
1. Institute of Integrated Traditional Chinese and Western Medicine, and the National Key Clinical Specialist Vocational School of Traditional Chinese Medicine Encephalopathy, Xiangya Hospital, Central South University,Changsha,China
2. Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University,Changsha,China
3. Department of Neurosurgery, Xiangya Hospital, Central South University,Changsha,China
4. Institute of Clinical Pharmacology, Central South University,Changsha,China
- Author bio：
- Funds：Supported by the Fund for Key Laboratory of Traditional Chinese Medicine Gan of State Administration of Traditional Chinese Medicine, the Major Research Plan of the National Natural Science Foundation of China (No. 90409010), and was partly supported by the Huge Project to Boost Chinese Drug Development (No. 2009ZX09304-003)
DOI：10.1007/s11655-012-1053-7
中图分类号：纸质出版日期：2012，
网络出版日期：2012-8-31，
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Wang, Y., Huang, X., Liang, Qh. et al. A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector., Chin. J. Integr. Med. 18, 690–698 (2012). https://doi.org/10.1007/s11655-012-1053-7
Yang Wang, Xi Huang, Qing-hua Liang, et al. A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector[J]. Chinese Journal of Integrative Medicine, 2012,18(9):690-698.
Wang, Y., Huang, X., Liang, Qh. et al. A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector., Chin. J. Integr. Med. 18, 690–698 (2012). https://doi.org/10.1007/s11655-012-1053-7 DOI：
Yang Wang, Xi Huang, Qing-hua Liang, et al. A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector[J]. Chinese Journal of Integrative Medicine, 2012,18(9):690-698. DOI： 10.1007/s11655-012-1053-7.
摘要
To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Anthraquinones in water extract of rhubarb (WER)
in cerebrospinal fluid (CSF) of patients with traumatic brain injury (TBI) and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase (SOD) activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Anthraquinones in WER were aloe-emodin
rhein
emodin
chrysophanol
and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy
the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
Abstract
To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Anthraquinones in water extract of rhubarb (WER)
in cerebrospinal fluid (CSF) of patients with traumatic brain injury (TBI) and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase (SOD) activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Anthraquinones in WER were aloe-emodin
rhein
emodin
chrysophanol
and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy
the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
关键词
quality controlabsorbed bioactive compoundsrhubarbultra performance liquid chromatographyCraniocerebral Injuryanthraquinones
Keywords
quality controlabsorbed bioactive compoundsrhubarbultra performance liquid chromatographyCraniocerebral Injuryanthraquinones
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