In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation

Ming-yu Zhang; Hui Qiang; Hua-qing Yang; Xiao-qian Dang; Kun-zheng Wang

doi:10.1007/s11655-012-1056-4

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In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation

OriginalPaper | Updated：2021-08-27

- In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation
- In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation
- 中国结合医学杂志（英文版） 2012年18卷第4期页码：276-282
- Affiliations：
  
  1. Department of Sport Injury, the Affiliated Red Cross Hospital of Medical College of Xi’an Jiaotong University,Xi’an,China
  2. Department of Orthopedics, Shaanxi Provincial People’s Hospital,Xi’an,China
  3. Department of Orthopedics, the Second Hospital of Tsinghua University,Beijing,China
  4. Department of Orthopedics, the Second Affiliated Hospital of Medical College of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the Doctoral Fund of Ministry of Education of China (No. 20070698083)
- DOI：10.1007/s11655-012-1056-4
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-3-30，
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Zhang, My., Qiang, H., Yang, Hq. et al. , In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation., Chin. J. Integr. Med. 18, 276–282 (2012). https://doi.org/10.1007/s11655-012-1056-4

Ming-yu Zhang, Hui Qiang, Hua-qing Yang, et al. In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation[J]. Chinese Journal of Integrative Medicine, 2012,18(4):276-282.
Zhang, My., Qiang, H., Yang, Hq. et al. , In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation., Chin. J. Integr. Med. 18, 276–282 (2012). https://doi.org/10.1007/s11655-012-1056-4 DOI：

Ming-yu Zhang, Hui Qiang, Hua-qing Yang, et al. In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation[J]. Chinese Journal of Integrative Medicine, 2012,18(4):276-282. DOI： 10.1007/s11655-012-1056-4.

摘要

To assess the effect of puerarin

a natural flavonoid found in Chinese Pueraria Lobata (Wild.) Ohwi

on promotion of new bone formation. Osteoblasts isolated from calvarial of newborn rats were cultured in vitro in the presence of puerarin at various concentrations. The viability of osteoblasts and alkaline phosphotase activity and mineral node formation were determined. In addition

osteoblasts seeded in the β-tricaclium phosphate scalfolds as bone substitute were implanted in rat dorsal muscles. Half -of the recipient rats received intramuscular injection of puerarin at 10 mg/(kg·d) for 7 days. Osteogenesis was analyzed by examining the histology after 4 weeks of implantation. The viability of osteoblasts treated with puerarin at either 40 or 80 μmol/L was significantly higher than that of the control (P<0.05 and P<0.01

respectively). Alkaline phosphatase and mineral modules were significantly increased in osteoblasts cultured with puerarin at 40 or 80 mol/L when compared with that of the untreated cells. The puerarin-treated rats had a higher rate of bone formation in the osteoblast implants than the control rats (6.35% vs. 1.32%

respectively

P<0.05). Puerarin was able to affect osteoblast proliferation and differentiation

and promote the new bone formation in osteoblast implants.

Abstract

To assess the effect of puerarin

a natural flavonoid found in Chinese Pueraria Lobata (Wild.) Ohwi

respectively

P<0.05). Puerarin was able to affect osteoblast proliferation and differentiation

and promote the new bone formation in osteoblast implants.

关键词

Puerarinosteoblastβ-tricalcium phosphatenew bone formation

Keywords

Puerarinosteoblastβ-tricalcium phosphatenew bone formation

references

Hirokazu K, Takaaki T, Masaaki C, Takahiro K. Repair of segmental bone defects in rabbit tibiae using a complex of b-tricalcium phosphate, type I collagen, and fibroblast growth factor-2. Biomaterials 2006;27:5118–5126.

Kneser U, Schaefer DJ, Polykandriotis E, Horch RE. Tissue engineering of bone: the reconstructive surgeon’s point of view. J Cell Mol Med 2006;10:7–19.

Smith LA, Liu XH, Ma PX. Nano-fibrous scaffolds for tissue engineering. Soft Matter 2008;4:2144–2149.

Liang G, Yang Y, Oh S, Ong JL, Zheng C, Ran J, et al. Ectopic osteoinduction and early degradation of recombinant human bone morphogenetic protein-2-loaded porous beta-tricalcium phosphate in mice. Biomaterials 2005;26:4265–4271.

Langer R, Vacanti JP. Tissue engineering. Science 1993;260:920–926.

Eppley BL, Pietrzak WS, Blanton MW. Allograft and alloplastic bone substitutes: a review of science and technology for the craniomaxillofacial surgeon. J Craniofac Surg 2005;16:981–989.

Kneser U, Schaefer DJ, Polykandriotis E, Horch RE. Tissue engineering of bone: the reconstructive surgeon’s point of view. J Cell Mol Med 2006;10:7–19.

Anderson JB, Garner SC. Phytoestrogens and bone. Baillieres Clin Endocrinol Metab 1998;12:543–557.

Wu HQ, Guo HN, Wang HQ, Chang MZ, Zhang GL, Zhao YX. Protective effect and mechanism of puerarin on learning-memory disorder after global cerebral ischemiareperfusion injury in rats. Chin J Integr Med 2009;15:54–59.

Wang XX, Wu J, Chiba H, Umegaket K, Yamada K, Ishimi Y. Puerariae radix prevents bone loss in ovariectomized mice. J Bone Miner Metab 2003;21:268–275.

Blarir, HC, Jordan SE, Peterson TG, Stephenal B. Variable effects of ty-rosine kinase inhibitor on avian osteoclastic activity and reduction of bone loss in ovariectiomized rats. J Cell Biochem 1996;61:629–637.

Heidi D, Natasj VV, Erna D, Maeyer Ronald V, Etienne S, Leo DR, et al. Isolation, proliferation and differentiation of osteoblastic cells to study cell/biomaterial interactions: comparison of different isolation techniques and source. Biomaterials 2004;25:757–768.

Yuan J, Cui L, Zhang WJ, Liu W, Cao Y. Repair of canine mandibular bone defects with bone marrow stromal cells and porous beta-tricalcium phosphate. Biomaterials 2007;28:1005–1013.

Zhou Q, Fu T. Effect of puerarin on the healing osteoporotic fracture in ovariectomized rats. Chin J Clin Rehabilit 2006;10:45–47.

Habibovic P, Yuan H, van der Valk CM, Meijer G, van Blitterswijk CA, de Groot K. 3D microenvironment as essential element for osteoinduction by biomaterials. Biomaterials 2005;26:3565–3575.

Dang ZC, Audinot V, Papapoulos SE, Boutin JA, Löwik CWGM. Peroxisome proliferator-activated receptor g (PPARg) as a molecular target for the soy phytoestrogen genistein. J Biol Chem 2003;278:962–967.

Dang ZC, Löwik CW. The balance between concurrent activation of ERs and PPARs determines daidzeininduced osteogenesis and adipogenesis. J Bone Miner Res 2004;19:853–861.

Viereck V, Gründker C, Blaschke S, Siggelkow H, Emons G, Hofbauer LC. Phytoestrogen genistein stimulates the production of osteoprotegerin by human trabecular osteoblasts. J Cell Biochem 2002;84:725–735.

Notoya M, Tsukamoto Y, Nishimura H, Woo JT, Nagai K, Lee IS, et al. Quercetin, a flavonoid, inhibits the proliferation, differentiation, and mineralization of osteoblasts in vitro. Eur J Pharmacol 2004;485:89–96.

Garcia T, Roman S, Jackson A, Thellhaber J, Connolly T, Spinella-Jaegle S, et al. Behavior of osteoblast, adipocyte, and myoblast markers in genome-wide expression analysis of mouse calvaria primary osteoblasts in vitro. Bone 2002;31:205–211.

Whyte MP. Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocr Rev 1994;15:439–461.

Fedarko NS, Bianco P, Vetter U, Gehron Robey P. Human bone cell enzyme expression and cellular heterogeneity: correlation of alkaline phosphatase enzyme activity with cell cycle. J Cell Physiol 1990;144:115–121.

Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA. Mineralization and expression of matrix proteins during in vivo bone development. Calcif Tissue Int 1998;62:74–82.

Maenoa S, Nikia Y, Matsumotoa H, Morioka H, Yatabe T, Funayama A, et al. The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials 2005;26:4847–4855.

Xu SL, Li DC, Xie YZ, Lu JX, Dai KR. The growth of stem cells within β-TCP scaffolds in a fluid-dynamic environment. Mater Sci Engin 2008;C28:164–170.

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