Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head

Wei-heng Chen; Xiang-ying Kong; Rong Wan; Chun-sheng Xiao; Li Li; Zhi-yao Wang; Na Lin; He-ming Wang

doi:10.1007/s11655-012-1086-y

Your Location：

Home >

Browse articles >

Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head

OriginalPaper | Updated：2021-08-27

- Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head
- Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head
- 中国结合医学杂志（英文版） 2012年18卷第5期页码：378-384
- Affiliations：
  
  1. Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing,China
  3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Foundation of China (No. 81173417 and 30472131)
- DOI：10.1007/s11655-012-1086-y
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-5-2，
Scan for full text
Chen, Wh., Kong, Xy., Wan, R. et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head., Chin. J. Integr. Med. 18, 378–384 (2012). https://doi.org/10.1007/s11655-012-1086-y

Wei-heng Chen, Xiang-ying Kong, Rong Wan, et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head[J]. Chinese Journal of Integrative Medicine, 2012,18(5):378-384.
Chen, Wh., Kong, Xy., Wan, R. et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head., Chin. J. Integr. Med. 18, 378–384 (2012). https://doi.org/10.1007/s11655-012-1086-y DOI：

Wei-heng Chen, Xiang-ying Kong, Rong Wan, et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head[J]. Chinese Journal of Integrative Medicine, 2012,18(5):378-384. DOI： 10.1007/s11655-012-1086-y.

摘要

To study the mechanism of Huogu I Formula (活骨I方) in treating osteonecrosis of femoral head. Forty-eight healthy female Leghorn chickens were randomly divided into control group

model group and Huogu I group

and each group consisted of 16 chickens. At the meantime of model establishment

chickens of the Huogu I group were administrated with decoction

while the model and control group with distilled water by gavage. At the 8th and 16th week after medication

blood samples were obtained for blood lipid detection while both sides of femoral head were harvested for the rest of examinations. Specifically

expressions of bone morphogenetic protein-2 (BMP2)

transforming growth factor beta1 (TGFβ1)

Smad4 and Smad7 were evaluated by immunohistochemistry

while expression of osteoprotegerin/receptor activator of nuclear factor kappaB ligand (OPG/RANKL) mRNA was detected by in situ hybridization. Compared with the control group

serum levels of total cholesterol (TC)

triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in the model group rose significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the model group dropped prominently. Positive cell counting of Smad7 and RANKL increased dramatically. In contrast with the model group

levels of TC

TG and LDL-C in Huogu I group reduced significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the Huogu I group increased prominently. Indices of Smad7 and RANKL both decreased significantly. Especially at the 8th week

these variations were more significant. Huogu I Formula is effective in promoting repair of necrotic femoral head by regulating the expressions of BMP2

TGFβ1

Smads and OPG/RANKL of osteoclast in femoral head.

Abstract

To study the mechanism of Huogu I Formula (活骨I方) in treating osteonecrosis of femoral head. Forty-eight healthy female Leghorn chickens were randomly divided into control group

model group and Huogu I group

and each group consisted of 16 chickens. At the meantime of model establishment

chickens of the Huogu I group were administrated with decoction

while the model and control group with distilled water by gavage. At the 8th and 16th week after medication

blood samples were obtained for blood lipid detection while both sides of femoral head were harvested for the rest of examinations. Specifically

expressions of bone morphogenetic protein-2 (BMP2)

transforming growth factor beta1 (TGFβ1)

Smad4 and Smad7 were evaluated by immunohistochemistry

while expression of osteoprotegerin/receptor activator of nuclear factor kappaB ligand (OPG/RANKL) mRNA was detected by in situ hybridization. Compared with the control group

serum levels of total cholesterol (TC)

triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in the model group rose significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the model group dropped prominently. Positive cell counting of Smad7 and RANKL increased dramatically. In contrast with the model group

levels of TC

TG and LDL-C in Huogu I group reduced significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the Huogu I group increased prominently. Indices of Smad7 and RANKL both decreased significantly. Especially at the 8th week

these variations were more significant. Huogu I Formula is effective in promoting repair of necrotic femoral head by regulating the expressions of BMP2

TGFβ1

Smads and OPG/RANKL of osteoclast in femoral head.

关键词

osteonecrosis of femoral headbone morphogenetic protein-2transforming growth factor beta1osteoprotegerinreceptor activator of nuclear factor kappaBSmads

Keywords

osteonecrosis of femoral headbone morphogenetic protein-2transforming growth factor beta1osteoprotegerinreceptor activator of nuclear factor kappaBSmads

references

Chen WH, Xu R, Ou TW, Ma LL, Zhou Y, He HJ. A preliminary study on prevention of osteonecrosis of femoral head by Chinese medicine. Beijing J Tradit Chin Med (Chin) 2008;27:761–763.

Wang XS, Kang WC, Xu ZH. Methods for establishing animal model of steroid-induced osteonecrosis of femoral head. Chin J Exp Surg (Chin) 1993;10:136–138.

Wang KZ, Mao LZ, Hu CG, He XJ, Chen JC, Yang Z. An experimental study on pathogenesis of steroid-induced avascular necrosis of femoral head. Chin J Surg (Chin) 1994;32:515–516.

Cárcamo-Orive I, Gaztelumendi A, Delgado J, Tejados N, Dorronsoro A, Fernández-Rueda J, et al. Regulation of human bone marrow stromal cell proliferation and differentiation capacity by glucocorticoid receptor and AP-1 crosstalk. J Bone Miner Res 2010;25:2115–2125.

Yun SI, Yoon HY, Jeong SY, Chung YS. Glucocorticoid induces apoptosis of osteoblast cells through the activation of glycogen synthase kinase 3beta. J Bone Miner Metab 2009;27:140–148.

Wang FS, Ko JY, Yeh DW, Ke HC, Wu HL. Modulation of Dickkopf-1 attenuates glucocorticoid induction of osteoblast apoptosis, adipocytic differentiation, and bone mass loss. Endocrinology 2008;149:1793–1801.

Song JN. A study on phlegm and correlation between phlegm and stasis from the perspective of biochemistry. Chin J Basic Med Tradit Chin Med (Chin) 2000;6:40–42.

Chen WH. The theoretical basis of simultaneous treatment of phlegm and blood stasis. Jiangsu J Tradit Chin Med (Chin) 2008;40(5):3–5.

Chen WH, Liu DB, Zhang Q, Sun G, Zhou W, Wang S. A study on syndrome characteristics and optimization of therapeutic regimen of osteonecrosis of femoral head developed after SARS. Part I. Chin Med Modern Distance Educ China (Chin) 2006;4(10):55–58.

He HJ, Chen WH, Wang RT, Liu DB, Zhou Y, Xie B. A study on evaluation of quality of life in patients with osteonecrosis of femoral head and their management by Chinese medicine through multiple ways. Chin J Tradit Med Traumatol Orthoped (Chin) 2010;18(10):13–15.

Vande-Berg BC, Malghem J, Lecouvet FE, Devoqelaer JP, Maldaque B, Houssiau FA. Fat conversion of femoral marrow in glucocorticoid-treated patients: a cross-sectional and longitudinal study with magnetic resonance imaging. Arthritis Rheum 1999;42:1405–1411

Han H, Wu LM, Yang WM, Wang MX, Dong T, Wang H. Effect of Shenxiongqin Injection on hemorheology and cerebral water content in rats with focal cerebral ischemia. Chin J Integr Med Cardio-Cerebrovasc Dis (Chin) 2010;8:79–81.

Wen L, Ye Y, Liu SL, Mei GQ. Effects of Huatanhuoxue Decoction and its decomposed recipes on blood lipid metabolism of experimental rats with hyperlipidemia. Chin J Manag Chin Med (Chin) 2007;15:130–132.

Tang CP, Jiang T, Guo J, Wei YP, Yan CY, Chen FC. A probe into the effect of Fufang Zhenzhu Tiaozhi Capsules on lipid metabolism in an atherosclerotic rabbit model. J Chin Med Mater (Chin) 2010;33:1285–1289.

Anderson DM, Maraskovsky E, Billingsley WL, Dougall WC, Tometsko ME, Roux ER, et al. A homologue of the TNF receptor and its ligand enhance T-cell growth and dendriticcell function. Nature 1997;390:175–177.

Lacey DL, Tan HL, Lu J, Kaufman S, Van G, Qiu W, et al. Osteoprotegerin ligand modulates murine osteoclast survival in vitro and in vivo. Am J Pathol 2000;157:435–437.

Teitelbaums L. Bone resorption by osteoclasts. Science 2000;289:1504–1508.

Chamoux E, Houde N, L’eriger K, Roux S. Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway. J Cell Physiol 2008;216:536–539.

Osawa H, Nakajima M, Kato H, Fukuchi M, Kuwano H. Prognostic value of the expression of Smad6 and Smad7, as inhibitory Smads of the TGF-beta superfamily, in esophageal squamous cell carcinoma. Anticancer Res 2004;24:3703–3707.

Yang LJ, Jin Y. Immunohistochemical observations on bone morphogenetic protein in normal and abnormal conditions. Clin Orthop 1990;257:249–253.

Wang JZ, Wang KZ, Shi ZB, Zhang MY. Osteoprotegerin mRNA/receptor activator of NF-κB ligand mRNA expressions in bone tissue of steroid-induced osteonecrosis of the femoral head. Chin J Rep Reconstr Surg (Chin) 2008;22:1161–1163.

Wang JJ, Luo WZ, Qi JX, Song HJ, Shagn Y, Wang ML. A study on effects of tetramethylpyrazine on expression of RANK/RANKL/OPG in synovial fluid of rheumatoid arthritis rat model. Chin J Gerontol (Chin) 2011;3:833–835.

Feng W, Fu W, Wei YY, Zhang WY, Wang J, Zhu YP. A research on effects of single Chinese herb on expression of osteogenesis-related genes. J Tradit Chin Orthop Traumatol (Chin) 2004;16(3):6–8.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Oxysophoridine suppresses the growth of hepatocellular carcinoma in mice: In Vivo and cDNA microarray studies

Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction

Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis