Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress

He-gu Luo; Jia-xu Chen; Qi Zhang; Guang-xin Yue; Jie Ding; Han-ting Zhang; Xian-zhong Yan; Xin Zhao; Zhen-zhi Meng

doi:10.1007/s11655-012-1092-0

Your Location：

Home >

Browse articles >

Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress

OriginalPaper | Updated：2021-08-27

- Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress
- Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress
- 中国结合医学杂志（英文版） 2013年19卷第8期页码：610-615
- Affiliations：
  
  1. School of Pre-clinical Medicine, Beijing University of Chinese Medicine,Beijing,China
  2. National Center of Biomedical Analysis,Beijing,China
  3. Department of Behavioral Medicine and Psychiatry, West Virginia University Health Sciences Center, Morgantown,USA
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30672578), China National Funds for Distinguished Young Scientists (No. 30825046), and Program for Innovative Research Team in Beijing University of Chinese Medicine (No. 2011CXTD-07)
- DOI：10.1007/s11655-012-1092-0
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2012-5-19，
Scan for full text
Luo, Hg., Chen, Jx., Zhang, Q. et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress., Chin. J. Integr. Med. 19, 610–615 (2013). https://doi.org/10.1007/s11655-012-1092-0

He-gu Luo, Jia-xu Chen, Qi Zhang, et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress[J]. Chinese Journal of Integrative Medicine, 2013,19(8):610-615.
Luo, Hg., Chen, Jx., Zhang, Q. et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress., Chin. J. Integr. Med. 19, 610–615 (2013). https://doi.org/10.1007/s11655-012-1092-0 DOI：

He-gu Luo, Jia-xu Chen, Qi Zhang, et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress[J]. Chinese Journal of Integrative Medicine, 2013,19(8):610-615. DOI： 10.1007/s11655-012-1092-0.

摘要

To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress (CIS) taking Xiaoyao Powder (逍遥散

XYP) and its modified prescription version

which lacks the volatile oils extracted from Herba Menthae. Twenty-four experimental male SD rats were randomly divided into 4 groups of 6 rats each: control

model

XYP-1 (containing volatile oils from Herba Menthae)

and XYP-2 (lacking volatile oils). All rats except control group rats were subjected to CIS 3 h per day for 21 consecutive days. Groups XYP-1 and XYP-2 were given the extracted XYS with or without volatile oils (3.854 g/kg; suspended in distilled water) via gavage 1 h before CIS each day for 21 days. Rats were anesthetized using intraperitoneal injection of pentobarbital sodium (40 mg/kg) on the 22nd day. Observations were made using a Varian INOVA 600 MHz NMR spectrometer at 27 °C. Carr-Purcell-Meiboom-Gill (CPMG) and longitudinal eddy-delay (LED) were applied

resulting in spectra showing only the signals from micro- and macro-metabolites. Compared to controls

rats subjected to CIS showed increased levels of plasma metabolites

such as acetic acid

choline

N-glycoprotein (NAC)

saturated fatty acid

and blood sugars. Levels of low-density lipoprotein (LDL)

very low-density lipoprotein (VLDL)

and unsaturated fatty acids were decreased. The biochemical effects of XYS were characterized by elevated levels of VLDL

LDL

threonine

methionine

and glutamic acid in plasma. Some common and characteristic metabolites on the anti-CIS of XYP and its modified prescription were obtained. The metabolomics technology is a valuable tool and may be used to identify the specific metabolites and potential biomarkers of therapeutic effect of Chinese medicinal prescriptions.

Abstract

To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress (CIS) taking Xiaoyao Powder (逍遥散

XYP) and its modified prescription version

which lacks the volatile oils extracted from Herba Menthae. Twenty-four experimental male SD rats were randomly divided into 4 groups of 6 rats each: control

model

XYP-1 (containing volatile oils from Herba Menthae)

resulting in spectra showing only the signals from micro- and macro-metabolites. Compared to controls

rats subjected to CIS showed increased levels of plasma metabolites

such as acetic acid

choline

N-glycoprotein (NAC)

saturated fatty acid

and blood sugars. Levels of low-density lipoprotein (LDL)

very low-density lipoprotein (VLDL)

and unsaturated fatty acids were decreased. The biochemical effects of XYS were characterized by elevated levels of VLDL

LDL

threonine

methionine

关键词

metabolomicschronic immobilization stressbiomarkerChinese MedicineXiaoyao Powder

Keywords

metabolomicschronic immobilization stressbiomarkerChinese MedicineXiaoyao Powder

references

Mazure CM. Does stress cause psychiatric illness? In: Spiegel D, ed. Progress in psychiatry. Washington D. C.: American Psychiatric Press; 1995:270–298.

Ricart-Jané DV, RodrÍguez-Sureda A, Benavides J, Peinado-Onsurb MD, López-Tejero, Llobera M. Immobilication stress alters intermediate metabolism and circulating lipoproteins in the rat. Metabolism 2002;51:925–931.

Vanitallie TB. Stress: a risk factor for serious illness. Metabolism 2002;51(6 Suppl. 1):40–45.

Brinkley DN, Rolland Y. Possible connections between stress, diabetes, obesity, hypertension and altered lipoprotein metabolism that may result in atherosclerosis. Clin Sci 1989;77:453–461.

Tang YT, Chen JX. Regulative effects of three TCM formulas on hypothalamus-pituitary-adrenal axis in the rats with chronic restrained stress. J Beijing Univ Tradit Chin Med (Chin) 2002;25:23–26.

Chen JX, Li W, Zhao X, Yang JX, Xu HY, Wang ZF, et al. Changes of mRNA expression of enkephalin and prodynorphin in hippocampus of rats with chronic immobilization stress. World J Gastroenterol 2004;10:2547–2549.

Chen JX, Li W, Zhao X, Yang JX. Effects of the Chinese traditional prescription Xiaoyaosan decoction on chronic immobilization stress-induced changes in behavior and brain BDNF, TrkB, and NT-3 in rats. Cell Mol Neurobiol 2008;5:745–755.

Chen JX, Tang YT, Yang JX. Changes of glucocorticoid receptor and levels of CRF mRNA, POMC mRNA in brain of chronic immobilization stress rats. Cell Mol Neurobiol 2008;28:237–234.

Teague CR, Dhabhar FS, Barton RH, Beckwith-Hall B, Powell J, Cobain M, et al. Metabonomic studies on the physiological effects of acute and chronic psychological stress in Sprague-Dawley rats. J Proteome Res 2007;6:2080–2093.

Nicholson JK, Lindon JC, Holmes E. “Metabonomics”: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data. Xenobiotica 1999;29:1181–1189.

Nicholson JK, Wilson LD. High resolution nuclear magnetic resonance spectroscopy of biological samples as an aid to drug development. Prog Drug Res 1987;31:427–479.

Nicholson JK, Wilson ID. Opinion: understanding “global” systems biology: metabonomics and the continuum of metabolism. Nat Rev Drug Discov 2003;2:668–676.

Wang Y, Holmes E, Tang H, Lindon JC, Sprenger N, Turini ME, et al. Experimental metabonomic model of dietary variation and stress interactions. J Proteome Res 2006;5:1535–1542.

Waters NJ, Waterfield CJ, Farrant RD, Holmes E, Nicholson JK. Metabonomic deconvolution of embedded toxicity: application to thioacetamide hepato- and nephrotoxicity. Chem Res Toxicol 2005;4:639–654.

Lindon JC, Nicholson JK, Holmes E, Everett JR. Metabonomics: metabolic processes studied by NMR spectroscopy of biofluids. Concepts Magn Reson 2000;12:289–320.

Luo HG, Chen JX, Yue GX, Ding J, Yan XZ. Metabonomic study on the regulatory effect of Xiaoyao Powder on chronic immobilization stressed rats. Chin J Integr Tradit West Med (Chin) 2008;12:1112–1116.

Yue GX, Wang ZF, Zhang QL, Chen JX, Zhao X, Yue LF, et al. Changes on AMPA receptors and levels of related proteins’ mRNA in brain of chronic immobilization stress rats and regulative effects on them. J Chin Integr Med (Chin) 2007;12:1110–1115.

Shi HL, Cheng YY, Li ST, Wang DL, Yu ZJ, Geng ZH, et al. Effects of chronic restraint stress on intestinal barrier function of rats. Chin J Behav Med Sci (Chin) 2003;3:251–253.

Lv ZP. Changes of lipid peroxidation in rats of stagnation of Liver-qi and effect of Xiaoyao Power. J Shandong Coll Tradit Chin Med (Chin) 1995;3:199.

Lu ZP, Liu CC. Changes of TXA2, PGI2 in rats of stagnation of Liver-qi and liver microcirculatory and effect of Xiaoyao Power. Chin J Microcircul (Chin) 2000;3:160.

FULL TEXT LINK

More>

浏览量

1119

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients

Objective tongue inspection on 142 liver cancer patients with damp-heat syndrome

Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats