Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf

Jing-shang Wang; Hui-jun Yin; Chun-Yu Guo; Ye Huang; Cheng-dong Xia; Qian Liu

doi:10.1007/s11655-012-1093-z

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Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf

OriginalPaper | Updated：2021-08-27

- Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf
- Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf
- 中国结合医学杂志（英文版） 2013年19卷第3期页码：217-222
- Affiliations：
  
  Departmten of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Science,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81041038)
- DOI：10.1007/s11655-012-1093-z
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2012-5-19，
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Wang, Js., Yin, Hj., Guo, CY. et al. Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf., Chin. J. Integr. Med. 19, 217–222 (2013). https://doi.org/10.1007/s11655-012-1093-z

Jing-shang Wang, Hui-jun Yin, Chun-Yu Guo, et al. Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf[J]. Chinese Journal of Integrative Medicine, 2013,19(3):217-222.
Wang, Js., Yin, Hj., Guo, CY. et al. Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf., Chin. J. Integr. Med. 19, 217–222 (2013). https://doi.org/10.1007/s11655-012-1093-z DOI：

Jing-shang Wang, Hui-jun Yin, Chun-Yu Guo, et al. Influence of high blood glucose fluctuation on endothelial function of type 2 diabetes mellitus rats and effects of Panax Quinquefolius Saponin of stem and leaf[J]. Chinese Journal of Integrative Medicine, 2013,19(3):217-222. DOI： 10.1007/s11655-012-1093-z.

摘要

To observe the influence of high blood glucose fluctuation on the endothelial function of type 2 diabetes mellitus (T2DM) rats and the effects of Panax Quinquefolius Saponin (PQS) of stem and leaf. The T2DM model was induced by intraperitoneal injection of a small dose of streptozotocin (STZ

35 mg/kg) plus high fat and high caloric laboratory chow. Then

diabetic rats were divided into steady high blood glucose (SHG) group and fluctuant high blood glucose (FHG) group according to fasting blood glucose coefficient of variation (FBG-CV)

and then

the FHG group rats were divided into 4 groups according to the level of FBG-CV and fasting blood glucose: PQS 30 mg/(kg·d) group

PQS 60 mg/(kg·d) group

metformin hydrochloride control (MHC) group

and FHG control group

10 in each group. Meanwhile

10 rats without any treatment were used as normal control (NOR) group. Eight weeks later

the aortic arteries histology

plasma hepatocyte growth factor (HGF)

and serum nitric oxide (NO)

endothelin-1 (ET-1)

tumor necrosis factor α (TNF-α)

and soluble intercellular adhesion molecule 1 (sICAM-1) were measured. In comparison with the NOR group

the level of plasma HGF and serum NO

ET-1 and TNF-α

and sICAM-1 in SHG and FHG control groups were all significantly increased (P<0.01); in comparison with the SHG group

plasma HGF and serum NO

ET-1

TNF-α

and sICAM-1 in FHG group were all significantly increased further (P<0.01 or P<0.05); meanwhile

in comparison with the FHG control group

the level of plasma HGF and serum NO

ET-1

TNF-α

and sICAM-1 in PQS and MHC groups were all decreased significantly (P<0.01). However

comparison of the aortic arteries histology among groups showed no significant differences either before or after treatment. Blood glucose fluctuation could facilitate the development of vascular endothelial dysfunction in T2DM rats

while PQS could improve the endothelial function of T2DM rats with high blood glucose fluctuation

which may be related to its effects of relieving vessel stress

decreasing vasoconstrictor ET-1 production

preventing compensated increase of NO

and reducing inflammatory reaction.

Abstract

35 mg/kg) plus high fat and high caloric laboratory chow. Then

diabetic rats were divided into steady high blood glucose (SHG) group and fluctuant high blood glucose (FHG) group according to fasting blood glucose coefficient of variation (FBG-CV)

and then

the FHG group rats were divided into 4 groups according to the level of FBG-CV and fasting blood glucose: PQS 30 mg/(kg·d) group

PQS 60 mg/(kg·d) group

metformin hydrochloride control (MHC) group

and FHG control group

10 in each group. Meanwhile

10 rats without any treatment were used as normal control (NOR) group. Eight weeks later

the aortic arteries histology

plasma hepatocyte growth factor (HGF)

and serum nitric oxide (NO)

endothelin-1 (ET-1)

tumor necrosis factor α (TNF-α)

and soluble intercellular adhesion molecule 1 (sICAM-1) were measured. In comparison with the NOR group

the level of plasma HGF and serum NO

ET-1 and TNF-α

and sICAM-1 in SHG and FHG control groups were all significantly increased (P<0.01); in comparison with the SHG group

plasma HGF and serum NO

ET-1

TNF-α

and sICAM-1 in FHG group were all significantly increased further (P<0.01 or P<0.05); meanwhile

in comparison with the FHG control group

the level of plasma HGF and serum NO

ET-1

TNF-α

and sICAM-1 in PQS and MHC groups were all decreased significantly (P<0.01). However

while PQS could improve the endothelial function of T2DM rats with high blood glucose fluctuation

which may be related to its effects of relieving vessel stress

decreasing vasoconstrictor ET-1 production

preventing compensated increase of NO

and reducing inflammatory reaction.

关键词

high blood glucose fluctuationendothelial functiontype 2 diabetes mellitusAmerican Ginseng

Keywords

high blood glucose fluctuationendothelial functiontype 2 diabetes mellitusAmerican Ginseng

references

Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive Summary of The third report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA 2001;285:2486–2497.

Guerci B, Kearney-Schwartz A, Böhme P, Zannad F, Drouin P. Endothelial dysfunction and type 2 diabetes. Part 1: physiology and methods for exploring the endothelial function. Diabetes Metab 2001;27:425–434.

Mazzone T, Chait A, Plutzky J. Cardiovascular disease risk in type 2 diabetes mellitus: insights from mechanistic studies. Lancet 2008;371:1800–1809.

Nordwall M, Arnqvist HJ, Bojestig M, Ludvigsson J. Good glycemic control remains crucial in prevention of late diabetic complications—the Linkoping Diabetes Complications Study. Pediatr Diabetes 2009;10:168–176.

Bonora E, Muggeo M. Postprandial blood glucose as a risk factor for cardiovascular disease in type II diabetes: the epidemiological evidence. Diabetologia 2001;44:2107–2114.

Hirsch IB, Brownlee M. ShouId minimal blood-variability become the gold standard of glycemic control? J Diabetes Complic 2005;19:178–181.

Monnier L, Colette C. Glycemic variability: should we and can we prevent it? Diabetes Care 2008;31(Suppl2):S150–S154.

O’Sullivan EP, Dinneen SF. Benefits of early intensive glucose control to prevent diabetes complications were sustained for up to 10 years. Evid Based Med 2009;14:9–10.

Yin HJ, Zhang Y, Jiang YR, Liu Y, Luo L, Shi DZ. Effects of panax quinquefolium saponin (PQS) on blood lipid metabolism of alloxan diabetic rats. Chin J Integr Med Cardio/Cerebrovasc Dis (Chin) 2004;2:647–648.

Yin HJ, Zhang Y, Shi DZ, Chen KJ, Yang LH, Bai GR. Effects of Panax Quinquefolium Saponin (PQS) on glucose transportation, GLUT-4 translocation and CAP mRNA expression of insulin resistance adipocytes. Chin Pharmacol Bull (Chin) 2007;23:1332–1337.

Zhang Y, Chen KJ, Yang LH, Bai GH, Yin HJ. Effects of panax quinquefolius saponin of stem and leaf on glucose-lipid metabolism and insulin signal transduction in insulin resistant model adipocytes. Chin J Integr Med 2010;30:748–751.

Fan BJ, Fei F, Zhao XZ. Effects of panax quinquefolium saponin (PQS) on the vascular endothelial function of the myocardial hypertrophied rats. Chin J Gerontol (Chin) 2009;29:811–812.

Muggeo M, Zoppini G, Bonora E, Brun E, Bonadonna RC, Moghetti P, et al. Fasting plasma glucose variability predicts 10-year survival of type 2 diabetes patients: the Verona Diabetes Study. Diabetes Care 2000;23:45–50.

Piconi L, Quagliaro L, Assaloni R, Da Ros R, Maier A, Zuodar G, et al. Constant and intermittent high glucose enhances endothelial cell apoptosis through mitochondrial superoxide overproduction. Diabetes Metab Res Rev 2006;22:198–203.

Azuma K, Kawamori R, Toyofuku Y, Kitahara Y, Sato F, Shimizu T, et al. Repetitive fluctuations in blood glucose enhance monocyte adhesion to the endothelium of rat thoracic aorta. Arterioscler Thromb Vasc Biol 2006;26:2275–2280.

Kilpatrick ES. Arguments for and against the role of glucose variability in the development of diabetes complications. J Diabetes Sci Technol 2009;3:649–655.

Risso A, Mercuri F, Quagliaro L, Damante G, Ceriello A. Intermittent high glucose enhances apoptosis in human umbilical vein endothelial cells in culture. Am J Physio 2001;281:E924–E930.

Horvath EM, Benko R, Kiss L, Muranyi M, Pek T, Fekete K, et al. Rapid “glycaemic swings” induce nitrosative stress, activate poly (ADP-ribose) polymerase and impair endothelial function in a rat model of diabetes mellitus. Diabetologia 2009;52:952–961.

Mita T, Otsuka A, Azuma K, Uchida T, Ogihara T, Fujitani Y, et al. Swings in blood glucose levels accelerate atherogenesis in apolipoprotein E-deficient mice. Biochem Biophys Res Commun 2007;358:679–985.

Tu Q, Weng YJ, Tong Z, Ye XY. Establishment of blood glucose fluctuation model on diabetes mice and its damage to viscera. J Fudan Univ (Nat Sci) 2008;47:647–651.

Ceriello A, Esposito K, Piconi L, Ihnat MA, Thorpe JE, Testa R, et al. Oscillating glucose is more deleterious to endothelial function and oxidative stress than mean glucose in normal and type 2 diabetic patients. Diabetes 2008;57:1349–1354.

Quagliaro L, Piconi L, Assaloni R, Da Ros R, Maier A, Zuodar G, et al. Intermittent high glucose enhances ICAM-1, VCAM-1 and E-selectin expression in human umbilical vein endothelial cells in culture: the distinct role of protein kinase C and mitochondrial superoxide production. Atherosclerosis 2005;183:259–267.

Piconi L, Quagliaro L, Da Ros R, Assaloni R, Giugliano D, Esposito K, et al. Intermittent high glucose enhances ICAM-1, VCAM-1, E-selectin and interleukin-6 expression in human umbilical endothelial cells in culture: the role of poly (ADPribose) polymerase. J Thromb Haemost 2004;2:1453–1459.

Piconi L, Corgnali M, Da Ros R, Assaloni R, Piliego T, Ceriello A. The protective effect of rosuvastatin in human umbilical endothelial cells exposed to constant or intermittent high glucose. J Diabetes Complic 2008;22:38–45.

Zhang Y, ed. Effect of Panax Quinquefolius saponin on insulin sensitivity in patients of coronary heart disease and its mechanism. Beijing: China Academy of Chinese Medical Sciences; 2006.

Chinese Diabetes Society. The 2007 guideline of Chinese type 2 diabetes prevents and controls. Nat Med J China 2008;88:1227–1245.

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