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Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture
Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture
- 中国结合医学杂志（英文版） 2013年19卷第8期 页码：573-581
- Affiliations：
1. State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University,Tianjin,China
2. School of Fundamental Medical Sciences, Guangzhou University of Chinese Medicine,Guangzhou,China
3. School of Nursing, Guangzhou University of Chinese Medicine,Guangzhou,China
4. Institute of Cardiovascular Diseases, Pingjin Hospital, Medical College of Armed Police Forces,Tianjin,China
- Author bio：
- Funds：These authors contributed equally to this work;Supported by the National Natural Science Foundation of China (No. 30500645, 30570471, 30725030) and National Key Project for Basic Research of China (No. 2011CB964903 and 2005CB523502)
DOI：10.1007/s11655-012-1101-3
中图分类号：纸质出版日期：2013，
网络出版日期：2012-8-18，
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Zhang, J., Jia, Xh., Xu, Zw. et al. Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture., Chin. J. Integr. Med. 19, 573–581 (2013). https://doi.org/10.1007/s11655-012-1101-3
Jin Zhang, Xiao-hua Jia, Zhi-wei Xu, et al. Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture[J]. Chinese Journal of Integrative Medicine, 2013,19(8):573-581.
Zhang, J., Jia, Xh., Xu, Zw. et al. Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture., Chin. J. Integr. Med. 19, 573–581 (2013). https://doi.org/10.1007/s11655-012-1101-3 DOI：
Jin Zhang, Xiao-hua Jia, Zhi-wei Xu, et al. Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture[J]. Chinese Journal of Integrative Medicine, 2013,19(8):573-581. DOI： 10.1007/s11655-012-1101-3.
摘要
To investigate whether electroacupuncture (EA) can promote cell survival and enhance heart function of mesenchymal stem cells (MSCs) therapy. MSCs were isolated from bone marrow and expanded in Minimum Essential Medium Alpha (α-MEM). MI was induced in 72 Sprague-Dawley (S-D) rats by ligation of the left anterior descending coronary artery (LAD) for 30 min and reperfusion. MI rats randomly received injection of 1×106 DiI-labeled MSCs alone (n =24
MSC group)
or plus electroacupuncture (EA) at Neiguan (PC6
n=24
EA+MSC group)
or saline (n =24
saline group). EA treatment was performed for 4 days. Another 24 rats were subjected to chest-open surgery without LAD occlusion and treatment (sham group). Three time points
4
14 and 28 days (n =8 for each group) were included in this study. The survival of transplanted MSCs and the protective gene expression were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot at day 4 and 14. Left ventricular remodeling
cardiac function
infarction area
fibrosis and capillary density were analyzed at day 28. EA can enhance MSC survival (2.6-fold up) at day 4. Big capillary density was 53% higher in EA+MSC treated group than MSC alone group. Furthermore
the rats treated by EA reduced the fibrosis and had 36% smaller infarct size comparing to MSC alone. EA also attenuated left ventricular remodeling and enhanced the functional recovery of infarcted hearts at week 4. EA at Neiguan acupoint can promote the stem cell survival and improve ischemic heart function. EA could become a useful approach in stem cell therapy for ischemia heart diseases.
Abstract
To investigate whether electroacupuncture (EA) can promote cell survival and enhance heart function of mesenchymal stem cells (MSCs) therapy. MSCs were isolated from bone marrow and expanded in Minimum Essential Medium Alpha (α-MEM). MI was induced in 72 Sprague-Dawley (S-D) rats by ligation of the left anterior descending coronary artery (LAD) for 30 min and reperfusion. MI rats randomly received injection of 1×106 DiI-labeled MSCs alone (n =24
MSC group)
or plus electroacupuncture (EA) at Neiguan (PC6
n=24
EA+MSC group)
or saline (n =24
saline group). EA treatment was performed for 4 days. Another 24 rats were subjected to chest-open surgery without LAD occlusion and treatment (sham group). Three time points
4
14 and 28 days (n =8 for each group) were included in this study. The survival of transplanted MSCs and the protective gene expression were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot at day 4 and 14. Left ventricular remodeling
cardiac function
infarction area
fibrosis and capillary density were analyzed at day 28. EA can enhance MSC survival (2.6-fold up) at day 4. Big capillary density was 53% higher in EA+MSC treated group than MSC alone group. Furthermore
the rats treated by EA reduced the fibrosis and had 36% smaller infarct size comparing to MSC alone. EA also attenuated left ventricular remodeling and enhanced the functional recovery of infarcted hearts at week 4. EA at Neiguan acupoint can promote the stem cell survival and improve ischemic heart function. EA could become a useful approach in stem cell therapy for ischemia heart diseases.
关键词
electroacupunctureNeiguan (PC6)mesenchymal stem cellMyocardial ischemiaapoptosis
Keywords
electroacupunctureNeiguan (PC6)mesenchymal stem cellMyocardial ischemiaapoptosis
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