Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection

Shao-xia Wang; Hong Guo; Li-min Hu; Ya-nan Liu; Yue-fei Wang; Li-yuan Kang; Xiu-mei Gao

doi:10.1007/s11655-012-1114-y

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Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection

OriginalPaper | Updated：2021-08-27

- Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection
- Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection
- 中国结合医学杂志（英文版） 2012年18卷第6期页码：437-444
- Affiliations：
  
  1. Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine,Tianjin,China
  2. Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine,Tianjin,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No.81173592, 81001654, 81102699), the Funds from the Ministry of Science and Technology of China (No.2009DFA31070, PCSIRT-IRT0973), and Tianjin Natural Science Fund (No.11JCZDJC21100, 08JCYBJC10800)
- DOI：10.1007/s11655-012-1114-y
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-7-22，
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Wang, Sx., Guo, H., Hu, Lm. et al. Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection., Chin. J. Integr. Med. 18, 437–444 (2012). https://doi.org/10.1007/s11655-012-1114-y

Shao-xia Wang, Hong Guo, Li-min Hu, et al. Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection[J]. Chinese Journal of Integrative Medicine, 2012,18(6):437-444.
Wang, Sx., Guo, H., Hu, Lm. et al. Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection., Chin. J. Integr. Med. 18, 437–444 (2012). https://doi.org/10.1007/s11655-012-1114-y DOI：

Shao-xia Wang, Hong Guo, Li-min Hu, et al. Caffeic acid ester fraction from Erigeron breviscapus inhibits microglial activation and provides neuroprotection[J]. Chinese Journal of Integrative Medicine, 2012,18(6):437-444. DOI： 10.1007/s11655-012-1114-y.

摘要

To investigate the effects of caffeic acid ester fraction (Caf) from Erigeron breviscapus

mainly composed of dicaffeoylquinic acids (diCQAs)

on microglial activation in vitro and focal cerebral ischemia in vivo. The production of nitric oxide (NO)

tumor necrosis factor α (TNF-α)

and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) treatment in rat primary cultured microglia were measured by Griess reaction or enzyme-linked immunosorbent assay. Cell viability of cortical neurons was measured using AlamarBlue reagent. The behavioral tests and the infarct area of brain were used to evaluate the damage to central nervous system in rat middle cerebral artery occlusion (MCAO) model of cerebral ischemia. Real time polymerase chain reaction was used to determine the expression of inducible nitric oxide synthase (iNOS)

TNF-α and IL-1β mRNA in ischemic cerebral tissues. Caf inhibited the production of NO

TNF-α and IL-1β induced by LPS treatment in primary microglia in a dose-dependent manner. Exposure of cortical neurons to conditioned medium from Caf-treated microglia increased neuronal cell viability (P<0.01) compared with conditioned medium from LPS-treated alone. In MCAO rat model of cerebral ischemia

Caf could significantly improve neurobehavioural performance and reduce percentage infarct volume compared with the vehicle group (P<0.05). Caf could also significantly inhibit the up-regulation of iNOS

TNF-α

and IL-1β gene expressions in ischemic cerebral tissues. Caf could suppress microglial activation

which may be one mechanism of its neuroprotective effect against ischemia.

Abstract

To investigate the effects of caffeic acid ester fraction (Caf) from Erigeron breviscapus

mainly composed of dicaffeoylquinic acids (diCQAs)

on microglial activation in vitro and focal cerebral ischemia in vivo. The production of nitric oxide (NO)

tumor necrosis factor α (TNF-α)

TNF-α and IL-1β mRNA in ischemic cerebral tissues. Caf inhibited the production of NO

TNF-α

and IL-1β gene expressions in ischemic cerebral tissues. Caf could suppress microglial activation

which may be one mechanism of its neuroprotective effect against ischemia.

关键词

microgliainflammationdicaffeoylquinic acidneuroprotcetion

Keywords

microgliainflammationdicaffeoylquinic acidneuroprotcetion

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